Haloterrigena daqingensis
Average proteome isoelectric point is 4.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3795 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N6X400|A0A1N6X400_9EURY Histidinol dehydrogenase OS=Haloterrigena daqingensis OX=588898 GN=hisD PE=3 SV=1
MM1 pKa = 7.2 VSYY4 pKa = 10.96 SPTLLEE10 pKa = 3.72 EE11 pKa = 4.62 HH12 pKa = 6.47 GEE14 pKa = 3.81 KK15 pKa = 10.26 RR16 pKa = 11.84 AIINFDD22 pKa = 3.86 RR23 pKa = 11.84 PDD25 pKa = 3.45 EE26 pKa = 4.22 YY27 pKa = 11.41 YY28 pKa = 10.85 GPCEE32 pKa = 3.92 TRR34 pKa = 11.84 SEE36 pKa = 4.32 KK37 pKa = 10.22 IAEE40 pKa = 4.2 LKK42 pKa = 9.73 EE43 pKa = 4.53 CCNSTHH49 pKa = 7.07 APLKK53 pKa = 10.8 DD54 pKa = 4.7 FISSTAGIEE63 pKa = 4.01 LLSDD67 pKa = 3.47 HH68 pKa = 6.78 WAGSTATVRR77 pKa = 11.84 IDD79 pKa = 3.74 FNTFDD84 pKa = 3.89 VDD86 pKa = 3.83 DD87 pKa = 5.68 LEE89 pKa = 5.66 DD90 pKa = 3.29 VDD92 pKa = 4.35 YY93 pKa = 11.18 VKK95 pKa = 10.91 SVVVDD100 pKa = 4.06 DD101 pKa = 4.22 GTLKK105 pKa = 10.69 INSDD109 pKa = 4.25 DD110 pKa = 4.24 GADD113 pKa = 3.36 TYY115 pKa = 11.77 LPGDD119 pKa = 3.75 VDD121 pKa = 3.33 WSLEE125 pKa = 3.79 QTQVHH130 pKa = 5.65 EE131 pKa = 4.49 FWTEE135 pKa = 3.48 YY136 pKa = 10.15 GARR139 pKa = 11.84 GEE141 pKa = 4.19 NVKK144 pKa = 10.61 VAVTDD149 pKa = 3.42 TGIDD153 pKa = 3.61 PDD155 pKa = 4.07 HH156 pKa = 6.96 EE157 pKa = 4.32 ALEE160 pKa = 5.29 LYY162 pKa = 10.22 TDD164 pKa = 4.41 DD165 pKa = 5.86 PDD167 pKa = 6.24 DD168 pKa = 3.55 EE169 pKa = 4.88 TYY171 pKa = 10.51 PGGWAEE177 pKa = 5.01 FDD179 pKa = 3.62 LDD181 pKa = 3.85 GNEE184 pKa = 4.8 IEE186 pKa = 6.14 DD187 pKa = 3.86 SEE189 pKa = 4.1 PWYY192 pKa = 10.24 DD193 pKa = 3.59 FWHH196 pKa = 6.79 GIEE199 pKa = 3.89 VSGPIKK205 pKa = 10.51 GKK207 pKa = 9.53 PPEE210 pKa = 4.48 SADD213 pKa = 3.51 EE214 pKa = 4.51 EE215 pKa = 5.11 YY216 pKa = 10.41 MGVAPEE222 pKa = 4.58 CDD224 pKa = 2.9 LMMVKK229 pKa = 9.94 MNDD232 pKa = 3.37 PDD234 pKa = 3.74 EE235 pKa = 4.71 TDD237 pKa = 3.5 TTTHH241 pKa = 5.91 SAMIAALEE249 pKa = 4.02 WCLEE253 pKa = 3.99 NGADD257 pKa = 4.94 IINSSWGNYY266 pKa = 7.84 ARR268 pKa = 11.84 QSPPYY273 pKa = 9.84 EE274 pKa = 4.66 LDD276 pKa = 2.96 LYY278 pKa = 11.67 YY279 pKa = 10.63 DD280 pKa = 4.13 IFQDD284 pKa = 4.25 ALDD287 pKa = 4.04 MNCISVGSSGNTDD300 pKa = 2.98 EE301 pKa = 5.14 GGEE304 pKa = 4.03 IGPAPGSYY312 pKa = 10.79 YY313 pKa = 10.74 NAISVGGVEE322 pKa = 5.1 EE323 pKa = 4.17 SGEE326 pKa = 4.35 MYY328 pKa = 10.66 GSSTGRR334 pKa = 11.84 TIEE337 pKa = 3.84 EE338 pKa = 4.48 SDD340 pKa = 2.76 WDD342 pKa = 4.26 EE343 pKa = 5.95 YY344 pKa = 10.23 PDD346 pKa = 3.8 EE347 pKa = 4.77 WPDD350 pKa = 3.33 EE351 pKa = 4.19 YY352 pKa = 10.53 IIPDD356 pKa = 3.36 ITAGGRR362 pKa = 11.84 NVPTTTEE369 pKa = 3.84 DD370 pKa = 3.6 DD371 pKa = 4.23 EE372 pKa = 5.01 YY373 pKa = 10.46 GTASGTSFAAPIVTGHH389 pKa = 6.62 IALLLSYY396 pKa = 10.82 SRR398 pKa = 11.84 TQTEE402 pKa = 3.59 SDD404 pKa = 3.32 ADD406 pKa = 3.59 INDD409 pKa = 4.2 IISALKK415 pKa = 8.03 GTAWKK420 pKa = 9.86 PDD422 pKa = 3.3 DD423 pKa = 3.93 WSDD426 pKa = 4.52 DD427 pKa = 3.71 DD428 pKa = 6.14 FEE430 pKa = 7.32 DD431 pKa = 4.78 GSEE434 pKa = 3.98 EE435 pKa = 4.24 AGEE438 pKa = 3.98 HH439 pKa = 5.26 DD440 pKa = 3.37 TRR442 pKa = 11.84 YY443 pKa = 10.24 GCGIAKK449 pKa = 10.13 VLDD452 pKa = 3.98 AQEE455 pKa = 4.53 KK456 pKa = 8.99 LRR458 pKa = 11.84 DD459 pKa = 3.58 MRR461 pKa = 11.84 SITGEE466 pKa = 4.08 VIFNSIPTNDD476 pKa = 3.26 AKK478 pKa = 11.36 VYY480 pKa = 10.85 VIDD483 pKa = 4.98 RR484 pKa = 11.84 NSNDD488 pKa = 3.19 LAGVLEE494 pKa = 4.28 PSEE497 pKa = 4.64 GEE499 pKa = 3.69 FRR501 pKa = 11.84 YY502 pKa = 10.43 EE503 pKa = 4.41 GGSSDD508 pKa = 3.87 DD509 pKa = 3.9 SYY511 pKa = 11.56 HH512 pKa = 7.25 IGVVADD518 pKa = 4.68 DD519 pKa = 4.29 DD520 pKa = 4.03
Molecular weight: 57.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.554
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.088
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.884
Patrickios 1.265
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|A0A1N7FFX8|A0A1N7FFX8_9EURY Uncharacterized protein OS=Haloterrigena daqingensis OX=588898 GN=BB347_17005 PE=4 SV=1
MM1 pKa = 7.43 GGVAGLSLVGFSGVASAGKK20 pKa = 9.98 NGRR23 pKa = 11.84 NGKK26 pKa = 9.08 NGKK29 pKa = 8.78 NGKK32 pKa = 9.45 NNGRR36 pKa = 11.84 NGKK39 pKa = 9.54 HH40 pKa = 5.94 NGRR43 pKa = 11.84 NGKK46 pKa = 9.08 NGNGRR51 pKa = 11.84 NGKK54 pKa = 9.89 RR55 pKa = 11.84 NGRR58 pKa = 11.84 NGKK61 pKa = 9.86 RR62 pKa = 11.84 NGRR65 pKa = 11.84 NGKK68 pKa = 9.19 NGNN71 pKa = 3.54
Molecular weight: 7.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.705
Grimsley 12.91
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.427
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3795
0
3795
1090797
29
1729
287.4
31.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.948 ± 0.055
0.764 ± 0.014
8.759 ± 0.074
9.505 ± 0.062
3.302 ± 0.03
8.092 ± 0.039
2.115 ± 0.02
4.595 ± 0.036
1.804 ± 0.028
8.851 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.78 ± 0.018
2.426 ± 0.024
4.491 ± 0.025
2.587 ± 0.025
6.039 ± 0.039
5.997 ± 0.034
6.561 ± 0.03
8.501 ± 0.041
1.129 ± 0.016
2.754 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here