Cladosporium cladosporioides virus 1
Average proteome isoelectric point is 7.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076JWY5|A0A076JWY5_9VIRU Uncharacterized protein OS=Cladosporium cladosporioides virus 1 OX=1529605 PE=4 SV=1
MM1 pKa = 7.84 ADD3 pKa = 3.94 LARR6 pKa = 11.84 LRR8 pKa = 11.84 HH9 pKa = 5.66 MLLDD13 pKa = 3.85 PTEE16 pKa = 4.3 SAVLLASMCHH26 pKa = 6.13 HH27 pKa = 7.77 LIPDD31 pKa = 3.85 PTSVDD36 pKa = 3.23 LFDD39 pKa = 4.51 YY40 pKa = 10.3 SFPPRR45 pKa = 11.84 ARR47 pKa = 11.84 DD48 pKa = 3.63 WIISAPFPLFGPAIPASKK66 pKa = 10.64 SSFEE70 pKa = 5.06 AILEE74 pKa = 4.01 PLRR77 pKa = 11.84 AMEE80 pKa = 4.16 YY81 pKa = 10.73 NSLDD85 pKa = 3.26 MAAVLMRR92 pKa = 11.84 SMRR95 pKa = 11.84 VADD98 pKa = 3.66 NPFAKK103 pKa = 9.08 VTRR106 pKa = 11.84 GEE108 pKa = 4.29 TTGDD112 pKa = 3.34 VAQSRR117 pKa = 11.84 AGAAQHH123 pKa = 6.7 AAQSPIEE130 pKa = 3.9 FAIYY134 pKa = 10.34 DD135 pKa = 3.74 VLRR138 pKa = 11.84 DD139 pKa = 3.45 AGYY142 pKa = 9.12 TSVSDD147 pKa = 4.43 ALSKK151 pKa = 10.73 LQSFLTSTKK160 pKa = 10.77 AFVHH164 pKa = 6.73 PITRR168 pKa = 11.84 QFSRR172 pKa = 11.84 HH173 pKa = 4.65 RR174 pKa = 11.84 SAALFLINKK183 pKa = 8.37 PGARR187 pKa = 11.84 GPTAFLTPFFTSKK200 pKa = 10.59 EE201 pKa = 4.07 ATKK204 pKa = 10.85 LFAAVVFRR212 pKa = 11.84 SDD214 pKa = 2.82 ILTSLSRR221 pKa = 11.84 LNAARR226 pKa = 11.84 GVTVLGYY233 pKa = 10.24 AARR236 pKa = 11.84 ASLEE240 pKa = 3.74 AVRR243 pKa = 11.84 RR244 pKa = 11.84 IVEE247 pKa = 3.92 PAHH250 pKa = 6.77 AIVNEE255 pKa = 4.01 YY256 pKa = 11.05 SFDD259 pKa = 3.8 PQTLSFIDD267 pKa = 3.86 PAGDD271 pKa = 3.45 PTYY274 pKa = 10.36 HH275 pKa = 5.85 ITDD278 pKa = 3.86 RR279 pKa = 11.84 TPSVVVAFGRR289 pKa = 11.84 VLSGGSVDD297 pKa = 3.85 ACSKK301 pKa = 10.97 ASDD304 pKa = 4.02 LRR306 pKa = 11.84 TSLDD310 pKa = 3.33 IPGGLPDD317 pKa = 3.28 VARR320 pKa = 11.84 EE321 pKa = 4.08 YY322 pKa = 10.93 FSSTGNVEE330 pKa = 3.82 DD331 pKa = 4.88 TIRR334 pKa = 11.84 SAATALSDD342 pKa = 3.46 FRR344 pKa = 11.84 SFRR347 pKa = 11.84 DD348 pKa = 3.41 SHH350 pKa = 6.58 PGGHH354 pKa = 6.32 EE355 pKa = 4.03 LNKK358 pKa = 10.82 DD359 pKa = 3.15 SGLSFFSRR367 pKa = 11.84 DD368 pKa = 2.79 RR369 pKa = 11.84 ATRR372 pKa = 11.84 DD373 pKa = 3.06 RR374 pKa = 11.84 NTAACITRR382 pKa = 11.84 VEE384 pKa = 4.36 EE385 pKa = 4.35 VACLLRR391 pKa = 11.84 SQGRR395 pKa = 11.84 DD396 pKa = 2.88 IARR399 pKa = 11.84 MAFVVEE405 pKa = 4.03 WGGPLNNDD413 pKa = 3.99 SILAALALTGATACVDD429 pKa = 3.55 VGPGAFKK436 pKa = 11.03 VGDD439 pKa = 3.94 SSEE442 pKa = 4.1 EE443 pKa = 3.78 LATEE447 pKa = 4.06 NDD449 pKa = 3.01 KK450 pKa = 11.33 GAYY453 pKa = 8.7 VCLIPRR459 pKa = 11.84 ASTRR463 pKa = 11.84 GLPRR467 pKa = 11.84 MPIVGYY473 pKa = 10.41 EE474 pKa = 4.13 SGDD477 pKa = 3.8 SVTQRR482 pKa = 11.84 LDD484 pKa = 3.12 RR485 pKa = 11.84 LLSFISGPDD494 pKa = 3.09 NYY496 pKa = 10.51 PPVAYY501 pKa = 8.43 ITGGSTTSGAAPADD515 pKa = 3.61 IIGITQARR523 pKa = 11.84 AALLTDD529 pKa = 3.66 YY530 pKa = 10.42 LASGSFFCGDD540 pKa = 3.0 ILIPDD545 pKa = 4.6 VCRR548 pKa = 11.84 HH549 pKa = 6.14 PPDD552 pKa = 3.94 GTDD555 pKa = 4.85 DD556 pKa = 3.75 EE557 pKa = 5.39 CLACANFDD565 pKa = 3.62 YY566 pKa = 11.29 SVFTVGDD573 pKa = 3.59 VCQLDD578 pKa = 4.02 GFSLVKK584 pKa = 10.14 PRR586 pKa = 11.84 ASYY589 pKa = 10.79 AHH591 pKa = 6.52 NLHH594 pKa = 7.1 LSLEE598 pKa = 4.62 WIHH601 pKa = 6.01 GAARR605 pKa = 11.84 EE606 pKa = 4.03 FSEE609 pKa = 5.04 SRR611 pKa = 11.84 TLLALDD617 pKa = 3.78 SVVATNVARR626 pKa = 11.84 NADD629 pKa = 3.2 WGDD632 pKa = 3.41 FVPRR636 pKa = 11.84 DD637 pKa = 3.72 GPRR640 pKa = 11.84 RR641 pKa = 11.84 PHH643 pKa = 6.24 HH644 pKa = 6.56 PSYY647 pKa = 10.81 GLKK650 pKa = 10.12 VKK652 pKa = 10.64 LIIEE656 pKa = 4.09 DD657 pKa = 3.92 VYY659 pKa = 11.49 RR660 pKa = 11.84 SMAEE664 pKa = 3.77 GAEE667 pKa = 4.1 EE668 pKa = 5.18 GIGATKK674 pKa = 10.1 TIVEE678 pKa = 4.44 SLAA681 pKa = 3.57
Molecular weight: 73.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.454
IPC2_protein 5.385
IPC_protein 5.41
Toseland 5.677
ProMoST 5.69
Dawson 5.588
Bjellqvist 5.639
Wikipedia 5.563
Rodwell 5.537
Grimsley 5.766
Solomon 5.588
Lehninger 5.563
Nozaki 5.804
DTASelect 6.008
Thurlkill 5.893
EMBOSS 5.868
Sillero 5.893
Patrickios 4.151
IPC_peptide 5.601
IPC2_peptide 5.906
IPC2.peptide.svr19 5.847
Protein with the highest isoelectric point:
>tr|A0A076JYE6|A0A076JYE6_9VIRU Putative RNA-dependent RNA polymerase OS=Cladosporium cladosporioides virus 1 OX=1529605 PE=4 SV=1
MM1 pKa = 7.68 SFEE4 pKa = 4.03 RR5 pKa = 11.84 SAVNSWARR13 pKa = 11.84 SVPVFANPDD22 pKa = 3.74 SPTSPAAGRR31 pKa = 11.84 RR32 pKa = 11.84 EE33 pKa = 3.88 EE34 pKa = 4.46 SPAARR39 pKa = 11.84 HH40 pKa = 5.79 GATPPRR46 pKa = 11.84 GRR48 pKa = 11.84 PAAVNPRR55 pKa = 11.84 SSGQPPPVINKK66 pKa = 9.11 HH67 pKa = 4.46 VTHH70 pKa = 6.41 QGSSRR75 pKa = 11.84 VGGVTVYY82 pKa = 10.22 PGSSVSQDD90 pKa = 3.07 PEE92 pKa = 3.79 RR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 QRR97 pKa = 11.84 YY98 pKa = 7.47 HH99 pKa = 6.74 HH100 pKa = 6.82 SPPEE104 pKa = 3.75 HH105 pKa = 6.41 GRR107 pKa = 11.84 RR108 pKa = 11.84 DD109 pKa = 3.78 SSPHH113 pKa = 5.61 GSGPPGSRR121 pKa = 11.84 AGSSATHH128 pKa = 6.66 EE129 pKa = 4.21 ISRR132 pKa = 11.84 GSSLHH137 pKa = 5.79 RR138 pKa = 11.84 RR139 pKa = 11.84 ADD141 pKa = 3.62 FGQRR145 pKa = 11.84 AVEE148 pKa = 3.95 AGRR151 pKa = 11.84 PVGYY155 pKa = 9.69 SSRR158 pKa = 11.84 RR159 pKa = 11.84 RR160 pKa = 11.84 DD161 pKa = 3.55 TVFSDD166 pKa = 4.01 AARR169 pKa = 11.84 MVTVPEE175 pKa = 4.6 GGFVEE180 pKa = 5.09 YY181 pKa = 10.48 EE182 pKa = 4.24 SQTVQHH188 pKa = 6.73 TDD190 pKa = 2.97 GTSVTTEE197 pKa = 3.74 RR198 pKa = 11.84 VSVSSQGSRR207 pKa = 11.84 RR208 pKa = 11.84 SHH210 pKa = 6.47 RR211 pKa = 11.84 SNPRR215 pKa = 11.84 KK216 pKa = 10.1 VKK218 pKa = 9.97 EE219 pKa = 4.04 KK220 pKa = 10.45 RR221 pKa = 11.84 DD222 pKa = 3.65 GNEE225 pKa = 3.45 KK226 pKa = 9.82 RR227 pKa = 11.84 KK228 pKa = 10.48 KK229 pKa = 10.5 RR230 pKa = 11.84 MTIGEE235 pKa = 4.18 WMQTT239 pKa = 3.3
Molecular weight: 26.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.188
IPC2_protein 9.926
IPC_protein 11.286
Toseland 11.403
ProMoST 11.857
Dawson 11.418
Bjellqvist 11.389
Wikipedia 11.871
Rodwell 11.199
Grimsley 11.462
Solomon 11.871
Lehninger 11.784
Nozaki 11.403
DTASelect 11.389
Thurlkill 11.403
EMBOSS 11.901
Sillero 11.418
Patrickios 10.921
IPC_peptide 11.886
IPC2_peptide 10.847
IPC2.peptide.svr19 9.467
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2593
239
760
518.6
55.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.567 ± 0.892
0.887 ± 0.235
6.363 ± 0.392
4.512 ± 0.336
3.625 ± 0.442
8.099 ± 0.379
2.468 ± 0.299
3.702 ± 0.377
3.355 ± 0.77
8.793 ± 1.101
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.005 ± 0.315
2.43 ± 0.283
7.096 ± 0.509
2.198 ± 0.242
8.176 ± 0.702
9.603 ± 0.57
5.631 ± 0.287
7.443 ± 0.579
0.733 ± 0.132
2.314 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here