Gordonia phage Yago84
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y6EHX6|A0A4Y6EHX6_9CAUD Uncharacterized protein OS=Gordonia phage Yago84 OX=2571254 GN=57 PE=4 SV=1
MM1 pKa = 7.2 SQTTVTTEE9 pKa = 3.64 QLEE12 pKa = 4.43 QIAEE16 pKa = 4.56 LIDD19 pKa = 4.02 AEE21 pKa = 4.44 LGEE24 pKa = 5.6 DD25 pKa = 3.82 IRR27 pKa = 11.84 PDD29 pKa = 3.2 YY30 pKa = 10.79 SGRR33 pKa = 11.84 FMYY36 pKa = 10.33 GRR38 pKa = 11.84 SCVGYY43 pKa = 10.48 VGAEE47 pKa = 3.87 CSQFALLLAAATCGYY62 pKa = 10.24 EE63 pKa = 4.18 LDD65 pKa = 3.77 AKK67 pKa = 11.07 VSIEE71 pKa = 3.87 ALLWAIGDD79 pKa = 3.94 LGEE82 pKa = 4.93 PSTDD86 pKa = 2.82 SMGRR90 pKa = 11.84 DD91 pKa = 3.44 TIFYY95 pKa = 8.74 WRR97 pKa = 11.84 DD98 pKa = 3.08 VRR100 pKa = 11.84 VDD102 pKa = 3.63 DD103 pKa = 6.15 AEE105 pKa = 4.22 DD106 pKa = 3.06
Molecular weight: 11.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.859
IPC_protein 3.808
Toseland 3.605
ProMoST 3.91
Dawson 3.795
Bjellqvist 3.999
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.923
Patrickios 1.875
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|A0A4Y6EQ25|A0A4Y6EQ25_9CAUD Uncharacterized protein OS=Gordonia phage Yago84 OX=2571254 GN=58 PE=4 SV=1
MM1 pKa = 6.92 TTTEE5 pKa = 4.2 PVSKK9 pKa = 10.66 YY10 pKa = 10.1 GAYY13 pKa = 10.05 DD14 pKa = 3.17 VEE16 pKa = 4.38 RR17 pKa = 11.84 AQRR20 pKa = 11.84 VAEE23 pKa = 4.06 LTRR26 pKa = 11.84 AGLSARR32 pKa = 11.84 EE33 pKa = 3.41 IAARR37 pKa = 11.84 LRR39 pKa = 11.84 VTVRR43 pKa = 11.84 TVGRR47 pKa = 11.84 DD48 pKa = 3.11 RR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 TGTNGHH57 pKa = 6.45 RR58 pKa = 11.84 PARR61 pKa = 11.84 WSEE64 pKa = 3.95 EE65 pKa = 3.43 KK66 pKa = 10.52 LARR69 pKa = 11.84 AKK71 pKa = 10.85 ALLDD75 pKa = 4.19 DD76 pKa = 4.37 GASYY80 pKa = 11.13 RR81 pKa = 11.84 EE82 pKa = 3.73 TAMTVGCDD90 pKa = 3.29 PTHH93 pKa = 6.55 LRR95 pKa = 11.84 RR96 pKa = 11.84 RR97 pKa = 11.84 FPGYY101 pKa = 9.94 GWPPGHH107 pKa = 6.71 WPEE110 pKa = 4.34 GLNPQIIAAARR121 pKa = 11.84 RR122 pKa = 11.84 EE123 pKa = 4.16 KK124 pKa = 10.98
Molecular weight: 13.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.736
IPC_protein 10.891
Toseland 10.862
ProMoST 10.994
Dawson 10.935
Bjellqvist 10.789
Wikipedia 11.272
Rodwell 10.862
Grimsley 10.994
Solomon 11.213
Lehninger 11.14
Nozaki 10.847
DTASelect 10.789
Thurlkill 10.862
EMBOSS 11.301
Sillero 10.891
Patrickios 10.599
IPC_peptide 11.213
IPC2_peptide 10.058
IPC2.peptide.svr19 8.748
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
83
0
83
19346
45
1828
233.1
25.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.437 ± 0.252
0.92 ± 0.146
6.963 ± 0.281
5.96 ± 0.273
2.383 ± 0.157
8.73 ± 0.419
1.897 ± 0.193
4.352 ± 0.249
2.254 ± 0.163
8.203 ± 0.229
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.445 ± 0.109
2.698 ± 0.191
5.707 ± 0.267
3.453 ± 0.22
7.056 ± 0.373
5.619 ± 0.208
6.926 ± 0.213
7.836 ± 0.192
2.021 ± 0.096
2.14 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here