Candidatus Photodesmus katoptron Akat1
Average proteome isoelectric point is 7.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 853 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S3DKF3|S3DKF3_9GAMM Metallo-beta-lactamase OS=Candidatus Photodesmus katoptron Akat1 OX=1236703 GN=O1U_0068 PE=4 SV=1
MM1 pKa = 7.26 MEE3 pKa = 4.54 SLYY6 pKa = 10.49 ILIPIGIILIYY17 pKa = 10.27 LAMIIFFWAVKK28 pKa = 9.22 TGQFEE33 pKa = 5.03 DD34 pKa = 4.78 LDD36 pKa = 4.03 QEE38 pKa = 4.53 GCKK41 pKa = 9.76 ILFLDD46 pKa = 4.47 EE47 pKa = 4.47 NNNN50 pKa = 3.49
Molecular weight: 5.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.821
IPC2_protein 4.037
IPC_protein 3.783
Toseland 3.63
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.567
Solomon 3.732
Lehninger 3.681
Nozaki 3.935
DTASelect 4.037
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.732
IPC2_peptide 3.884
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|S3DKG0|S3DKG0_9GAMM L-serine dehydratase OS=Candidatus Photodesmus katoptron Akat1 OX=1236703 GN=O1U_0073 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.5 RR3 pKa = 11.84 TFQPTVLKK11 pKa = 10.55 RR12 pKa = 11.84 SRR14 pKa = 11.84 THH16 pKa = 6.38 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 KK29 pKa = 9.18 IINARR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.12 KK37 pKa = 10.75 GRR39 pKa = 11.84 VHH41 pKa = 7.34 LSKK44 pKa = 11.11
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.676
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.398
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
853
0
853
279869
37
1618
328.1
37.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.771 ± 0.065
1.129 ± 0.03
4.54 ± 0.053
5.666 ± 0.061
4.597 ± 0.06
5.944 ± 0.07
2.124 ± 0.036
9.997 ± 0.072
7.924 ± 0.092
10.401 ± 0.083
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.389 ± 0.035
5.922 ± 0.071
3.293 ± 0.038
3.684 ± 0.05
4.411 ± 0.061
7.332 ± 0.066
4.87 ± 0.05
5.693 ± 0.073
0.981 ± 0.03
3.333 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here