Martelella sp. AD-3
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4480 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A127CND3|A0A127CND3_9RHIZ C4-dicarboxylate ABC transporter substrate-binding protein OS=Martelella sp. AD-3 OX=686597 GN=AZF01_20110 PE=3 SV=1
MM1 pKa = 6.69 TTYY4 pKa = 11.08 SLDD7 pKa = 3.9 DD8 pKa = 4.22 DD9 pKa = 5.49 PIVAVIDD16 pKa = 4.47 AYY18 pKa = 10.74 DD19 pKa = 3.4 VSDD22 pKa = 4.38 NDD24 pKa = 3.25 VALYY28 pKa = 10.53 AFRR31 pKa = 11.84 SSEE34 pKa = 3.8 GLTEE38 pKa = 4.02 NMPTTGRR45 pKa = 11.84 AIYY48 pKa = 9.93 HH49 pKa = 6.27 GEE51 pKa = 3.67 HH52 pKa = 6.4 SGFAFQSDD60 pKa = 4.03 GSSGAISDD68 pKa = 3.98 TFGATVNFGEE78 pKa = 4.48 GTLYY82 pKa = 11.08 GSIYY86 pKa = 9.8 PDD88 pKa = 3.81 GPNQRR93 pKa = 11.84 ISFDD97 pKa = 3.3 GTINGAEE104 pKa = 4.3 FTSDD108 pKa = 3.23 PGTIALFEE116 pKa = 4.31 SEE118 pKa = 4.4 YY119 pKa = 10.66 TPSGVNRR126 pKa = 11.84 IYY128 pKa = 11.22 DD129 pKa = 4.03 DD130 pKa = 3.83 DD131 pKa = 4.12 TSVINQNTSAVEE143 pKa = 3.84 GAFYY147 pKa = 11.25 GDD149 pKa = 3.23 AAKK152 pKa = 11.02 AMGGTYY158 pKa = 10.49 VIDD161 pKa = 3.9 GQGAKK166 pKa = 10.04 EE167 pKa = 3.97 GAHH170 pKa = 6.71 LIGGFKK176 pKa = 10.38 ADD178 pKa = 3.51 RR179 pKa = 4.23
Molecular weight: 18.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.884
IPC_protein 3.872
Toseland 3.656
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.694
Grimsley 3.567
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.24
Thurlkill 3.719
EMBOSS 3.821
Sillero 3.999
Patrickios 1.074
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.874
Protein with the highest isoelectric point:
>tr|A0A127CJR8|A0A127CJR8_9RHIZ 4-hydroxyphenylacetate 3-monooxygenase OS=Martelella sp. AD-3 OX=686597 GN=AZF01_11705 PE=4 SV=1
MM1 pKa = 6.51 STKK4 pKa = 9.4 RR5 pKa = 11.84 TYY7 pKa = 10.43 QPSKK11 pKa = 9.73 LVRR14 pKa = 11.84 KK15 pKa = 9.15 RR16 pKa = 11.84 RR17 pKa = 11.84 HH18 pKa = 4.42 GFRR21 pKa = 11.84 ARR23 pKa = 11.84 MATKK27 pKa = 10.22 GGRR30 pKa = 11.84 KK31 pKa = 8.91 VLQARR36 pKa = 11.84 RR37 pKa = 11.84 ARR39 pKa = 11.84 GRR41 pKa = 11.84 KK42 pKa = 9.03 RR43 pKa = 11.84 LSAA46 pKa = 4.03
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4480
0
4480
1449651
40
5037
323.6
35.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.175 ± 0.046
0.81 ± 0.011
5.916 ± 0.031
6.132 ± 0.041
4.058 ± 0.024
8.63 ± 0.051
1.943 ± 0.017
5.537 ± 0.03
3.346 ± 0.027
9.886 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.702 ± 0.018
2.814 ± 0.023
4.923 ± 0.025
2.788 ± 0.019
6.693 ± 0.045
5.478 ± 0.03
5.251 ± 0.029
7.215 ± 0.029
1.317 ± 0.016
2.387 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here