Canis familiaris papillomavirus 17
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U4JWN8|A0A0U4JWN8_9PAPI Regulatory protein E2 OS=Canis familiaris papillomavirus 17 OX=1778550 GN=E2 PE=3 SV=1
MM1 pKa = 7.09 QPTMRR6 pKa = 11.84 AKK8 pKa = 9.8 RR9 pKa = 11.84 VKK11 pKa = 10.08 RR12 pKa = 11.84 ASAEE16 pKa = 3.9 QLYY19 pKa = 7.93 KK20 pKa = 10.26 TCQQGGDD27 pKa = 4.38 CIPDD31 pKa = 3.22 VVAKK35 pKa = 10.64 YY36 pKa = 7.32 EE37 pKa = 4.27 HH38 pKa = 6.89 KK39 pKa = 9.8 TPADD43 pKa = 4.14 KK44 pKa = 10.45 ILQIGGSVIYY54 pKa = 10.68 LGGLSIGTGKK64 pKa = 8.72 GTGGSTGYY72 pKa = 10.33 RR73 pKa = 11.84 PLGVDD78 pKa = 3.33 VNASTTRR85 pKa = 11.84 PVLPRR90 pKa = 11.84 PAIPTEE96 pKa = 4.18 TIGVDD101 pKa = 3.32 VGSGTVSAGEE111 pKa = 3.94 SSIIPLLEE119 pKa = 4.69 AGAGGDD125 pKa = 3.47 AVTISPEE132 pKa = 4.0 VPTITEE138 pKa = 4.17 DD139 pKa = 3.98 LVINPSSGAPTVTTAAGEE157 pKa = 4.53 GEE159 pKa = 4.49 SVAVLEE165 pKa = 4.7 VGTTPSTNSRR175 pKa = 11.84 VTSTSQHH182 pKa = 5.59 TNPSFTPVFHH192 pKa = 6.72 STPQPGEE199 pKa = 3.72 ASTLEE204 pKa = 4.4 SIVVGHH210 pKa = 5.21 STGGRR215 pKa = 11.84 VVDD218 pKa = 4.39 VYY220 pKa = 11.28 EE221 pKa = 5.16 EE222 pKa = 4.27 IPLDD226 pKa = 3.77 DD227 pKa = 5.03 FGPSEE232 pKa = 5.09 FEE234 pKa = 4.56 IEE236 pKa = 4.35 DD237 pKa = 3.64 PTPRR241 pKa = 11.84 TSTPRR246 pKa = 11.84 SAVTNLVQRR255 pKa = 11.84 ARR257 pKa = 11.84 QFYY260 pKa = 9.4 NRR262 pKa = 11.84 RR263 pKa = 11.84 FAQVQVTNEE272 pKa = 3.86 AFLSRR277 pKa = 11.84 PASLVEE283 pKa = 4.2 FEE285 pKa = 4.56 NPAYY289 pKa = 10.13 QPEE292 pKa = 4.35 EE293 pKa = 4.16 TVVFPAGSGEE303 pKa = 4.19 PLAAPDD309 pKa = 4.33 PDD311 pKa = 3.91 FADD314 pKa = 3.24 VRR316 pKa = 11.84 VLNRR320 pKa = 11.84 AVFQANPRR328 pKa = 11.84 GGVRR332 pKa = 11.84 VSRR335 pKa = 11.84 LGTKK339 pKa = 8.05 NTIRR343 pKa = 11.84 LRR345 pKa = 11.84 SGTHH349 pKa = 5.69 IGGSTHH355 pKa = 5.97 YY356 pKa = 10.13 FYY358 pKa = 11.21 DD359 pKa = 3.81 LSSISLQPEE368 pKa = 3.66 IEE370 pKa = 4.09 LSVLGEE376 pKa = 3.99 HH377 pKa = 6.41 SGEE380 pKa = 4.18 SEE382 pKa = 3.93 IVLGSTTSTVIDD394 pKa = 3.73 AVNVEE399 pKa = 4.34 GTISVPEE406 pKa = 4.01 EE407 pKa = 3.79 DD408 pKa = 4.02 VLLDD412 pKa = 3.85 EE413 pKa = 4.39 YY414 pKa = 11.87 VEE416 pKa = 4.8 DD417 pKa = 4.97 FSHH420 pKa = 6.72 SQLALSGGSRR430 pKa = 11.84 SQSRR434 pKa = 11.84 VVDD437 pKa = 3.25 IPEE440 pKa = 4.06 FQRR443 pKa = 11.84 EE444 pKa = 4.23 VPKK447 pKa = 10.93 GSVIIDD453 pKa = 3.89 DD454 pKa = 4.77 LSDD457 pKa = 4.36 SIHH460 pKa = 5.73 VAHH463 pKa = 7.22 PGGRR467 pKa = 11.84 DD468 pKa = 3.05 TTAEE472 pKa = 4.21 PFSPPHH478 pKa = 6.8 SSDD481 pKa = 3.68 VPSSSSDD488 pKa = 3.54 SVSSTFDD495 pKa = 3.1 LHH497 pKa = 7.06 PSLLKK502 pKa = 9.84 RR503 pKa = 11.84 RR504 pKa = 11.84 KK505 pKa = 9.48 RR506 pKa = 11.84 KK507 pKa = 10.22 RR508 pKa = 11.84 GDD510 pKa = 2.76 II511 pKa = 3.87
Molecular weight: 54.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.948
IPC2_protein 4.914
IPC_protein 4.876
Toseland 4.787
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.978
Wikipedia 4.711
Rodwell 4.762
Grimsley 4.711
Solomon 4.851
Lehninger 4.8
Nozaki 4.965
DTASelect 5.105
Thurlkill 4.787
EMBOSS 4.749
Sillero 5.041
Patrickios 4.164
IPC_peptide 4.851
IPC2_peptide 5.029
IPC2.peptide.svr19 4.984
Protein with the highest isoelectric point:
>tr|A0A0U4K543|A0A0U4K543_9PAPI Protein E6 OS=Canis familiaris papillomavirus 17 OX=1778550 GN=E6 PE=3 SV=1
MM1 pKa = 7.22 MEE3 pKa = 4.25 ALVRR7 pKa = 11.84 RR8 pKa = 11.84 CDD10 pKa = 3.56 VLQEE14 pKa = 4.46 VILQHH19 pKa = 5.74 YY20 pKa = 8.31 EE21 pKa = 3.91 RR22 pKa = 11.84 GSHH25 pKa = 6.99 KK26 pKa = 10.84 LSDD29 pKa = 3.29 QCNYY33 pKa = 9.49 WEE35 pKa = 4.02 ATRR38 pKa = 11.84 RR39 pKa = 11.84 EE40 pKa = 4.14 STLLHH45 pKa = 6.43 FARR48 pKa = 11.84 KK49 pKa = 9.21 NRR51 pKa = 11.84 IHH53 pKa = 7.13 RR54 pKa = 11.84 LGVIPVPALATTEE67 pKa = 4.32 ANAKK71 pKa = 9.9 KK72 pKa = 9.93 AIQMCLVLSSLNQSPYY88 pKa = 10.12 RR89 pKa = 11.84 DD90 pKa = 4.35 EE91 pKa = 4.49 PWTMTDD97 pKa = 2.9 TSRR100 pKa = 11.84 EE101 pKa = 3.91 MWEE104 pKa = 4.45 APPKK108 pKa = 10.49 GCLKK112 pKa = 10.67 KK113 pKa = 10.95 GGVTVDD119 pKa = 3.23 VLFDD123 pKa = 4.02 GDD125 pKa = 4.15 PNNVFPYY132 pKa = 9.29 TLWSYY137 pKa = 11.14 IYY139 pKa = 9.79 YY140 pKa = 10.55 QNLNDD145 pKa = 3.63 EE146 pKa = 4.72 WIKK149 pKa = 11.04 VPGKK153 pKa = 10.19 VDD155 pKa = 3.53 YY156 pKa = 10.97 EE157 pKa = 4.09 GLYY160 pKa = 8.79 YY161 pKa = 10.33 TDD163 pKa = 4.02 EE164 pKa = 5.82 DD165 pKa = 4.29 GEE167 pKa = 4.38 KK168 pKa = 9.59 IYY170 pKa = 11.08 YY171 pKa = 8.94 EE172 pKa = 4.18 QFAKK176 pKa = 10.87 DD177 pKa = 3.27 AMRR180 pKa = 11.84 FGNTGTWKK188 pKa = 9.91 VRR190 pKa = 11.84 YY191 pKa = 9.45 KK192 pKa = 10.26 NTVLSALVSSSGSSWPTSAPTSNSATASTTTAQRR226 pKa = 11.84 EE227 pKa = 4.71 GPTDD231 pKa = 3.69 TPASSATSSSVSSACSSTSTGNRR254 pKa = 11.84 GGPGQLIVSEE264 pKa = 4.38 SQQGGSEE271 pKa = 4.17 GGRR274 pKa = 11.84 GRR276 pKa = 11.84 GRR278 pKa = 11.84 GRR280 pKa = 11.84 GRR282 pKa = 11.84 GRR284 pKa = 11.84 GKK286 pKa = 10.56 RR287 pKa = 11.84 GGSPSPPLFVHH298 pKa = 6.59 SDD300 pKa = 3.35 WGCRR304 pKa = 11.84 EE305 pKa = 3.89 PQGSEE310 pKa = 3.22 KK311 pKa = 10.29 RR312 pKa = 11.84 ARR314 pKa = 11.84 EE315 pKa = 3.92 ASSDD319 pKa = 3.78 GEE321 pKa = 5.34 DD322 pKa = 3.15 RR323 pKa = 11.84 ATWGGGRR330 pKa = 11.84 EE331 pKa = 3.7 RR332 pKa = 11.84 RR333 pKa = 11.84 AGQGEE338 pKa = 4.17 RR339 pKa = 11.84 PSCSSCNLSPNPSTIPTLSTPGPTPGHH366 pKa = 5.33 GHH368 pKa = 7.03 GGRR371 pKa = 11.84 PTPKK375 pKa = 10.26 APRR378 pKa = 11.84 LSPAVPLEE386 pKa = 3.88 QLVQRR391 pKa = 11.84 PGDD394 pKa = 3.5 FEE396 pKa = 4.73 TEE398 pKa = 3.72 VGRR401 pKa = 11.84 RR402 pKa = 11.84 PRR404 pKa = 11.84 GPEE407 pKa = 3.81 GQNRR411 pKa = 11.84 PYY413 pKa = 10.47 PVIIVKK419 pKa = 10.7 GDD421 pKa = 3.42 GNASKK426 pKa = 10.44 CWRR429 pKa = 11.84 RR430 pKa = 11.84 RR431 pKa = 11.84 LRR433 pKa = 11.84 LRR435 pKa = 11.84 YY436 pKa = 9.82 GEE438 pKa = 5.19 LYY440 pKa = 9.78 TAISTGFSWTTANGPHH456 pKa = 6.22 RR457 pKa = 11.84 VCRR460 pKa = 11.84 PRR462 pKa = 11.84 MVIAFDD468 pKa = 3.93 SDD470 pKa = 3.4 EE471 pKa = 3.85 QRR473 pKa = 11.84 AAFLKK478 pKa = 10.31 NVRR481 pKa = 11.84 IPKK484 pKa = 9.95 VLDD487 pKa = 3.1 IAFGSLDD494 pKa = 3.52 SLL496 pKa = 4.34
Molecular weight: 54.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.81
IPC2_protein 8.668
IPC_protein 8.624
Toseland 9.063
ProMoST 9.063
Dawson 9.458
Bjellqvist 9.37
Wikipedia 9.633
Rodwell 9.56
Grimsley 9.487
Solomon 9.502
Lehninger 9.458
Nozaki 9.472
DTASelect 9.253
Thurlkill 9.326
EMBOSS 9.575
Sillero 9.516
Patrickios 4.774
IPC_peptide 9.502
IPC2_peptide 8.317
IPC2.peptide.svr19 7.758
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2006
98
511
334.3
36.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.334 ± 0.479
2.293 ± 0.79
5.733 ± 0.487
6.431 ± 0.568
3.24 ± 0.453
8.475 ± 0.737
2.044 ± 0.359
4.038 ± 0.409
4.487 ± 0.922
7.527 ± 0.574
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.496 ± 0.407
3.639 ± 0.741
8.624 ± 1.438
3.789 ± 0.434
7.228 ± 0.834
8.225 ± 1.338
6.78 ± 0.787
6.73 ± 1.234
1.196 ± 0.394
2.692 ± 0.626
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here