Actinoplanes derwentensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales<

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9207 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2CKE1|A0A1H2CKE1_9ACTN Uncharacterized conserved protein DUF58 family contains vWF domain OS=Actinoplanes derwentensis OX=113562 GN=SAMN04489716_5974 PE=4 SV=1
MM1 pKa = 7.47GVQDD5 pKa = 4.45EE6 pKa = 5.42PIDD9 pKa = 4.19PFNGDD14 pKa = 3.56PTDD17 pKa = 3.89PAAGLDD23 pKa = 4.1DD24 pKa = 4.92LNEE27 pKa = 4.47DD28 pKa = 4.32AEE30 pKa = 4.54SEE32 pKa = 4.14PLTEE36 pKa = 6.16DD37 pKa = 2.91EE38 pKa = 4.55RR39 pKa = 11.84QDD41 pKa = 3.73VLEE44 pKa = 5.08DD45 pKa = 3.7LSDD48 pKa = 4.04LEE50 pKa = 4.86IYY52 pKa = 9.98QALLSPTGIRR62 pKa = 11.84GLVIEE67 pKa = 5.58CEE69 pKa = 4.47DD70 pKa = 3.68CHH72 pKa = 6.56EE73 pKa = 4.0PHH75 pKa = 6.71YY76 pKa = 10.76FDD78 pKa = 4.51WDD80 pKa = 3.86LLRR83 pKa = 11.84GNLRR87 pKa = 11.84HH88 pKa = 6.15LLSSGRR94 pKa = 11.84PRR96 pKa = 11.84VHH98 pKa = 6.65EE99 pKa = 4.06PAYY102 pKa = 10.8DD103 pKa = 4.24PDD105 pKa = 4.78PDD107 pKa = 5.1HH108 pKa = 6.67YY109 pKa = 10.69VTWEE113 pKa = 3.91YY114 pKa = 11.54ARR116 pKa = 11.84GYY118 pKa = 10.77ADD120 pKa = 3.94GVHH123 pKa = 6.44DD124 pKa = 4.42TLTEE128 pKa = 4.28GSDD131 pKa = 5.29DD132 pKa = 3.79EE133 pKa = 4.61AQQ135 pKa = 3.24

Molecular weight: 15.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2D435|A0A1H2D435_9ACTN Uncharacterized protein OS=Actinoplanes derwentensis OX=113562 GN=SAMN04489716_7910 PE=4 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9207

0

9207

3147326

29

14024

341.8

36.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.42 ± 0.044

0.692 ± 0.008

6.094 ± 0.023

5.039 ± 0.024

2.843 ± 0.014

9.191 ± 0.029

2.144 ± 0.013

3.804 ± 0.019

1.935 ± 0.021

10.319 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.722 ± 0.01

1.997 ± 0.017

6.052 ± 0.028

2.834 ± 0.016

7.815 ± 0.033

5.291 ± 0.021

6.596 ± 0.031

8.575 ± 0.027

1.597 ± 0.011

2.041 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski