Ornithinimicrobium cerasi
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3368 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A285VPA4|A0A285VPA4_9MICO Proteasome accessory factor C OS=Ornithinimicrobium cerasi OX=2248773 GN=SAMN05421879_103127 PE=4 SV=1
MM1 pKa = 7.96 RR2 pKa = 11.84 NRR4 pKa = 11.84 SVGALASAAALALLLTSCAEE24 pKa = 4.15 SDD26 pKa = 4.41 RR27 pKa = 11.84 DD28 pKa = 3.85 TGDD31 pKa = 4.43 DD32 pKa = 3.95 GDD34 pKa = 4.32 GAPAGTDD41 pKa = 2.84 NATGTEE47 pKa = 4.09 PAAGGDD53 pKa = 4.22 DD54 pKa = 3.84 GASDD58 pKa = 3.47 ATFVFGAAGAPTTFDD73 pKa = 3.41 PFYY76 pKa = 10.83 ASDD79 pKa = 3.89 GEE81 pKa = 4.57 TFRR84 pKa = 11.84 VTRR87 pKa = 11.84 QIFQNLIGIEE97 pKa = 4.15 EE98 pKa = 4.7 GGSEE102 pKa = 4.56 AVPEE106 pKa = 4.6 LATSWEE112 pKa = 4.35 SEE114 pKa = 4.48 DD115 pKa = 3.63 GLVWDD120 pKa = 5.03 FAIQEE125 pKa = 4.44 GVTFHH130 pKa = 7.79 DD131 pKa = 4.43 GTPLDD136 pKa = 4.36 AEE138 pKa = 4.32 AVCANFTRR146 pKa = 11.84 WADD149 pKa = 3.53 QNEE152 pKa = 4.38 AGQSPSGAYY161 pKa = 10.26 YY162 pKa = 10.1 YY163 pKa = 11.4 GNDD166 pKa = 3.25 FGFGEE171 pKa = 4.41 DD172 pKa = 4.31 SLYY175 pKa = 10.66 EE176 pKa = 4.13 SCEE179 pKa = 3.95 ATDD182 pKa = 3.74 EE183 pKa = 4.1 LTAQVTVSRR192 pKa = 11.84 VTGKK196 pKa = 10.49 FPMVLSQSSYY206 pKa = 11.1 AIQSPTAMDD215 pKa = 4.01 EE216 pKa = 3.9 YY217 pKa = 10.69 DD218 pKa = 3.55 ANGIALEE225 pKa = 4.46 GEE227 pKa = 3.91 AFVFPEE233 pKa = 4.11 YY234 pKa = 10.63 AQEE237 pKa = 4.44 HH238 pKa = 5.6 PTGTGPFMFEE248 pKa = 4.39 SYY250 pKa = 11.19 DD251 pKa = 3.6 NAGGSVTLTRR261 pKa = 11.84 NDD263 pKa = 4.79 DD264 pKa = 3.57 YY265 pKa = 11.2 WGDD268 pKa = 3.51 PAGVSEE274 pKa = 4.28 IVFRR278 pKa = 11.84 IIPDD282 pKa = 3.23 EE283 pKa = 3.75 NARR286 pKa = 11.84 RR287 pKa = 11.84 QEE289 pKa = 4.13 LEE291 pKa = 3.65 AGTINGYY298 pKa = 9.2 DD299 pKa = 3.71 LPNPVDD305 pKa = 3.37 WQALEE310 pKa = 4.38 DD311 pKa = 4.17 AGNQVLIRR319 pKa = 11.84 DD320 pKa = 4.45 PFNILYY326 pKa = 10.26 LALNPVADD334 pKa = 4.48 PQLEE338 pKa = 4.38 DD339 pKa = 3.62 PLVRR343 pKa = 11.84 QALYY347 pKa = 10.27 HH348 pKa = 6.02 ALNRR352 pKa = 11.84 QQFVTSQLPAGAEE365 pKa = 4.06 VATQFMPSTVSGWSSGIDD383 pKa = 2.99 AYY385 pKa = 10.52 EE386 pKa = 3.93 YY387 pKa = 10.7 DD388 pKa = 3.74 VEE390 pKa = 4.26 KK391 pKa = 11.08 ARR393 pKa = 11.84 DD394 pKa = 3.67 LLEE397 pKa = 4.04 QAGKK401 pKa = 10.5 SDD403 pKa = 3.6 LTIEE407 pKa = 4.21 LWYY410 pKa = 9.4 PSEE413 pKa = 4.0 VTRR416 pKa = 11.84 PYY418 pKa = 10.19 MPDD421 pKa = 2.98 PTRR424 pKa = 11.84 VYY426 pKa = 10.98 DD427 pKa = 3.68 AVKK430 pKa = 10.61 ADD432 pKa = 3.51 WEE434 pKa = 4.27 AAGITVEE441 pKa = 4.5 TVTKK445 pKa = 8.82 PWAGGYY451 pKa = 9.64 IDD453 pKa = 4.97 DD454 pKa = 4.6 TQASRR459 pKa = 11.84 APAFFLGWTGDD470 pKa = 3.82 LNSADD475 pKa = 3.93 NFLCAFFCGDD485 pKa = 3.94 DD486 pKa = 3.78 NQFGTSAYY494 pKa = 9.85 DD495 pKa = 3.08 WHH497 pKa = 7.45 EE498 pKa = 4.35 DD499 pKa = 3.48 LQEE502 pKa = 4.08 QIAAADD508 pKa = 3.82 AEE510 pKa = 4.4 ADD512 pKa = 3.49 EE513 pKa = 4.46 ATRR516 pKa = 11.84 TGLYY520 pKa = 10.06 EE521 pKa = 4.27 SLNEE525 pKa = 4.68 AIMSPEE531 pKa = 4.11 WLPGLPISHH540 pKa = 6.89 SPPAIVVGEE549 pKa = 4.08 NVQGLVASPLTAEE562 pKa = 4.84 DD563 pKa = 4.57 FSTVTITEE571 pKa = 3.98
Molecular weight: 61.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.706
IPC_protein 3.732
Toseland 3.516
ProMoST 3.872
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.63
Rodwell 3.554
Grimsley 3.427
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.554
EMBOSS 3.643
Sillero 3.846
Patrickios 1.405
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.78
Protein with the highest isoelectric point:
>tr|A0A285VUX1|A0A285VUX1_9MICO Lysophospholipase L1 OS=Ornithinimicrobium cerasi OX=2248773 GN=SAMN05421879_11721 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3368
0
3368
1132626
27
2986
336.3
35.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.954 ± 0.057
0.638 ± 0.011
6.374 ± 0.038
6.034 ± 0.039
2.444 ± 0.025
9.65 ± 0.042
2.291 ± 0.02
2.78 ± 0.03
1.356 ± 0.026
10.672 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.805 ± 0.016
1.45 ± 0.024
5.961 ± 0.036
2.868 ± 0.023
8.157 ± 0.046
5.015 ± 0.026
6.135 ± 0.034
9.947 ± 0.047
1.64 ± 0.017
1.828 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here