Amino acid dipepetide frequency for Humitalea rosea

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
25.736AlaAla: 25.736 ± 0.245
1.318AlaCys: 1.318 ± 0.03
6.979AlaAsp: 6.979 ± 0.068
9.503AlaGlu: 9.503 ± 0.102
4.508AlaPhe: 4.508 ± 0.061
14.041AlaGly: 14.041 ± 0.142
2.405AlaHis: 2.405 ± 0.039
6.261AlaIle: 6.261 ± 0.066
2.675AlaLys: 2.675 ± 0.054
17.337AlaLeu: 17.337 ± 0.136
4.558AlaMet: 4.558 ± 0.051
2.475AlaAsn: 2.475 ± 0.043
8.283AlaPro: 8.283 ± 0.11
4.381AlaGln: 4.381 ± 0.054
11.964AlaArg: 11.964 ± 0.11
6.213AlaSer: 6.213 ± 0.064
7.4AlaThr: 7.4 ± 0.072
10.348AlaVal: 10.348 ± 0.083
2.191AlaTrp: 2.191 ± 0.045
2.331AlaTyr: 2.331 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
1.151CysAla: 1.151 ± 0.029
0.115CysCys: 0.115 ± 0.009
0.54CysAsp: 0.54 ± 0.022
0.343CysGlu: 0.343 ± 0.015
0.328CysPhe: 0.328 ± 0.015
1.008CysGly: 1.008 ± 0.026
0.238CysHis: 0.238 ± 0.012
0.371CysIle: 0.371 ± 0.017
0.122CysLys: 0.122 ± 0.009
0.869CysLeu: 0.869 ± 0.025
0.163CysMet: 0.163 ± 0.011
0.186CysAsn: 0.186 ± 0.01
0.492CysPro: 0.492 ± 0.02
0.194CysGln: 0.194 ± 0.009
0.674CysArg: 0.674 ± 0.02
0.36CysSer: 0.36 ± 0.016
0.425CysThr: 0.425 ± 0.018
0.646CysVal: 0.646 ± 0.02
0.137CysTrp: 0.137 ± 0.009
0.167CysTyr: 0.167 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.53AspAla: 7.53 ± 0.076
0.436AspCys: 0.436 ± 0.017
2.205AspAsp: 2.205 ± 0.044
2.408AspGlu: 2.408 ± 0.043
1.727AspPhe: 1.727 ± 0.033
5.064AspGly: 5.064 ± 0.062
1.016AspHis: 1.016 ± 0.026
2.344AspIle: 2.344 ± 0.033
0.822AspLys: 0.822 ± 0.024
5.811AspLeu: 5.811 ± 0.062
1.139AspMet: 1.139 ± 0.028
0.855AspAsn: 0.855 ± 0.03
4.068AspPro: 4.068 ± 0.045
1.359AspGln: 1.359 ± 0.033
4.287AspArg: 4.287 ± 0.059
1.863AspSer: 1.863 ± 0.035
2.361AspThr: 2.361 ± 0.042
3.732AspVal: 3.732 ± 0.056
1.03AspTrp: 1.03 ± 0.03
1.095AspTyr: 1.095 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
9.394GluAla: 9.394 ± 0.104
0.328GluCys: 0.328 ± 0.016
2.459GluAsp: 2.459 ± 0.045
2.661GluGlu: 2.661 ± 0.052
1.435GluPhe: 1.435 ± 0.03
4.41GluGly: 4.41 ± 0.054
1.065GluHis: 1.065 ± 0.03
2.555GluIle: 2.555 ± 0.044
1.098GluLys: 1.098 ± 0.033
4.368GluLeu: 4.368 ± 0.053
1.413GluMet: 1.413 ± 0.028
1.012GluAsn: 1.012 ± 0.025
2.783GluPro: 2.783 ± 0.051
1.741GluGln: 1.741 ± 0.031
4.442GluArg: 4.442 ± 0.061
1.907GluSer: 1.907 ± 0.036
2.988GluThr: 2.988 ± 0.045
4.023GluVal: 4.023 ± 0.055
0.63GluTrp: 0.63 ± 0.021
0.734GluTyr: 0.734 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
4.734PheAla: 4.734 ± 0.057
0.437PheCys: 0.437 ± 0.018
2.188PheAsp: 2.188 ± 0.039
1.552PheGlu: 1.552 ± 0.033
1.085PhePhe: 1.085 ± 0.03
3.698PheGly: 3.698 ± 0.06
0.726PheHis: 0.726 ± 0.022
1.249PheIle: 1.249 ± 0.028
0.496PheLys: 0.496 ± 0.019
3.364PheLeu: 3.364 ± 0.055
0.596PheMet: 0.596 ± 0.02
0.814PheAsn: 0.814 ± 0.022
1.645PhePro: 1.645 ± 0.032
0.884PheGln: 0.884 ± 0.023
2.461PheArg: 2.461 ± 0.039
1.82PheSer: 1.82 ± 0.034
1.873PheThr: 1.873 ± 0.039
2.372PheVal: 2.372 ± 0.039
0.503PheTrp: 0.503 ± 0.019
0.615PheTyr: 0.615 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
11.859GlyAla: 11.859 ± 0.114
0.938GlyCys: 0.938 ± 0.026
4.112GlyAsp: 4.112 ± 0.06
4.416GlyGlu: 4.416 ± 0.06
3.9GlyPhe: 3.9 ± 0.05
8.647GlyGly: 8.647 ± 0.105
1.957GlyHis: 1.957 ± 0.04
4.415GlyIle: 4.415 ± 0.057
2.116GlyLys: 2.116 ± 0.048
10.911GlyLeu: 10.911 ± 0.102
2.711GlyMet: 2.711 ± 0.044
1.875GlyAsn: 1.875 ± 0.045
4.755GlyPro: 4.755 ± 0.064
3.104GlyGln: 3.104 ± 0.043
7.661GlyArg: 7.661 ± 0.072
4.316GlySer: 4.316 ± 0.065
5.03GlyThr: 5.03 ± 0.079
6.876GlyVal: 6.876 ± 0.067
1.765GlyTrp: 1.765 ± 0.035
1.981GlyTyr: 1.981 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
2.792HisAla: 2.792 ± 0.045
0.212HisCys: 0.212 ± 0.011
1.121HisAsp: 1.121 ± 0.026
0.861HisGlu: 0.861 ± 0.024
0.728HisPhe: 0.728 ± 0.024
2.024HisGly: 2.024 ± 0.045
0.507HisHis: 0.507 ± 0.02
0.81HisIle: 0.81 ± 0.024
0.3HisLys: 0.3 ± 0.013
2.2HisLeu: 2.2 ± 0.039
0.429HisMet: 0.429 ± 0.017
0.36HisAsn: 0.36 ± 0.016
1.558HisPro: 1.558 ± 0.037
0.509HisGln: 0.509 ± 0.018
1.513HisArg: 1.513 ± 0.033
0.766HisSer: 0.766 ± 0.024
0.814HisThr: 0.814 ± 0.025
1.478HisVal: 1.478 ± 0.033
0.357HisTrp: 0.357 ± 0.018
0.419HisTyr: 0.419 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
7.51IleAla: 7.51 ± 0.081
0.469IleCys: 0.469 ± 0.018
2.335IleAsp: 2.335 ± 0.037
2.806IleGlu: 2.806 ± 0.041
1.298IlePhe: 1.298 ± 0.033
4.881IleGly: 4.881 ± 0.061
0.796IleHis: 0.796 ± 0.022
1.723IleIle: 1.723 ± 0.037
0.751IleLys: 0.751 ± 0.023
4.117IleLeu: 4.117 ± 0.057
0.745IleMet: 0.745 ± 0.023
0.873IleAsn: 0.873 ± 0.026
2.337IlePro: 2.337 ± 0.042
1.078IleGln: 1.078 ± 0.029
3.141IleArg: 3.141 ± 0.047
2.094IleSer: 2.094 ± 0.041
2.361IleThr: 2.361 ± 0.041
3.21IleVal: 3.21 ± 0.056
0.566IleTrp: 0.566 ± 0.022
0.749IleTyr: 0.749 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
2.538LysAla: 2.538 ± 0.051
0.075LysCys: 0.075 ± 0.007
0.911LysAsp: 0.911 ± 0.028
0.781LysGlu: 0.781 ± 0.025
0.522LysPhe: 0.522 ± 0.021
1.623LysGly: 1.623 ± 0.04
0.37LysHis: 0.37 ± 0.015
0.942LysIle: 0.942 ± 0.027
0.501LysLys: 0.501 ± 0.026
2.021LysLeu: 2.021 ± 0.046
0.432LysMet: 0.432 ± 0.019
0.43LysAsn: 0.43 ± 0.016
1.375LysPro: 1.375 ± 0.036
0.531LysGln: 0.531 ± 0.018
1.564LysArg: 1.564 ± 0.032
0.941LysSer: 0.941 ± 0.027
1.084LysThr: 1.084 ± 0.03
1.485LysVal: 1.485 ± 0.037
0.204LysTrp: 0.204 ± 0.013
0.308LysTyr: 0.308 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
18.052LeuAla: 18.052 ± 0.157
0.992LeuCys: 0.992 ± 0.028
6.274LeuAsp: 6.274 ± 0.07
4.801LeuGlu: 4.801 ± 0.058
3.291LeuPhe: 3.291 ± 0.053
10.007LeuGly: 10.007 ± 0.092
2.257LeuHis: 2.257 ± 0.038
4.108LeuIle: 4.108 ± 0.058
2.029LeuLys: 2.029 ± 0.039
12.312LeuLeu: 12.312 ± 0.128
2.22LeuMet: 2.22 ± 0.046
2.14LeuAsn: 2.14 ± 0.037
7.44LeuPro: 7.44 ± 0.087
2.519LeuGln: 2.519 ± 0.043
9.627LeuArg: 9.627 ± 0.1
5.66LeuSer: 5.66 ± 0.073
5.719LeuThr: 5.719 ± 0.075
8.225LeuVal: 8.225 ± 0.087
1.374LeuTrp: 1.374 ± 0.033
1.668LeuTyr: 1.668 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
3.717MetAla: 3.717 ± 0.051
0.132MetCys: 0.132 ± 0.009
1.178MetAsp: 1.178 ± 0.025
1.039MetGlu: 1.039 ± 0.027
0.601MetPhe: 0.601 ± 0.02
1.991MetGly: 1.991 ± 0.041
0.441MetHis: 0.441 ± 0.02
1.141MetIle: 1.141 ± 0.03
0.6MetLys: 0.6 ± 0.022
2.883MetLeu: 2.883 ± 0.051
0.596MetMet: 0.596 ± 0.02
0.576MetAsn: 0.576 ± 0.02
1.747MetPro: 1.747 ± 0.032
0.776MetGln: 0.776 ± 0.022
1.966MetArg: 1.966 ± 0.036
1.423MetSer: 1.423 ± 0.033
1.734MetThr: 1.734 ± 0.034
1.767MetVal: 1.767 ± 0.036
0.196MetTrp: 0.196 ± 0.011
0.254MetTyr: 0.254 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.705AsnAla: 2.705 ± 0.041
0.181AsnCys: 0.181 ± 0.011
1.008AsnAsp: 1.008 ± 0.032
0.832AsnGlu: 0.832 ± 0.024
0.698AsnPhe: 0.698 ± 0.023
1.809AsnGly: 1.809 ± 0.046
0.435AsnHis: 0.435 ± 0.016
1.002AsnIle: 1.002 ± 0.029
0.364AsnLys: 0.364 ± 0.015
2.169AsnLeu: 2.169 ± 0.037
0.446AsnMet: 0.446 ± 0.018
0.473AsnAsn: 0.473 ± 0.02
1.63AsnPro: 1.63 ± 0.036
0.522AsnGln: 0.522 ± 0.019
1.614AsnArg: 1.614 ± 0.03
0.824AsnSer: 0.824 ± 0.024
1.045AsnThr: 1.045 ± 0.03
1.418AsnVal: 1.418 ± 0.037
0.311AsnTrp: 0.311 ± 0.014
0.425AsnTyr: 0.425 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
9.171ProAla: 9.171 ± 0.117
0.393ProCys: 0.393 ± 0.018
3.918ProAsp: 3.918 ± 0.046
4.191ProGlu: 4.191 ± 0.052
2.0ProPhe: 2.0 ± 0.039
6.612ProGly: 6.612 ± 0.072
1.236ProHis: 1.236 ± 0.033
2.335ProIle: 2.335 ± 0.043
1.087ProLys: 1.087 ± 0.034
5.992ProLeu: 5.992 ± 0.075
1.496ProMet: 1.496 ± 0.035
1.283ProAsn: 1.283 ± 0.031
4.513ProPro: 4.513 ± 0.092
1.656ProGln: 1.656 ± 0.034
4.179ProArg: 4.179 ± 0.058
2.726ProSer: 2.726 ± 0.043
2.742ProThr: 2.742 ± 0.039
4.849ProVal: 4.849 ± 0.066
0.966ProTrp: 0.966 ± 0.029
1.208ProTyr: 1.208 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
4.662GlnAla: 4.662 ± 0.061
0.153GlnCys: 0.153 ± 0.009
1.425GlnAsp: 1.425 ± 0.029
1.238GlnGlu: 1.238 ± 0.028
0.799GlnPhe: 0.799 ± 0.022
2.724GlnGly: 2.724 ± 0.044
0.589GlnHis: 0.589 ± 0.019
1.449GlnIle: 1.449 ± 0.03
0.648GlnLys: 0.648 ± 0.023
2.263GlnLeu: 2.263 ± 0.042
0.824GlnMet: 0.824 ± 0.023
0.592GlnAsn: 0.592 ± 0.021
1.884GlnPro: 1.884 ± 0.036
1.021GlnGln: 1.021 ± 0.034
2.525GlnArg: 2.525 ± 0.051
1.372GlnSer: 1.372 ± 0.029
1.47GlnThr: 1.47 ± 0.028
2.304GlnVal: 2.304 ± 0.041
0.305GlnTrp: 0.305 ± 0.014
0.426GlnTyr: 0.426 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
11.011ArgAla: 11.011 ± 0.109
0.645ArgCys: 0.645 ± 0.02
4.358ArgAsp: 4.358 ± 0.054
3.957ArgGlu: 3.957 ± 0.064
3.057ArgPhe: 3.057 ± 0.05
6.313ArgGly: 6.313 ± 0.07
1.891ArgHis: 1.891 ± 0.039
4.111ArgIle: 4.111 ± 0.053
1.35ArgLys: 1.35 ± 0.034
10.048ArgLeu: 10.048 ± 0.11
2.16ArgMet: 2.16 ± 0.037
1.565ArgAsn: 1.565 ± 0.033
4.735ArgPro: 4.735 ± 0.069
2.61ArgGln: 2.61 ± 0.045
7.202ArgArg: 7.202 ± 0.084
3.339ArgSer: 3.339 ± 0.052
3.29ArgThr: 3.29 ± 0.048
5.629ArgVal: 5.629 ± 0.071
1.158ArgTrp: 1.158 ± 0.026
1.429ArgTyr: 1.429 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
5.929SerAla: 5.929 ± 0.075
0.364SerCys: 0.364 ± 0.016
2.208SerAsp: 2.208 ± 0.044
1.999SerGlu: 1.999 ± 0.038
1.827SerPhe: 1.827 ± 0.036
5.004SerGly: 5.004 ± 0.062
0.888SerHis: 0.888 ± 0.025
2.046SerIle: 2.046 ± 0.039
0.751SerLys: 0.751 ± 0.023
5.345SerLeu: 5.345 ± 0.059
1.085SerMet: 1.085 ± 0.028
0.926SerAsn: 0.926 ± 0.029
2.805SerPro: 2.805 ± 0.042
1.193SerGln: 1.193 ± 0.03
3.461SerArg: 3.461 ± 0.053
1.988SerSer: 1.988 ± 0.041
2.202SerThr: 2.202 ± 0.043
3.356SerVal: 3.356 ± 0.047
0.739SerTrp: 0.739 ± 0.025
0.904SerTyr: 0.904 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
7.122ThrAla: 7.122 ± 0.079
0.373ThrCys: 0.373 ± 0.014
2.616ThrAsp: 2.616 ± 0.048
2.549ThrGlu: 2.549 ± 0.045
1.515ThrPhe: 1.515 ± 0.034
5.13ThrGly: 5.13 ± 0.074
1.025ThrHis: 1.025 ± 0.027
2.44ThrIle: 2.44 ± 0.038
0.935ThrLys: 0.935 ± 0.025
6.196ThrLeu: 6.196 ± 0.068
1.137ThrMet: 1.137 ± 0.027
1.056ThrAsn: 1.056 ± 0.029
3.886ThrPro: 3.886 ± 0.053
1.362ThrGln: 1.362 ± 0.03
3.657ThrArg: 3.657 ± 0.055
2.314ThrSer: 2.314 ± 0.045
2.943ThrThr: 2.943 ± 0.056
3.965ThrVal: 3.965 ± 0.05
0.618ThrTrp: 0.618 ± 0.02
0.94ThrTyr: 0.94 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
11.122ValAla: 11.122 ± 0.101
0.635ValCys: 0.635 ± 0.02
3.444ValAsp: 3.444 ± 0.047
4.132ValGlu: 4.132 ± 0.05
2.658ValPhe: 2.658 ± 0.051
5.439ValGly: 5.439 ± 0.07
1.232ValHis: 1.232 ± 0.027
3.311ValIle: 3.311 ± 0.055
1.412ValLys: 1.412 ± 0.035
8.888ValLeu: 8.888 ± 0.087
1.935ValMet: 1.935 ± 0.04
1.669ValAsn: 1.669 ± 0.037
4.732ValPro: 4.732 ± 0.061
2.143ValGln: 2.143 ± 0.04
4.926ValArg: 4.926 ± 0.061
3.555ValSer: 3.555 ± 0.054
4.661ValThr: 4.661 ± 0.059
6.33ValVal: 6.33 ± 0.085
0.935ValTrp: 0.935 ± 0.025
1.011ValTyr: 1.011 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.645TrpAla: 1.645 ± 0.037
0.146TrpCys: 0.146 ± 0.01
0.712TrpAsp: 0.712 ± 0.021
0.573TrpGlu: 0.573 ± 0.02
0.529TrpPhe: 0.529 ± 0.017
1.103TrpGly: 1.103 ± 0.027
0.36TrpHis: 0.36 ± 0.016
0.601TrpIle: 0.601 ± 0.018
0.291TrpLys: 0.291 ± 0.014
1.881TrpLeu: 1.881 ± 0.035
0.36TrpMet: 0.36 ± 0.019
0.375TrpAsn: 0.375 ± 0.017
0.899TrpPro: 0.899 ± 0.023
0.605TrpGln: 0.605 ± 0.02
1.528TrpArg: 1.528 ± 0.034
0.751TrpSer: 0.751 ± 0.024
0.747TrpThr: 0.747 ± 0.027
0.92TrpVal: 0.92 ± 0.026
0.256TrpTrp: 0.256 ± 0.015
0.251TrpTyr: 0.251 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.526TyrAla: 2.526 ± 0.037
0.188TyrCys: 0.188 ± 0.011
1.162TyrAsp: 1.162 ± 0.029
0.802TyrGlu: 0.802 ± 0.024
0.607TyrPhe: 0.607 ± 0.02
1.816TyrGly: 1.816 ± 0.043
0.324TyrHis: 0.324 ± 0.014
0.626TyrIle: 0.626 ± 0.02
0.289TyrLys: 0.289 ± 0.014
1.738TyrLeu: 1.738 ± 0.034
0.313TyrMet: 0.313 ± 0.016
0.377TyrAsn: 0.377 ± 0.015
0.988TyrPro: 0.988 ± 0.03
0.486TyrGln: 0.486 ± 0.018
1.465TyrArg: 1.465 ± 0.035
0.788TyrSer: 0.788 ± 0.023
0.89TyrThr: 0.89 ± 0.027
1.236TyrVal: 1.236 ± 0.028
0.296TyrTrp: 0.296 ± 0.014
0.389TyrTyr: 0.389 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4650 proteins (1537676 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski