Lolium latent virus (isolate Lolium/USA/US1/-) (LoLV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Lolavirus; Lolium latent virus

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B1PS80|CAPSD_LOLV Capsid protein OS=Lolium latent virus (isolate Lolium/USA/US1/-) OX=686945 GN=ORF5 PE=1 SV=1
MM1 pKa = 7.38SLSFSLIVFAVGVAVSIGVLTLTTQQSSSYY31 pKa = 10.83CLILVDD37 pKa = 3.63GAKK40 pKa = 10.29AVVEE44 pKa = 4.71GCHH47 pKa = 6.17LRR49 pKa = 11.84QDD51 pKa = 3.29IPAILSEE58 pKa = 4.78LKK60 pKa = 10.15PASSPFNPLFCSS72 pKa = 3.59

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B1PS81|ORF6_LOLV Uncharacterized ORF6 protein OS=Lolium latent virus (isolate Lolium/USA/US1/-) OX=686945 GN=ORF6 PE=4 SV=1
MM1 pKa = 7.64APPTRR6 pKa = 11.84EE7 pKa = 3.8YY8 pKa = 10.67RR9 pKa = 11.84CTPNYY14 pKa = 10.17HH15 pKa = 5.9SARR18 pKa = 11.84HH19 pKa = 4.99QMSSLLGLCKK29 pKa = 10.42GGVGPQPRR37 pKa = 11.84PWCEE41 pKa = 3.36KK42 pKa = 10.12TMVV45 pKa = 3.31

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2527

45

1729

421.2

47.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.627 ± 1.014

1.662 ± 0.725

4.828 ± 0.751

6.055 ± 0.848

3.68 ± 0.312

5.144 ± 0.524

3.285 ± 0.36

5.065 ± 0.372

4.749 ± 0.751

8.666 ± 0.684

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.555

3.918 ± 0.495

6.727 ± 0.66

4.867 ± 0.511

5.778 ± 0.744

6.767 ± 1.188

7.281 ± 0.355

5.382 ± 0.593

1.306 ± 0.137

3.918 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski