Amino acid dipepetide frequency for Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.973AlaAla: 5.973 ± 0.032	1.2AlaCys: 1.2 ± 0.011	2.958AlaAsp: 2.958 ± 0.016	4.207AlaGlu: 4.207 ± 0.025	2.695AlaPhe: 2.695 ± 0.018	3.898AlaGly: 3.898 ± 0.019	1.194AlaHis: 1.194 ± 0.011	3.543AlaIle: 3.543 ± 0.022	3.996AlaLys: 3.996 ± 0.022	6.3AlaLeu: 6.3 ± 0.029	1.807AlaMet: 1.807 ± 0.013	2.447AlaAsn: 2.447 ± 0.015	2.645AlaPro: 2.645 ± 0.018	1.937AlaGln: 1.937 ± 0.015	3.327AlaArg: 3.327 ± 0.018	5.92AlaSer: 5.92 ± 0.025	3.6AlaThr: 3.6 ± 0.019	4.711AlaVal: 4.711 ± 0.022	0.712AlaTrp: 0.712 ± 0.009	1.755AlaTyr: 1.755 ± 0.013	0.0AlaXaa: 0.0 ± 0.0
Cys	0.941CysAla: 0.941 ± 0.009	0.527CysCys: 0.527 ± 0.008	0.939CysAsp: 0.939 ± 0.01	0.932CysGlu: 0.932 ± 0.01	0.959CysPhe: 0.959 ± 0.01	1.404CysGly: 1.404 ± 0.014	0.444CysHis: 0.444 ± 0.007	0.973CysIle: 0.973 ± 0.01	1.152CysLys: 1.152 ± 0.012	1.9CysLeu: 1.9 ± 0.013	0.399CysMet: 0.399 ± 0.006	0.824CysAsn: 0.824 ± 0.009	0.879CysPro: 0.879 ± 0.011	0.541CysGln: 0.541 ± 0.007	1.068CysArg: 1.068 ± 0.01	1.785CysSer: 1.785 ± 0.014	0.812CysThr: 0.812 ± 0.008	1.192CysVal: 1.192 ± 0.011	0.23CysTrp: 0.23 ± 0.005	0.573CysTyr: 0.573 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	3.34AspAla: 3.34 ± 0.018	0.948AspCys: 0.948 ± 0.01	3.833AspAsp: 3.833 ± 0.027	4.242AspGlu: 4.242 ± 0.022	2.431AspPhe: 2.431 ± 0.015	3.774AspGly: 3.774 ± 0.02	1.294AspHis: 1.294 ± 0.011	2.931AspIle: 2.931 ± 0.015	2.815AspLys: 2.815 ± 0.02	5.268AspLeu: 5.268 ± 0.024	1.312AspMet: 1.312 ± 0.012	2.027AspAsn: 2.027 ± 0.015	2.702AspPro: 2.702 ± 0.017	1.79AspGln: 1.79 ± 0.014	2.445AspArg: 2.445 ± 0.017	4.449AspSer: 4.449 ± 0.024	2.219AspThr: 2.219 ± 0.015	3.736AspVal: 3.736 ± 0.018	0.692AspTrp: 0.692 ± 0.009	1.612AspTyr: 1.612 ± 0.013	0.0AspXaa: 0.0 ± 0.0
Glu	4.889GluAla: 4.889 ± 0.025	0.877GluCys: 0.877 ± 0.009	4.256GluAsp: 4.256 ± 0.027	7.096GluGlu: 7.096 ± 0.043	2.475GluPhe: 2.475 ± 0.015	3.487GluGly: 3.487 ± 0.017	1.205GluHis: 1.205 ± 0.011	4.065GluIle: 4.065 ± 0.02	5.255GluLys: 5.255 ± 0.029	5.783GluLeu: 5.783 ± 0.027	1.866GluMet: 1.866 ± 0.015	3.175GluAsn: 3.175 ± 0.018	2.246GluPro: 2.246 ± 0.017	2.102GluGln: 2.102 ± 0.015	3.551GluArg: 3.551 ± 0.022	5.003GluSer: 5.003 ± 0.027	3.71GluThr: 3.71 ± 0.022	4.038GluVal: 4.038 ± 0.022	0.753GluTrp: 0.753 ± 0.008	1.662GluTyr: 1.662 ± 0.014	0.0GluXaa: 0.0 ± 0.0
Phe	2.535PheAla: 2.535 ± 0.018	0.917PheCys: 0.917 ± 0.009	2.449PheAsp: 2.449 ± 0.015	2.363PheGlu: 2.363 ± 0.015	2.047PhePhe: 2.047 ± 0.017	3.066PheGly: 3.066 ± 0.019	1.112PheHis: 1.112 ± 0.01	2.041PheIle: 2.041 ± 0.013	2.279PheLys: 2.279 ± 0.015	4.438PheLeu: 4.438 ± 0.021	0.997PheMet: 0.997 ± 0.01	1.702PheAsn: 1.702 ± 0.012	2.127PhePro: 2.127 ± 0.014	1.507PheGln: 1.507 ± 0.011	2.168PheArg: 2.168 ± 0.014	4.185PheSer: 4.185 ± 0.021	2.154PheThr: 2.154 ± 0.015	2.882PheVal: 2.882 ± 0.018	0.566PheTrp: 0.566 ± 0.008	1.28PheTyr: 1.28 ± 0.011	0.0PheXaa: 0.0 ± 0.0
Gly	3.577GlyAla: 3.577 ± 0.02	1.238GlyCys: 1.238 ± 0.012	3.514GlyAsp: 3.514 ± 0.017	3.872GlyGlu: 3.872 ± 0.02	3.343GlyPhe: 3.343 ± 0.02	5.352GlyGly: 5.352 ± 0.036	1.422GlyHis: 1.422 ± 0.014	3.434GlyIle: 3.434 ± 0.018	4.226GlyLys: 4.226 ± 0.019	5.736GlyLeu: 5.736 ± 0.024	1.427GlyMet: 1.427 ± 0.013	3.06GlyAsn: 3.06 ± 0.021	2.245GlyPro: 2.245 ± 0.013	1.954GlyGln: 1.954 ± 0.014	3.503GlyArg: 3.503 ± 0.019	5.859GlySer: 5.859 ± 0.028	3.126GlyThr: 3.126 ± 0.017	4.146GlyVal: 4.146 ± 0.019	0.847GlyTrp: 0.847 ± 0.01	2.132GlyTyr: 2.132 ± 0.014	0.0GlyXaa: 0.0 ± 0.0
His	1.223HisAla: 1.223 ± 0.01	0.506HisCys: 0.506 ± 0.007	1.113HisAsp: 1.113 ± 0.012	1.298HisGlu: 1.298 ± 0.011	1.0HisPhe: 1.0 ± 0.009	1.632HisGly: 1.632 ± 0.013	0.993HisHis: 0.993 ± 0.014	1.158HisIle: 1.158 ± 0.01	1.195HisLys: 1.195 ± 0.011	2.247HisLeu: 2.247 ± 0.015	0.562HisMet: 0.562 ± 0.007	0.946HisAsn: 0.946 ± 0.009	1.252HisPro: 1.252 ± 0.012	1.019HisGln: 1.019 ± 0.011	1.403HisArg: 1.403 ± 0.012	1.789HisSer: 1.789 ± 0.013	0.951HisThr: 0.951 ± 0.009	1.484HisVal: 1.484 ± 0.012	0.291HisTrp: 0.291 ± 0.006	0.696HisTyr: 0.696 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	3.448IleAla: 3.448 ± 0.017	1.126IleCys: 1.126 ± 0.009	2.963IleAsp: 2.963 ± 0.018	3.201IleGlu: 3.201 ± 0.019	2.245IlePhe: 2.245 ± 0.015	3.371IleGly: 3.371 ± 0.017	1.274IleHis: 1.274 ± 0.011	2.644IleIle: 2.644 ± 0.016	2.959IleLys: 2.959 ± 0.017	5.026IleLeu: 5.026 ± 0.025	1.101IleMet: 1.101 ± 0.011	2.162IleAsn: 2.162 ± 0.015	2.874IlePro: 2.874 ± 0.022	1.906IleGln: 1.906 ± 0.012	2.745IleArg: 2.745 ± 0.018	4.951IleSer: 4.951 ± 0.024	2.69IleThr: 2.69 ± 0.016	3.501IleVal: 3.501 ± 0.018	0.697IleTrp: 0.697 ± 0.008	1.54IleTyr: 1.54 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	4.044LysAla: 4.044 ± 0.02	0.982LysCys: 0.982 ± 0.01	3.295LysAsp: 3.295 ± 0.02	4.854LysGlu: 4.854 ± 0.029	2.181LysPhe: 2.181 ± 0.014	3.519LysGly: 3.519 ± 0.02	1.345LysHis: 1.345 ± 0.013	3.44LysIle: 3.44 ± 0.019	5.375LysLys: 5.375 ± 0.035	6.112LysLeu: 6.112 ± 0.029	1.596LysMet: 1.596 ± 0.014	2.799LysAsn: 2.799 ± 0.018	3.026LysPro: 3.026 ± 0.018	2.319LysGln: 2.319 ± 0.016	3.918LysArg: 3.918 ± 0.023	4.957LysSer: 4.957 ± 0.021	3.434LysThr: 3.434 ± 0.021	3.76LysVal: 3.76 ± 0.019	0.816LysTrp: 0.816 ± 0.009	1.583LysTyr: 1.583 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	6.253LeuAla: 6.253 ± 0.025	1.881LeuCys: 1.881 ± 0.014	5.03LeuAsp: 5.03 ± 0.024	6.259LeuGlu: 6.259 ± 0.031	3.875LeuPhe: 3.875 ± 0.023	5.649LeuGly: 5.649 ± 0.026	2.407LeuHis: 2.407 ± 0.015	4.626LeuIle: 4.626 ± 0.025	6.233LeuLys: 6.233 ± 0.026	9.619LeuLeu: 9.619 ± 0.046	2.241LeuMet: 2.241 ± 0.013	3.781LeuAsn: 3.781 ± 0.019	4.953LeuPro: 4.953 ± 0.02	3.88LeuGln: 3.88 ± 0.02	5.54LeuArg: 5.54 ± 0.025	8.567LeuSer: 8.567 ± 0.039	4.552LeuThr: 4.552 ± 0.022	6.45LeuVal: 6.45 ± 0.027	1.125LeuTrp: 1.125 ± 0.011	2.496LeuTyr: 2.496 ± 0.015	0.0LeuXaa: 0.0 ± 0.0
Met	2.07MetAla: 2.07 ± 0.013	0.332MetCys: 0.332 ± 0.005	1.41MetAsp: 1.41 ± 0.011	2.042MetGlu: 2.042 ± 0.015	0.912MetPhe: 0.912 ± 0.01	1.523MetGly: 1.523 ± 0.012	0.468MetHis: 0.468 ± 0.006	1.338MetIle: 1.338 ± 0.012	1.701MetLys: 1.701 ± 0.012	2.086MetLeu: 2.086 ± 0.014	0.768MetMet: 0.768 ± 0.01	1.07MetAsn: 1.07 ± 0.01	0.954MetPro: 0.954 ± 0.01	0.824MetGln: 0.824 ± 0.009	1.272MetArg: 1.272 ± 0.012	1.906MetSer: 1.906 ± 0.013	1.099MetThr: 1.099 ± 0.011	1.734MetVal: 1.734 ± 0.014	0.27MetTrp: 0.27 ± 0.005	0.613MetTyr: 0.613 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	2.561AsnAla: 2.561 ± 0.018	0.818AsnCys: 0.818 ± 0.011	2.035AsnAsp: 2.035 ± 0.013	2.471AsnGlu: 2.471 ± 0.017	1.75AsnPhe: 1.75 ± 0.013	3.234AsnGly: 3.234 ± 0.019	1.141AsnHis: 1.141 ± 0.012	2.353AsnIle: 2.353 ± 0.015	2.422AsnLys: 2.422 ± 0.015	4.455AsnLeu: 4.455 ± 0.028	1.082AsnMet: 1.082 ± 0.01	2.337AsnAsn: 2.337 ± 0.022	2.458AsnPro: 2.458 ± 0.016	1.714AsnGln: 1.714 ± 0.013	2.208AsnArg: 2.208 ± 0.015	3.708AsnSer: 3.708 ± 0.02	2.038AsnThr: 2.038 ± 0.014	2.831AsnVal: 2.831 ± 0.015	0.549AsnTrp: 0.549 ± 0.007	1.214AsnTyr: 1.214 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	2.828ProAla: 2.828 ± 0.015	0.762ProCys: 0.762 ± 0.009	2.479ProAsp: 2.479 ± 0.018	3.412ProGlu: 3.412 ± 0.018	1.969ProPhe: 1.969 ± 0.013	2.663ProGly: 2.663 ± 0.017	1.017ProHis: 1.017 ± 0.009	2.259ProIle: 2.259 ± 0.013	2.828ProLys: 2.828 ± 0.016	4.204ProLeu: 4.204 ± 0.02	1.002ProMet: 1.002 ± 0.01	2.204ProAsn: 2.204 ± 0.015	3.791ProPro: 3.791 ± 0.044	1.747ProGln: 1.747 ± 0.018	2.566ProArg: 2.566 ± 0.015	4.987ProSer: 4.987 ± 0.024	2.48ProThr: 2.48 ± 0.015	3.212ProVal: 3.212 ± 0.019	0.607ProTrp: 0.607 ± 0.007	1.319ProTyr: 1.319 ± 0.013	0.0ProXaa: 0.0 ± 0.0
Gln	2.229GlnAla: 2.229 ± 0.014	0.55GlnCys: 0.55 ± 0.007	1.648GlnAsp: 1.648 ± 0.012	2.471GlnGlu: 2.471 ± 0.017	1.308GlnPhe: 1.308 ± 0.01	1.988GlnGly: 1.988 ± 0.015	0.819GlnHis: 0.819 ± 0.009	1.97GlnIle: 1.97 ± 0.015	2.254GlnLys: 2.254 ± 0.015	3.196GlnLeu: 3.196 ± 0.018	0.942GlnMet: 0.942 ± 0.011	1.684GlnAsn: 1.684 ± 0.013	1.657GlnPro: 1.657 ± 0.015	1.953GlnGln: 1.953 ± 0.028	2.156GlnArg: 2.156 ± 0.015	2.796GlnSer: 2.796 ± 0.018	1.818GlnThr: 1.818 ± 0.012	2.203GlnVal: 2.203 ± 0.013	0.435GlnTrp: 0.435 ± 0.005	0.882GlnTyr: 0.882 ± 0.01	0.0GlnXaa: 0.0 ± 0.0
Arg	3.15ArgAla: 3.15 ± 0.018	1.065ArgCys: 1.065 ± 0.01	2.862ArgAsp: 2.862 ± 0.018	3.613ArgGlu: 3.613 ± 0.021	2.605ArgPhe: 2.605 ± 0.016	3.277ArgGly: 3.277 ± 0.021	1.231ArgHis: 1.231 ± 0.01	2.961ArgIle: 2.961 ± 0.019	3.918ArgLys: 3.918 ± 0.021	5.186ArgLeu: 5.186 ± 0.02	1.283ArgMet: 1.283 ± 0.01	2.552ArgAsn: 2.552 ± 0.014	2.339ArgPro: 2.339 ± 0.015	1.826ArgGln: 1.826 ± 0.015	4.267ArgArg: 4.267 ± 0.032	4.72ArgSer: 4.72 ± 0.027	2.468ArgThr: 2.468 ± 0.014	3.52ArgVal: 3.52 ± 0.019	0.762ArgTrp: 0.762 ± 0.009	1.531ArgTyr: 1.531 ± 0.014	0.0ArgXaa: 0.0 ± 0.0
Ser	5.216SerAla: 5.216 ± 0.024	1.733SerCys: 1.733 ± 0.014	4.676SerAsp: 4.676 ± 0.019	5.073SerGlu: 5.073 ± 0.026	4.144SerPhe: 4.144 ± 0.021	6.077SerGly: 6.077 ± 0.028	1.991SerHis: 1.991 ± 0.014	4.34SerIle: 4.34 ± 0.021	5.087SerLys: 5.087 ± 0.023	8.97SerLeu: 8.97 ± 0.034	2.066SerMet: 2.066 ± 0.014	3.881SerAsn: 3.881 ± 0.02	4.762SerPro: 4.762 ± 0.032	2.96SerGln: 2.96 ± 0.019	4.802SerArg: 4.802 ± 0.026	11.822SerSer: 11.822 ± 0.064	4.468SerThr: 4.468 ± 0.021	5.618SerVal: 5.618 ± 0.023	1.165SerTrp: 1.165 ± 0.01	2.404SerTyr: 2.404 ± 0.016	0.0SerXaa: 0.0 ± 0.0
Thr	3.351ThrAla: 3.351 ± 0.019	0.972ThrCys: 0.972 ± 0.01	2.365ThrAsp: 2.365 ± 0.015	3.134ThrGlu: 3.134 ± 0.018	2.076ThrPhe: 2.076 ± 0.014	3.439ThrGly: 3.439 ± 0.019	1.018ThrHis: 1.018 ± 0.01	2.69ThrIle: 2.69 ± 0.015	2.99ThrLys: 2.99 ± 0.018	4.715ThrLeu: 4.715 ± 0.02	1.224ThrMet: 1.224 ± 0.01	2.114ThrAsn: 2.114 ± 0.015	2.545ThrPro: 2.545 ± 0.017	1.588ThrGln: 1.588 ± 0.013	2.612ThrArg: 2.612 ± 0.014	4.64ThrSer: 4.64 ± 0.021	3.139ThrThr: 3.139 ± 0.018	3.574ThrVal: 3.574 ± 0.021	0.661ThrTrp: 0.661 ± 0.008	1.396ThrTyr: 1.396 ± 0.011	0.0ThrXaa: 0.0 ± 0.0
Val	4.752ValAla: 4.752 ± 0.019	1.192ValCys: 1.192 ± 0.01	3.761ValAsp: 3.761 ± 0.021	4.517ValGlu: 4.517 ± 0.022	2.929ValPhe: 2.929 ± 0.019	3.82ValGly: 3.82 ± 0.021	1.425ValHis: 1.425 ± 0.01	3.462ValIle: 3.462 ± 0.017	4.157ValLys: 4.157 ± 0.021	6.279ValLeu: 6.279 ± 0.027	1.643ValMet: 1.643 ± 0.013	2.643ValAsn: 2.643 ± 0.016	3.135ValPro: 3.135 ± 0.018	2.065ValGln: 2.065 ± 0.014	3.205ValArg: 3.205 ± 0.017	5.904ValSer: 5.904 ± 0.026	3.481ValThr: 3.481 ± 0.018	4.991ValVal: 4.991 ± 0.024	0.799ValTrp: 0.799 ± 0.008	2.029ValTyr: 2.029 ± 0.015	0.0ValXaa: 0.0 ± 0.0
Trp	0.704TrpAla: 0.704 ± 0.009	0.247TrpCys: 0.247 ± 0.005	0.701TrpAsp: 0.701 ± 0.008	0.777TrpGlu: 0.777 ± 0.009	0.571TrpPhe: 0.571 ± 0.008	0.717TrpGly: 0.717 ± 0.009	0.255TrpHis: 0.255 ± 0.004	0.733TrpIle: 0.733 ± 0.008	0.921TrpLys: 0.921 ± 0.009	1.172TrpLeu: 1.172 ± 0.012	0.331TrpMet: 0.331 ± 0.005	0.679TrpAsn: 0.679 ± 0.009	0.473TrpPro: 0.473 ± 0.006	0.403TrpGln: 0.403 ± 0.006	0.881TrpArg: 0.881 ± 0.009	1.021TrpSer: 1.021 ± 0.012	0.65TrpThr: 0.65 ± 0.008	0.784TrpVal: 0.784 ± 0.009	0.229TrpTrp: 0.229 ± 0.005	0.352TrpTyr: 0.352 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.739TyrAla: 1.739 ± 0.014	0.6TyrCys: 0.6 ± 0.008	1.573TyrAsp: 1.573 ± 0.013	1.66TyrGlu: 1.66 ± 0.013	1.301TyrPhe: 1.301 ± 0.013	2.121TyrGly: 2.121 ± 0.015	0.701TyrHis: 0.701 ± 0.007	1.464TyrIle: 1.464 ± 0.012	1.596TyrLys: 1.596 ± 0.013	2.722TyrLeu: 2.722 ± 0.017	0.746TyrMet: 0.746 ± 0.008	1.293TyrAsn: 1.293 ± 0.011	1.272TyrPro: 1.272 ± 0.013	0.923TyrGln: 0.923 ± 0.009	1.498TyrArg: 1.498 ± 0.011	2.288TyrSer: 2.288 ± 0.015	1.355TyrThr: 1.355 ± 0.011	1.82TyrVal: 1.82 ± 0.013	0.398TyrTrp: 0.398 ± 0.007	1.004TyrTyr: 1.004 ± 0.01	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 28349 proteins (11549942 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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