Aeromicrobium erythreum
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3120 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U4CNL5|A0A0U4CNL5_9ACTN DNA helicase OS=Aeromicrobium erythreum OX=2041 GN=AERYTH_05905 PE=4 SV=1
MM1 pKa = 7.63 ARR3 pKa = 11.84 FICPNCEE10 pKa = 3.74 YY11 pKa = 10.94 VYY13 pKa = 11.0 DD14 pKa = 4.2 EE15 pKa = 4.71 AVGDD19 pKa = 4.13 PRR21 pKa = 11.84 EE22 pKa = 4.12 GWPAGTAFSDD32 pKa = 4.05 VDD34 pKa = 4.37 PDD36 pKa = 3.76 WTCPDD41 pKa = 3.57 CGVRR45 pKa = 11.84 EE46 pKa = 4.12 QVDD49 pKa = 4.12 FVPEE53 pKa = 3.94 AEE55 pKa = 4.39 FTHH58 pKa = 6.66 NDD60 pKa = 2.91 QDD62 pKa = 3.62 GDD64 pKa = 4.0 IISAEE69 pKa = 3.87 TRR71 pKa = 11.84 AAQARR76 pKa = 11.84 QAEE79 pKa = 4.43 QGAQQQ84 pKa = 3.21
Molecular weight: 9.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.592
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.91
Patrickios 1.875
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A0U4D6Q5|A0A0U4D6Q5_9ACTN LuxR family transcriptional regulator OS=Aeromicrobium erythreum OX=2041 GN=AERYTH_03820 PE=4 SV=1
MM1 pKa = 7.74 SKK3 pKa = 8.93 RR4 pKa = 11.84 TYY6 pKa = 10.0 QPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.97 KK16 pKa = 9.33 HH17 pKa = 4.25 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILANRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.78 GRR40 pKa = 11.84 ANLSAA45 pKa = 4.66
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3120
0
3120
981426
37
3574
314.6
33.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.997 ± 0.061
0.659 ± 0.01
6.847 ± 0.044
5.649 ± 0.043
2.728 ± 0.025
9.089 ± 0.046
2.22 ± 0.021
2.973 ± 0.038
1.834 ± 0.035
10.458 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.652 ± 0.019
1.543 ± 0.023
5.463 ± 0.031
2.812 ± 0.022
7.924 ± 0.043
5.241 ± 0.026
6.18 ± 0.04
10.377 ± 0.047
1.496 ± 0.018
1.858 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here