Pseudomassariella vexata
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12557 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y2DR73|A0A1Y2DR73_9PEZI Ketoacyl-synt_C domain-containing protein (Fragment) OS=Pseudomassariella vexata OX=1141098 GN=BCR38DRAFT_347936 PE=4 SV=1
MM1 pKa = 7.28 PTLAPEE7 pKa = 4.29 LTKK10 pKa = 10.7 DD11 pKa = 3.86 APDD14 pKa = 3.5 LAKK17 pKa = 10.47 RR18 pKa = 11.84 AACTFSGADD27 pKa = 3.89 GYY29 pKa = 11.93 ASASKK34 pKa = 10.69 SKK36 pKa = 10.64 ADD38 pKa = 3.51 CATIVLSALTVPSGVTLDD56 pKa = 4.05 LTDD59 pKa = 4.84 LSDD62 pKa = 3.6 GTLVTFEE69 pKa = 5.81 GEE71 pKa = 4.51 TTWEE75 pKa = 4.09 YY76 pKa = 11.34 EE77 pKa = 3.98 EE78 pKa = 4.54 WEE80 pKa = 4.74 GPLFAVSGTKK90 pKa = 8.58 ITVDD94 pKa = 3.33 GASGHH99 pKa = 5.65 SLNGNGAKK107 pKa = 8.91 WWDD110 pKa = 3.57 GEE112 pKa = 4.23 GDD114 pKa = 3.75 SGVTKK119 pKa = 10.47 PKK121 pKa = 9.94 MFQAHH126 pKa = 6.69 DD127 pKa = 5.16 LIDD130 pKa = 3.66 STIQNINIVNSPVQVFSINGCDD152 pKa = 3.27 TLAVNSVTIDD162 pKa = 3.42 NSDD165 pKa = 3.57 GDD167 pKa = 4.53 DD168 pKa = 4.38 DD169 pKa = 5.19 SLGHH173 pKa = 5.98 NTDD176 pKa = 3.85 CFDD179 pKa = 3.72 IGSSTGVTITDD190 pKa = 3.72 ATCYY194 pKa = 10.48 NQDD197 pKa = 3.22 DD198 pKa = 4.24 CVAINSGTDD207 pKa = 2.85 ITFTGGYY214 pKa = 9.4 CSGGHH219 pKa = 5.94 GLSIGSIGGRR229 pKa = 11.84 DD230 pKa = 3.89 DD231 pKa = 3.75 NTVKK235 pKa = 10.08 TVTFSDD241 pKa = 3.54 SKK243 pKa = 10.9 IINSQNGVRR252 pKa = 11.84 IKK254 pKa = 9.89 TKK256 pKa = 10.59 SGDD259 pKa = 3.27 TGTVEE264 pKa = 5.21 DD265 pKa = 3.62 ITYY268 pKa = 10.57 RR269 pKa = 11.84 DD270 pKa = 3.36 ITLSGITDD278 pKa = 3.37 YY279 pKa = 11.64 GITVDD284 pKa = 4.03 QAYY287 pKa = 10.59 DD288 pKa = 3.54 GDD290 pKa = 4.4 DD291 pKa = 3.46 ATDD294 pKa = 5.28 GITISGFTLDD304 pKa = 4.56 GVTGTTDD311 pKa = 2.62 SDD313 pKa = 3.28 ADD315 pKa = 3.49 YY316 pKa = 10.93 AIYY319 pKa = 10.41 VNCGSDD325 pKa = 3.94 SCTDD329 pKa = 3.4 WTWTDD334 pKa = 3.4 VSVTGADD341 pKa = 3.62 NSCSNQPSGITCC353 pKa = 4.45
Molecular weight: 36.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.49
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.719
Rodwell 3.554
Grimsley 3.389
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.19
Thurlkill 3.554
EMBOSS 3.732
Sillero 3.859
Patrickios 0.846
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A1Y2DFQ1|A0A1Y2DFQ1_9PEZI HET domain-containing protein OS=Pseudomassariella vexata OX=1141098 GN=BCR38DRAFT_448054 PE=4 SV=1
MM1 pKa = 7.32 KK2 pKa = 9.55 TRR4 pKa = 11.84 KK5 pKa = 9.43 KK6 pKa = 10.55 SSPLPVSRR14 pKa = 11.84 PRR16 pKa = 11.84 QHH18 pKa = 6.69 LAGPSRR24 pKa = 11.84 SPITAPPTKK33 pKa = 9.38 TRR35 pKa = 11.84 KK36 pKa = 9.6 KK37 pKa = 10.26 SLPLPVSSPLATPNLASPKK56 pKa = 10.25
Molecular weight: 5.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.41
IPC2_protein 10.906
IPC_protein 12.442
Toseland 12.632
ProMoST 13.1
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.086
Rodwell 12.544
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.115
Sillero 12.632
Patrickios 12.281
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.991
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12557
0
12557
5490982
49
4995
437.3
48.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.612 ± 0.02
1.312 ± 0.009
5.66 ± 0.016
5.968 ± 0.021
3.765 ± 0.013
7.075 ± 0.02
2.383 ± 0.009
4.93 ± 0.014
4.872 ± 0.02
8.804 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.32 ± 0.007
3.772 ± 0.012
5.911 ± 0.024
3.927 ± 0.014
5.942 ± 0.019
8.099 ± 0.027
6.054 ± 0.016
6.262 ± 0.015
1.529 ± 0.009
2.805 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here