Pseudomassariella vexata

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Pseudomassariaceae; Pseudomassariella

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12557 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2DR73|A0A1Y2DR73_9PEZI Ketoacyl-synt_C domain-containing protein (Fragment) OS=Pseudomassariella vexata OX=1141098 GN=BCR38DRAFT_347936 PE=4 SV=1
MM1 pKa = 7.28PTLAPEE7 pKa = 4.29LTKK10 pKa = 10.7DD11 pKa = 3.86APDD14 pKa = 3.5LAKK17 pKa = 10.47RR18 pKa = 11.84AACTFSGADD27 pKa = 3.89GYY29 pKa = 11.93ASASKK34 pKa = 10.69SKK36 pKa = 10.64ADD38 pKa = 3.51CATIVLSALTVPSGVTLDD56 pKa = 4.05LTDD59 pKa = 4.84LSDD62 pKa = 3.6GTLVTFEE69 pKa = 5.81GEE71 pKa = 4.51TTWEE75 pKa = 4.09YY76 pKa = 11.34EE77 pKa = 3.98EE78 pKa = 4.54WEE80 pKa = 4.74GPLFAVSGTKK90 pKa = 8.58ITVDD94 pKa = 3.33GASGHH99 pKa = 5.65SLNGNGAKK107 pKa = 8.91WWDD110 pKa = 3.57GEE112 pKa = 4.23GDD114 pKa = 3.75SGVTKK119 pKa = 10.47PKK121 pKa = 9.94MFQAHH126 pKa = 6.69DD127 pKa = 5.16LIDD130 pKa = 3.66STIQNINIVNSPVQVFSINGCDD152 pKa = 3.27TLAVNSVTIDD162 pKa = 3.42NSDD165 pKa = 3.57GDD167 pKa = 4.53DD168 pKa = 4.38DD169 pKa = 5.19SLGHH173 pKa = 5.98NTDD176 pKa = 3.85CFDD179 pKa = 3.72IGSSTGVTITDD190 pKa = 3.72ATCYY194 pKa = 10.48NQDD197 pKa = 3.22DD198 pKa = 4.24CVAINSGTDD207 pKa = 2.85ITFTGGYY214 pKa = 9.4CSGGHH219 pKa = 5.94GLSIGSIGGRR229 pKa = 11.84DD230 pKa = 3.89DD231 pKa = 3.75NTVKK235 pKa = 10.08TVTFSDD241 pKa = 3.54SKK243 pKa = 10.9IINSQNGVRR252 pKa = 11.84IKK254 pKa = 9.89TKK256 pKa = 10.59SGDD259 pKa = 3.27TGTVEE264 pKa = 5.21DD265 pKa = 3.62ITYY268 pKa = 10.57RR269 pKa = 11.84DD270 pKa = 3.36ITLSGITDD278 pKa = 3.37YY279 pKa = 11.64GITVDD284 pKa = 4.03QAYY287 pKa = 10.59DD288 pKa = 3.54GDD290 pKa = 4.4DD291 pKa = 3.46ATDD294 pKa = 5.28GITISGFTLDD304 pKa = 4.56GVTGTTDD311 pKa = 2.62SDD313 pKa = 3.28ADD315 pKa = 3.49YY316 pKa = 10.93AIYY319 pKa = 10.41VNCGSDD325 pKa = 3.94SCTDD329 pKa = 3.4WTWTDD334 pKa = 3.4VSVTGADD341 pKa = 3.62NSCSNQPSGITCC353 pKa = 4.45

Molecular weight: 36.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2DFQ1|A0A1Y2DFQ1_9PEZI HET domain-containing protein OS=Pseudomassariella vexata OX=1141098 GN=BCR38DRAFT_448054 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.55TRR4 pKa = 11.84KK5 pKa = 9.43KK6 pKa = 10.55SSPLPVSRR14 pKa = 11.84PRR16 pKa = 11.84QHH18 pKa = 6.69LAGPSRR24 pKa = 11.84SPITAPPTKK33 pKa = 9.38TRR35 pKa = 11.84KK36 pKa = 9.6KK37 pKa = 10.26SLPLPVSSPLATPNLASPKK56 pKa = 10.25

Molecular weight: 5.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12557

0

12557

5490982

49

4995

437.3

48.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.612 ± 0.02

1.312 ± 0.009

5.66 ± 0.016

5.968 ± 0.021

3.765 ± 0.013

7.075 ± 0.02

2.383 ± 0.009

4.93 ± 0.014

4.872 ± 0.02

8.804 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.32 ± 0.007

3.772 ± 0.012

5.911 ± 0.024

3.927 ± 0.014

5.942 ± 0.019

8.099 ± 0.027

6.054 ± 0.016

6.262 ± 0.015

1.529 ± 0.009

2.805 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski