Allisonella histaminiformans
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1536 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G5VYP5|A0A1G5VYP5_9FIRM Predicted arabinose efflux permease MFS family OS=Allisonella histaminiformans OX=209880 GN=SAMN02910343_01005 PE=4 SV=1
MM1 pKa = 7.43 SFIMLVPFLGLLVIIVIWDD20 pKa = 3.52 KK21 pKa = 11.53 LEE23 pKa = 4.86 SIPSVRR29 pKa = 11.84 KK30 pKa = 9.52 GKK32 pKa = 10.01 HH33 pKa = 5.24 SLWFPPPTPGSQFPVSTTAKK53 pKa = 9.45 EE54 pKa = 4.16 ISNHH58 pKa = 5.41 SSPPEE63 pKa = 3.68 PLFSVSDD70 pKa = 3.77 KK71 pKa = 10.98 QPLIRR76 pKa = 11.84 IHH78 pKa = 6.26 SVHH81 pKa = 6.85 PSHH84 pKa = 7.33 RR85 pKa = 11.84 YY86 pKa = 8.43 HH87 pKa = 6.58 GHH89 pKa = 5.65 VGCDD93 pKa = 2.96 TDD95 pKa = 6.22 DD96 pKa = 3.95 EE97 pKa = 4.87 NEE99 pKa = 4.23 HH100 pKa = 6.27 NTTQDD105 pKa = 3.3 YY106 pKa = 10.82 EE107 pKa = 4.41 YY108 pKa = 10.96 DD109 pKa = 4.4 DD110 pKa = 4.27 YY111 pKa = 12.02 DD112 pKa = 4.46 SYY114 pKa = 12.09 DD115 pKa = 3.54 GYY117 pKa = 11.49 SYY119 pKa = 10.87 IWGYY123 pKa = 11.68 DD124 pKa = 2.96 NGFADD129 pKa = 4.34 GTDD132 pKa = 3.22 SAEE135 pKa = 4.09 RR136 pKa = 11.84 DD137 pKa = 3.3 IEE139 pKa = 4.01 EE140 pKa = 4.15 ARR142 pKa = 11.84 QFGYY146 pKa = 10.55 EE147 pKa = 3.89 QGFEE151 pKa = 4.41 DD152 pKa = 4.96 GNASAYY158 pKa = 10.71 DD159 pKa = 3.89 DD160 pKa = 5.63 DD161 pKa = 7.61 DD162 pKa = 6.25 YY163 pKa = 12.11 DD164 pKa = 6.4 DD165 pKa = 4.98 YY166 pKa = 12.18 DD167 pKa = 4.54 NDD169 pKa = 3.57 YY170 pKa = 11.59 DD171 pKa = 6.24 DD172 pKa = 5.78 YY173 pKa = 11.98 DD174 pKa = 6.15 DD175 pKa = 5.6 YY176 pKa = 12.18 DD177 pKa = 6.19 DD178 pKa = 5.7 YY179 pKa = 12.14 DD180 pKa = 5.82 DD181 pKa = 5.41 DD182 pKa = 4.25
Molecular weight: 20.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.617
ProMoST 3.999
Dawson 3.859
Bjellqvist 4.037
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.528
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.681
EMBOSS 3.821
Sillero 3.973
Patrickios 0.502
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.886
Protein with the highest isoelectric point:
>tr|A0A1G5W4W1|A0A1G5W4W1_9FIRM DNA ligase OS=Allisonella histaminiformans OX=209880 GN=ligA PE=3 SV=1
MM1 pKa = 7.75 AKK3 pKa = 10.54 GKK5 pKa = 7.67 MTYY8 pKa = 9.74 QPNNLWRR15 pKa = 11.84 KK16 pKa = 6.7 RR17 pKa = 11.84 THH19 pKa = 6.17 GFRR22 pKa = 11.84 ARR24 pKa = 11.84 MKK26 pKa = 8.64 TKK28 pKa = 10.33 AGRR31 pKa = 11.84 IVLKK35 pKa = 10.36 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 9.89 GRR42 pKa = 11.84 KK43 pKa = 8.67 VLSAA47 pKa = 4.05
Molecular weight: 5.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1536
0
1536
476354
13
1544
310.1
34.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.478 ± 0.061
1.235 ± 0.024
5.453 ± 0.047
6.637 ± 0.072
3.989 ± 0.038
7.577 ± 0.063
2.409 ± 0.029
6.9 ± 0.049
6.008 ± 0.05
9.065 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.282 ± 0.029
3.795 ± 0.041
3.844 ± 0.037
3.259 ± 0.042
5.273 ± 0.052
5.764 ± 0.043
5.29 ± 0.04
7.125 ± 0.051
1.062 ± 0.022
3.555 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here