Enterobacter phage Arya
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A193GYD1|A0A193GYD1_9CAUD Putative tail protein OS=Enterobacter phage Arya OX=1864622 GN=BI096_gp14 PE=4 SV=1
MM1 pKa = 7.72 AAQTYY6 pKa = 6.7 VTSDD10 pKa = 3.37 GDD12 pKa = 3.81 TADD15 pKa = 3.97 YY16 pKa = 10.59 IAWKK20 pKa = 10.49 YY21 pKa = 10.78 YY22 pKa = 10.19 GNQDD26 pKa = 3.55 PGTVEE31 pKa = 4.29 ALVDD35 pKa = 4.08 ANKK38 pKa = 10.57 GLADD42 pKa = 4.43 IGPLLPAGMVITLPEE57 pKa = 4.02 ISTPATQQGIKK68 pKa = 10.22 LWDD71 pKa = 3.31
Molecular weight: 7.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.808
IPC_protein 3.694
Toseland 3.49
ProMoST 3.795
Dawson 3.732
Bjellqvist 3.986
Wikipedia 3.732
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.834
Patrickios 1.901
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|A0A193GYR8|A0A193GYR8_9CAUD Uncharacterized protein OS=Enterobacter phage Arya OX=1864622 GN=BI096_gp44 PE=4 SV=1
MM1 pKa = 6.53 TTFAINFIAVCFIFIWASWCVMYY24 pKa = 10.88 SGVKK28 pKa = 10.28 DD29 pKa = 4.12 GIVGKK34 pKa = 10.49 LFFSLVAISALAIIIHH50 pKa = 6.26 TLTGHH55 pKa = 4.97 YY56 pKa = 10.13 AKK58 pKa = 10.59 RR59 pKa = 11.84 PFITMNVSIALVGARR74 pKa = 11.84 HH75 pKa = 6.12 VFLSWLKK82 pKa = 10.34 RR83 pKa = 11.84 FKK85 pKa = 10.54 AAQQRR90 pKa = 11.84 HH91 pKa = 5.13 HH92 pKa = 6.44 SS93 pKa = 3.77
Molecular weight: 10.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.794
IPC_protein 10.175
Toseland 10.628
ProMoST 10.204
Dawson 10.745
Bjellqvist 10.394
Wikipedia 10.891
Rodwell 11.228
Grimsley 10.789
Solomon 10.804
Lehninger 10.774
Nozaki 10.628
DTASelect 10.379
Thurlkill 10.628
EMBOSS 11.008
Sillero 10.657
Patrickios 11.023
IPC_peptide 10.804
IPC2_peptide 9.428
IPC2.peptide.svr19 8.455
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61
0
61
12980
27
813
212.8
23.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.048 ± 0.373
1.133 ± 0.141
6.333 ± 0.265
5.77 ± 0.372
3.451 ± 0.23
8.544 ± 0.433
1.579 ± 0.152
5.27 ± 0.209
4.954 ± 0.335
7.612 ± 0.232
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.427 ± 0.173
4.445 ± 0.222
4.561 ± 0.25
4.099 ± 0.248
5.763 ± 0.36
5.524 ± 0.279
6.425 ± 0.445
6.672 ± 0.323
1.81 ± 0.166
2.581 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here