Mesobacillus foraminis
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5324 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R2BNC1|A0A4R2BNC1_9BACI Uncharacterized protein OS=Mesobacillus foraminis OX=279826 GN=EV146_101373 PE=4 SV=1
MM1 pKa = 7.31 PAIVGAVQVISIGSSGVFNIGDD23 pKa = 3.75 VYY25 pKa = 11.19 KK26 pKa = 10.73 IMPVSTAKK34 pKa = 9.76 TFSGAGSFNTGDD46 pKa = 3.48 SMQVYY51 pKa = 10.26 NNQSSTNTFDD61 pKa = 4.23 QDD63 pKa = 4.44 SVDD66 pKa = 3.18 QGNYY70 pKa = 9.16 FNAA73 pKa = 4.19
Molecular weight: 7.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.935
IPC_protein 3.745
Toseland 3.528
ProMoST 4.012
Dawson 3.795
Bjellqvist 3.973
Wikipedia 3.859
Rodwell 3.592
Grimsley 3.465
Solomon 3.745
Lehninger 3.694
Nozaki 3.961
DTASelect 4.253
Thurlkill 3.668
EMBOSS 3.846
Sillero 3.884
Patrickios 1.952
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|A0A4R2B0E7|A0A4R2B0E7_9BACI TetR family transcriptional regulator OS=Mesobacillus foraminis OX=279826 GN=EV146_11567 PE=4 SV=1
MM1 pKa = 7.24 KK2 pKa = 10.34 RR3 pKa = 11.84 SFAKK7 pKa = 9.9 SWSSFQGNSISHH19 pKa = 6.53 KK20 pKa = 10.67 KK21 pKa = 10.25 SDD23 pKa = 3.66 ACTGIASVCRR33 pKa = 11.84 QKK35 pKa = 11.04 RR36 pKa = 11.84 FGIVSFRR43 pKa = 11.84 TPFLILLCFYY53 pKa = 11.24 LLYY56 pKa = 10.43 NDD58 pKa = 3.33 PHH60 pKa = 6.38 FRR62 pKa = 11.84 LLGHH66 pKa = 6.7 NSTSPCPIGHH76 pKa = 7.81 LFQVQGSEE84 pKa = 4.33 SKK86 pKa = 10.59 HH87 pKa = 5.39 RR88 pKa = 11.84 LHH90 pKa = 6.77 RR91 pKa = 11.84 QYY93 pKa = 11.08 FLNHH97 pKa = 5.81 LRR99 pKa = 11.84 VVQCMPCFSFASANIRR115 pKa = 11.84 SVVSFRR121 pKa = 11.84 FEE123 pKa = 3.7 KK124 pKa = 10.88 CFLLLVYY131 pKa = 10.72 ASGGRR136 pKa = 11.84 LLFLKK141 pKa = 10.53 ASSSPKK147 pKa = 10.13 RR148 pKa = 11.84 FIFPFF153 pKa = 3.98
Molecular weight: 17.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.663
IPC_protein 10.116
Toseland 10.555
ProMoST 10.175
Dawson 10.672
Bjellqvist 10.35
Wikipedia 10.833
Rodwell 11.038
Grimsley 10.716
Solomon 10.73
Lehninger 10.701
Nozaki 10.584
DTASelect 10.335
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.599
Patrickios 10.774
IPC_peptide 10.73
IPC2_peptide 9.619
IPC2.peptide.svr19 8.455
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5324
0
5324
1559474
29
4227
292.9
32.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.286 ± 0.033
0.726 ± 0.011
5.061 ± 0.025
7.5 ± 0.038
4.573 ± 0.026
7.334 ± 0.028
2.07 ± 0.017
7.434 ± 0.034
6.747 ± 0.032
9.845 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.748 ± 0.017
4.347 ± 0.023
3.806 ± 0.028
3.56 ± 0.02
4.16 ± 0.026
6.151 ± 0.022
5.195 ± 0.023
6.787 ± 0.03
1.119 ± 0.015
3.55 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here