Streptococcus phage Javan549

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AMQ6|A0A4D6AMQ6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan549 OX=2548252 GN=Javan549_0041 PE=4 SV=1
MM1 pKa = 6.96TVKK4 pKa = 10.46EE5 pKa = 5.07LINEE9 pKa = 4.01LQMYY13 pKa = 10.47DD14 pKa = 3.44EE15 pKa = 5.14DD16 pKa = 5.01KK17 pKa = 11.27EE18 pKa = 4.59VVLTIANVYY27 pKa = 9.23PVLHH31 pKa = 6.47EE32 pKa = 4.5LVDD35 pKa = 4.12LEE37 pKa = 4.5TGLVRR42 pKa = 11.84LSSDD46 pKa = 3.45CQIGFEE52 pKa = 5.02FNFLSDD58 pKa = 3.38NRR60 pKa = 11.84LEE62 pKa = 5.04IEE64 pKa = 4.58GVWW67 pKa = 3.21

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AI31|A0A4D6AI31_9CAUD Uncharacterized protein OS=Streptococcus phage Javan549 OX=2548252 GN=Javan549_0034 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.02RR4 pKa = 11.84IKK6 pKa = 9.94KK7 pKa = 9.82KK8 pKa = 9.35RR9 pKa = 11.84WLEE12 pKa = 3.76IKK14 pKa = 10.29LAEE17 pKa = 4.34CLARR21 pKa = 11.84EE22 pKa = 4.43HH23 pKa = 7.19LLMSAVTDD31 pKa = 3.66QNDD34 pKa = 3.89KK35 pKa = 10.59IVRR38 pKa = 11.84QAKK41 pKa = 9.91EE42 pKa = 3.68ITDD45 pKa = 3.44LRR47 pKa = 11.84LIIEE51 pKa = 4.55RR52 pKa = 11.84NAQATNSRR60 pKa = 11.84FDD62 pKa = 3.46KK63 pKa = 11.08LEE65 pKa = 4.01KK66 pKa = 10.23QVANSNTKK74 pKa = 10.25KK75 pKa = 10.26SWFGRR80 pKa = 11.84KK81 pKa = 7.94

Molecular weight: 9.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

11302

42

1800

182.3

20.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.406 ± 0.713

0.557 ± 0.136

6.079 ± 0.266

7.91 ± 0.449

3.566 ± 0.268

5.725 ± 0.278

1.336 ± 0.182

6.371 ± 0.26

8.353 ± 0.432

8.777 ± 0.375

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.274

5.663 ± 0.384

2.699 ± 0.256

4.017 ± 0.208

4.265 ± 0.233

6.22 ± 0.507

6.937 ± 0.599

6.663 ± 0.249

1.177 ± 0.11

3.813 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski