Trichoderma harzianum CBS 226.95

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma; Trichoderma harzianum

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14049 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4AT64|A0A2T4AT64_TRIHA Uncharacterized protein OS=Trichoderma harzianum CBS 226.95 OX=983964 GN=M431DRAFT_488926 PE=3 SV=1
MM1 pKa = 7.57KK2 pKa = 10.43LLAITALFIAGALAVPAGSYY22 pKa = 9.13PPPPPPDD29 pKa = 3.3YY30 pKa = 10.97GGYY33 pKa = 9.98SPVPSSSGDD42 pKa = 3.3DD43 pKa = 3.53YY44 pKa = 11.57GYY46 pKa = 11.1VPPTPTPPPPPPPPPPPPSYY66 pKa = 11.09DD67 pKa = 3.28DD68 pKa = 4.21GGDD71 pKa = 3.66YY72 pKa = 10.52EE73 pKa = 5.1PPTDD77 pKa = 4.19GDD79 pKa = 4.1GDD81 pKa = 4.11SSDD84 pKa = 4.55GGDD87 pKa = 5.18DD88 pKa = 5.3GDD90 pKa = 5.61DD91 pKa = 4.02SDD93 pKa = 5.8DD94 pKa = 4.58GSDD97 pKa = 3.51GGDD100 pKa = 3.5GEE102 pKa = 4.76RR103 pKa = 11.84SQTNLCPAVISGIAKK118 pKa = 9.52CCSTNVLDD126 pKa = 6.37LLNLDD131 pKa = 3.86CVSPNKK137 pKa = 9.84EE138 pKa = 3.8PKK140 pKa = 9.73DD141 pKa = 3.55RR142 pKa = 11.84KK143 pKa = 10.41DD144 pKa = 3.31FEE146 pKa = 5.22KK147 pKa = 10.54IFAGMGRR154 pKa = 11.84QATCCTLGLLGLGLLCGHH172 pKa = 7.56

Molecular weight: 17.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4A7B2|A0A2T4A7B2_TRIHA MFS domain-containing protein OS=Trichoderma harzianum CBS 226.95 OX=983964 GN=M431DRAFT_497253 PE=4 SV=1
MM1 pKa = 7.97PFTTHH6 pKa = 6.61RR7 pKa = 11.84RR8 pKa = 11.84HH9 pKa = 5.76HH10 pKa = 6.31HH11 pKa = 4.84TTTTTTARR19 pKa = 11.84PYY21 pKa = 10.08RR22 pKa = 11.84RR23 pKa = 11.84SIFSRR28 pKa = 11.84RR29 pKa = 11.84TPRR32 pKa = 11.84TPRR35 pKa = 11.84VHH37 pKa = 6.38HH38 pKa = 5.48QRR40 pKa = 11.84KK41 pKa = 6.91PTLKK45 pKa = 10.45DD46 pKa = 3.28KK47 pKa = 11.53VSGALLKK54 pKa = 10.95LRR56 pKa = 11.84GSLTRR61 pKa = 11.84RR62 pKa = 11.84PGVKK66 pKa = 9.89AAGTRR71 pKa = 11.84RR72 pKa = 11.84MRR74 pKa = 11.84GTDD77 pKa = 2.81GRR79 pKa = 11.84GSHH82 pKa = 4.94NRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84FLL89 pKa = 3.8

Molecular weight: 10.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14048

1

14049

6187755

49

20877

440.4

48.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.625 ± 0.018

1.345 ± 0.009

5.709 ± 0.015

6.035 ± 0.02

3.838 ± 0.012

6.761 ± 0.024

2.404 ± 0.009

5.214 ± 0.018

4.878 ± 0.019

9.037 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.008

3.739 ± 0.011

5.806 ± 0.024

4.041 ± 0.016

5.91 ± 0.019

8.221 ± 0.025

5.812 ± 0.013

6.071 ± 0.016

1.525 ± 0.008

2.807 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski