Saccharomonospora xinjiangensis XJ-54
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4307 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I0V7R3|I0V7R3_9PSEU Transcriptional regulator OS=Saccharomonospora xinjiangensis XJ-54 OX=882086 GN=SacxiDRAFT_3975 PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 10.58 VSVDD6 pKa = 3.69 QDD8 pKa = 3.51 ICCGAGQCALLAPEE22 pKa = 5.12 VFDD25 pKa = 5.32 QRR27 pKa = 11.84 DD28 pKa = 3.29 EE29 pKa = 4.88 DD30 pKa = 4.39 GVVVLLDD37 pKa = 3.82 PEE39 pKa = 4.62 PGEE42 pKa = 4.05 EE43 pKa = 3.98 SHH45 pKa = 7.17 AVTRR49 pKa = 11.84 EE50 pKa = 3.83 AADD53 pKa = 3.83 GCPSGAIVVAEE64 pKa = 3.99 NN65 pKa = 3.24
Molecular weight: 6.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.821
IPC_protein 3.719
Toseland 3.541
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.012
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|I0V2I2|I0V2I2_9PSEU UPF0301 protein SacxiDRAFT_2103 OS=Saccharomonospora xinjiangensis XJ-54 OX=882086 GN=SacxiDRAFT_2103 PE=3 SV=1
MM1 pKa = 7.53 SKK3 pKa = 10.53 GKK5 pKa = 8.66 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 THH19 pKa = 5.69 GFRR22 pKa = 11.84 LRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AILAARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.28 GRR42 pKa = 11.84 NRR44 pKa = 11.84 LSAA47 pKa = 3.84
Molecular weight: 5.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.374
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.735
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.457
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.285
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4307
0
4307
1425417
24
5972
331.0
35.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.105 ± 0.061
0.762 ± 0.01
6.199 ± 0.035
6.101 ± 0.034
2.777 ± 0.02
9.263 ± 0.04
2.265 ± 0.018
3.18 ± 0.026
1.894 ± 0.027
10.381 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.652 ± 0.017
1.741 ± 0.018
5.789 ± 0.03
2.539 ± 0.021
8.242 ± 0.044
5.404 ± 0.025
6.049 ± 0.026
9.31 ± 0.041
1.451 ± 0.015
1.898 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here