Solibacillus silvestris (strain StLB046) (Bacillus silvestris)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Solibacillus; Solibacillus silvestris

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3812 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2F0N8|F2F0N8_SOLSS Permease OS=Solibacillus silvestris (strain StLB046) OX=1002809 GN=SSIL_1859 PE=3 SV=1
MM1 pKa = 7.45FIKK4 pKa = 10.58YY5 pKa = 10.04DD6 pKa = 3.77EE7 pKa = 4.6YY8 pKa = 11.72DD9 pKa = 3.65LLEE12 pKa = 4.59LFQSEE17 pKa = 4.49PVSISGNIDD26 pKa = 3.53DD27 pKa = 5.52GEE29 pKa = 4.53LIYY32 pKa = 10.11TYY34 pKa = 11.22KK35 pKa = 10.66DD36 pKa = 3.28DD37 pKa = 4.3QNFKK41 pKa = 11.14VILTT45 pKa = 3.87

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2F280|F2F280_SOLSS Succinate dehydrogenase/fumarate reductase flavoprotein subunit OS=Solibacillus silvestris (strain StLB046) OX=1002809 GN=SSIL_0909 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.08NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.36KK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3812

0

3812

1086794

39

2312

285.1

31.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.522 ± 0.041

0.669 ± 0.013

4.959 ± 0.027

7.63 ± 0.05

4.628 ± 0.034

6.607 ± 0.043

2.015 ± 0.02

8.102 ± 0.035

6.542 ± 0.036

9.635 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.83 ± 0.019

4.615 ± 0.026

3.46 ± 0.024

3.956 ± 0.028

3.785 ± 0.028

5.718 ± 0.027

5.709 ± 0.028

7.089 ± 0.032

0.93 ± 0.013

3.597 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski