Alteromonas confluentis
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4086 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E7ZF31|A0A1E7ZF31_9ALTE 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase OS=Alteromonas confluentis OX=1656094 GN=BFC18_04945 PE=3 SV=1
MM1 pKa = 7.69 KK2 pKa = 10.5 APIVLPLVLGITASVSASGSDD23 pKa = 3.59 LLISQYY29 pKa = 11.15 VEE31 pKa = 4.04 GSSSNKK37 pKa = 9.88 ALEE40 pKa = 4.7 LYY42 pKa = 10.78 NPTTEE47 pKa = 4.27 SVSLSGYY54 pKa = 8.36 TISVYY59 pKa = 11.31 NNGGLSASSIVDD71 pKa = 3.53 LSGSIAPGGTLVIAQSGAEE90 pKa = 4.13 DD91 pKa = 3.77 ALKK94 pKa = 10.86 ALADD98 pKa = 3.82 EE99 pKa = 4.87 LTTQSLYY106 pKa = 11.27 NGDD109 pKa = 3.88 DD110 pKa = 4.28 AIILTDD116 pKa = 3.73 DD117 pKa = 4.28 AGNILDD123 pKa = 5.06 SIGQLGVDD131 pKa = 4.49 PGSSWSEE138 pKa = 3.81 GGLSTKK144 pKa = 10.57 DD145 pKa = 2.83 QTLIRR150 pKa = 11.84 NSSVTTGDD158 pKa = 3.45 TNPNDD163 pKa = 3.69 AYY165 pKa = 10.87 GLGEE169 pKa = 4.38 WVGLPQNDD177 pKa = 3.62 YY178 pKa = 10.45 TGLGSHH184 pKa = 7.49 DD185 pKa = 4.09 YY186 pKa = 11.28 DD187 pKa = 5.43 GGGDD191 pKa = 3.68 GDD193 pKa = 4.03 GGGGEE198 pKa = 4.89 DD199 pKa = 3.89 EE200 pKa = 5.25 DD201 pKa = 5.32 LGGVCTNCPDD211 pKa = 3.39 IDD213 pKa = 3.99 KK214 pKa = 11.12 VADD217 pKa = 3.64 ASTFDD222 pKa = 3.67 PAVYY226 pKa = 9.63 YY227 pKa = 10.75 AAVQSEE233 pKa = 4.13 IDD235 pKa = 3.79 AGSDD239 pKa = 3.13 TAIIRR244 pKa = 11.84 QILSEE249 pKa = 4.48 TISGQHH255 pKa = 5.38 VLTYY259 pKa = 10.62 SEE261 pKa = 4.2 VWTALTEE268 pKa = 4.18 TDD270 pKa = 4.33 EE271 pKa = 6.11 DD272 pKa = 4.22 PANPDD277 pKa = 3.95 NILLIYY283 pKa = 9.96 SGNSIPKK290 pKa = 9.33 SEE292 pKa = 4.51 NGSGSDD298 pKa = 4.58 SSQPDD303 pKa = 3.24 YY304 pKa = 11.05 WNRR307 pKa = 11.84 EE308 pKa = 3.91 HH309 pKa = 6.94 SWPKK313 pKa = 9.64 SHH315 pKa = 6.39 GFSSSSAEE323 pKa = 3.9 AYY325 pKa = 9.68 TDD327 pKa = 3.47 IQHH330 pKa = 6.86 LRR332 pKa = 11.84 PSDD335 pKa = 3.13 ITVNSSRR342 pKa = 11.84 GNLDD346 pKa = 3.0 FDD348 pKa = 4.67 YY349 pKa = 11.32 SDD351 pKa = 4.35 APLPEE356 pKa = 5.25 APANRR361 pKa = 11.84 RR362 pKa = 11.84 DD363 pKa = 3.56 SDD365 pKa = 3.82 SFEE368 pKa = 4.21 PRR370 pKa = 11.84 DD371 pKa = 3.67 AVKK374 pKa = 10.95 GDD376 pKa = 3.42 IARR379 pKa = 11.84 QMLYY383 pKa = 9.72 MDD385 pKa = 4.14 IRR387 pKa = 11.84 YY388 pKa = 9.12 EE389 pKa = 4.15 GAGSDD394 pKa = 3.85 VTPDD398 pKa = 3.2 LALVNRR404 pKa = 11.84 ITSSGEE410 pKa = 3.36 PSLGKK415 pKa = 10.51 LCTLLAWNDD424 pKa = 3.65 ADD426 pKa = 3.64 PVDD429 pKa = 3.92 VVEE432 pKa = 4.84 TNRR435 pKa = 11.84 QSAIYY440 pKa = 9.11 EE441 pKa = 4.04 YY442 pKa = 10.54 QGNRR446 pKa = 11.84 NPFVDD451 pKa = 3.48 HH452 pKa = 7.04 PEE454 pKa = 4.07 WVSLFYY460 pKa = 11.14 SADD463 pKa = 3.27 SCDD466 pKa = 3.53 AVEE469 pKa = 5.1 PEE471 pKa = 4.41 PTPDD475 pKa = 4.09 PEE477 pKa = 4.65 PEE479 pKa = 4.41 PEE481 pKa = 4.25 PEE483 pKa = 4.03 PTPVPGGFSPLTLTGVFDD501 pKa = 4.58 GPLSGGTPKK510 pKa = 10.8 GIEE513 pKa = 3.82 LFVNQNIDD521 pKa = 3.59 DD522 pKa = 4.71 LSTCGVGSANNGGGTDD538 pKa = 3.7 GEE540 pKa = 4.72 EE541 pKa = 4.04 FTFPAVSAVAGQFIYY556 pKa = 10.28 IASEE560 pKa = 4.05 SAGFEE565 pKa = 4.2 AFFGFAPDD573 pKa = 3.75 YY574 pKa = 10.48 TSGAMSINGDD584 pKa = 3.5 DD585 pKa = 4.63 AIEE588 pKa = 4.22 VFCDD592 pKa = 3.9 GEE594 pKa = 4.61 VVDD597 pKa = 6.54 LYY599 pKa = 11.8 GDD601 pKa = 3.64 INTDD605 pKa = 3.17 GTGEE609 pKa = 4.02 AWDD612 pKa = 4.95 HH613 pKa = 6.18 LDD615 pKa = 2.82 SWAYY619 pKa = 10.25 RR620 pKa = 11.84 IAGGPSATFDD630 pKa = 3.4 VANWEE635 pKa = 4.31 VAGTNVFDD643 pKa = 5.33 GEE645 pKa = 4.55 TSNATAAVPFPIGTYY660 pKa = 7.05 VTPATEE666 pKa = 4.58 LFFSEE671 pKa = 4.68 YY672 pKa = 10.22 IEE674 pKa = 4.62 GSSNNKK680 pKa = 9.6 ALEE683 pKa = 4.08 IVNLTSAPVDD693 pKa = 3.77 LSAYY697 pKa = 9.22 DD698 pKa = 3.34 VQMFINGRR706 pKa = 11.84 DD707 pKa = 3.57 YY708 pKa = 11.48 AGQTFTLSGTLAPGDD723 pKa = 3.88 VYY725 pKa = 11.71 VLANSGSDD733 pKa = 3.48 SAILAVADD741 pKa = 4.06 LTSGSLTFNGDD752 pKa = 3.32 DD753 pKa = 5.18 AIVLRR758 pKa = 11.84 NNGEE762 pKa = 4.03 IVDD765 pKa = 3.81 SFGRR769 pKa = 11.84 IGEE772 pKa = 4.43 DD773 pKa = 3.44 PGSYY777 pKa = 8.58 WQGDD781 pKa = 3.67 DD782 pKa = 4.3 VRR784 pKa = 11.84 TQNRR788 pKa = 11.84 TLVRR792 pKa = 11.84 NAAITEE798 pKa = 4.29 GDD800 pKa = 3.44 TEE802 pKa = 5.05 AYY804 pKa = 10.56 DD805 pKa = 3.76 EE806 pKa = 4.96 FDD808 pKa = 5.28 PSLEE812 pKa = 3.83 WDD814 pKa = 4.19 VYY816 pKa = 11.47 DD817 pKa = 4.04 QDD819 pKa = 3.79 TFQFLGAYY827 pKa = 9.27 GSSDD831 pKa = 2.95 GGGGDD836 pKa = 3.93 EE837 pKa = 5.4 IGVCGDD843 pKa = 3.15 EE844 pKa = 3.94 ATFISEE850 pKa = 4.34 VQGSGAEE857 pKa = 4.15 SPLAGQSVVIEE868 pKa = 4.61 AIVTHH873 pKa = 5.55 VTPALEE879 pKa = 4.28 GFWVQEE885 pKa = 3.9 EE886 pKa = 4.34 AADD889 pKa = 4.35 SDD891 pKa = 4.5 GDD893 pKa = 3.85 DD894 pKa = 3.7 TTSEE898 pKa = 4.52 GVFIASTSLIEE909 pKa = 4.3 VVTEE913 pKa = 3.88 GDD915 pKa = 3.93 VIRR918 pKa = 11.84 VAGNVTEE925 pKa = 4.93 DD926 pKa = 3.33 YY927 pKa = 11.4 GRR929 pKa = 11.84 TLVEE933 pKa = 4.05 ATSDD937 pKa = 3.57 ALICGTGAVTPVVISLPMEE956 pKa = 4.28 SADD959 pKa = 4.04 SFEE962 pKa = 4.96 KK963 pKa = 10.6 YY964 pKa = 10.13 EE965 pKa = 4.19 GMVVTSSANWTISNIYY981 pKa = 9.03 PYY983 pKa = 11.05 SDD985 pKa = 3.72 FGEE988 pKa = 4.33 VWVSTEE994 pKa = 3.74 RR995 pKa = 11.84 LFNPTQIHH1003 pKa = 6.2 LPGTEE1008 pKa = 3.77 ASAALAASNALDD1020 pKa = 4.24 VLIIDD1025 pKa = 5.04 DD1026 pKa = 4.36 NADD1029 pKa = 3.48 GYY1031 pKa = 11.25 ASEE1034 pKa = 4.08 WMLPEE1039 pKa = 5.54 GGFNPDD1045 pKa = 2.65 NTIRR1049 pKa = 11.84 SGDD1052 pKa = 4.36 LITGVTGVMDD1062 pKa = 3.99 YY1063 pKa = 10.99 GYY1065 pKa = 9.72 SAYY1068 pKa = 10.32 RR1069 pKa = 11.84 IRR1071 pKa = 11.84 PTEE1074 pKa = 4.01 VPSLVAEE1081 pKa = 4.78 NYY1083 pKa = 9.73 RR1084 pKa = 11.84 EE1085 pKa = 3.83 SSPYY1089 pKa = 10.03 VEE1091 pKa = 4.44 EE1092 pKa = 5.02 GNLKK1096 pKa = 9.6 VASFNVLNLFNGDD1109 pKa = 3.35 GNGEE1113 pKa = 4.21 GFPTARR1119 pKa = 11.84 GADD1122 pKa = 3.4 SLSEE1126 pKa = 4.16 YY1127 pKa = 10.13 EE1128 pKa = 4.12 RR1129 pKa = 11.84 QLAKK1133 pKa = 10.1 IVNALVEE1140 pKa = 3.98 IDD1142 pKa = 4.03 ADD1144 pKa = 3.87 VLGLMEE1150 pKa = 5.01 IEE1152 pKa = 4.21 NDD1154 pKa = 3.52 GFGPLSAVAQLTEE1167 pKa = 3.9 ALNDD1171 pKa = 3.49 EE1172 pKa = 4.79 FGEE1175 pKa = 4.42 GTYY1178 pKa = 10.37 AYY1180 pKa = 10.28 VDD1182 pKa = 3.22 AGGDD1186 pKa = 3.51 QLGTDD1191 pKa = 5.06 AITSGILYY1199 pKa = 9.62 RR1200 pKa = 11.84 PAVVTPVGSPAILLEE1215 pKa = 4.41 ANSIVDD1221 pKa = 3.43 EE1222 pKa = 4.51 EE1223 pKa = 5.07 GPLFVDD1229 pKa = 3.57 YY1230 pKa = 11.24 GNRR1233 pKa = 11.84 PSLSQLFTHH1242 pKa = 6.2 VEE1244 pKa = 3.98 SGKK1247 pKa = 8.57 TFAVDD1252 pKa = 3.5 VNHH1255 pKa = 6.89 LKK1257 pKa = 10.89 SKK1259 pKa = 10.5 GSSSSCQEE1267 pKa = 4.89 DD1268 pKa = 3.61 GDD1270 pKa = 5.17 LEE1272 pKa = 4.06 QGYY1275 pKa = 9.65 CNRR1278 pKa = 11.84 KK1279 pKa = 6.7 RR1280 pKa = 11.84 TRR1282 pKa = 11.84 AAIAITAFLEE1292 pKa = 3.98 QTYY1295 pKa = 10.98 GDD1297 pKa = 3.84 TATILLGDD1305 pKa = 3.64 YY1306 pKa = 10.12 NAYY1309 pKa = 10.42 AKK1311 pKa = 10.09 EE1312 pKa = 4.2 DD1313 pKa = 4.73 PIQVLASAGYY1323 pKa = 8.55 ADD1325 pKa = 5.12 SVAEE1329 pKa = 4.1 MKK1331 pKa = 10.98 GNASYY1336 pKa = 10.83 SYY1338 pKa = 10.86 SYY1340 pKa = 11.21 DD1341 pKa = 3.28 GLAGTLDD1348 pKa = 3.68 YY1349 pKa = 11.09 MLVNQAAMDD1358 pKa = 3.57 MMVDD1362 pKa = 3.42 VNEE1365 pKa = 3.74 WHH1367 pKa = 7.04 INSDD1371 pKa = 3.31 EE1372 pKa = 4.24 PEE1374 pKa = 4.19 FYY1376 pKa = 10.39 DD1377 pKa = 4.28 YY1378 pKa = 11.73 NEE1380 pKa = 4.26 EE1381 pKa = 3.98 YY1382 pKa = 10.74 KK1383 pKa = 10.53 PASFLNEE1390 pKa = 3.74 LVYY1393 pKa = 10.4 RR1394 pKa = 11.84 ASDD1397 pKa = 3.64 HH1398 pKa = 7.11 DD1399 pKa = 4.37 PVIGTYY1405 pKa = 10.51 LLEE1408 pKa = 5.49 AEE1410 pKa = 4.7 EE1411 pKa = 4.58 EE1412 pKa = 4.45 PVTGDD1417 pKa = 2.66 WDD1419 pKa = 3.72 GDD1421 pKa = 3.76 GDD1423 pKa = 4.02 VDD1425 pKa = 5.45 RR1426 pKa = 11.84 DD1427 pKa = 3.8 DD1428 pKa = 3.51 VTALMRR1434 pKa = 11.84 AIFARR1439 pKa = 11.84 QEE1441 pKa = 3.33 IDD1443 pKa = 3.5 MSFDD1447 pKa = 3.72 LNNDD1451 pKa = 2.62 GRR1453 pKa = 11.84 VNARR1457 pKa = 11.84 DD1458 pKa = 3.17 IGAMRR1463 pKa = 11.84 RR1464 pKa = 11.84 MCTRR1468 pKa = 11.84 RR1469 pKa = 11.84 GCATEE1474 pKa = 4.55 DD1475 pKa = 3.25 ATPSGRR1481 pKa = 11.84 GRR1483 pKa = 11.84 TLASAVTTKK1492 pKa = 10.79 AAAISSLLNRR1502 pKa = 11.84 NRR1504 pKa = 3.36
Molecular weight: 159.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.554
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.605
Grimsley 3.465
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.897
Patrickios 1.392
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A1E7ZDC0|A0A1E7ZDC0_9ALTE Cobalamin biosynthesis protein CobQ OS=Alteromonas confluentis OX=1656094 GN=BFC18_07175 PE=4 SV=1
MM1 pKa = 7.1 EE2 pKa = 4.19 TRR4 pKa = 11.84 KK5 pKa = 10.16 FSVLTRR11 pKa = 11.84 GEE13 pKa = 4.02 RR14 pKa = 11.84 GVIAGVCSGLSEE26 pKa = 4.09 YY27 pKa = 10.9 FGFRR31 pKa = 11.84 KK32 pKa = 9.89 NCLRR36 pKa = 11.84 IAFVVFSFFFALPVMIYY53 pKa = 9.1 LTLWLILPKK62 pKa = 10.65 YY63 pKa = 6.89 PTSQAMARR71 pKa = 11.84 QLRR74 pKa = 11.84 RR75 pKa = 11.84 QAGRR79 pKa = 11.84 LSHH82 pKa = 7.36 DD83 pKa = 3.46 KK84 pKa = 11.1
Molecular weight: 9.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.867
IPC_protein 10.774
Toseland 10.774
ProMoST 10.628
Dawson 10.877
Bjellqvist 10.628
Wikipedia 11.125
Rodwell 11.038
Grimsley 10.935
Solomon 11.023
Lehninger 10.979
Nozaki 10.76
DTASelect 10.628
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.818
Patrickios 10.804
IPC_peptide 11.023
IPC2_peptide 9.838
IPC2.peptide.svr19 8.356
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4086
0
4086
1417638
37
2500
347.0
38.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.28 ± 0.038
0.941 ± 0.013
5.98 ± 0.04
6.225 ± 0.034
4.222 ± 0.027
7.033 ± 0.037
2.145 ± 0.018
5.849 ± 0.028
4.748 ± 0.035
9.93 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.64 ± 0.017
4.201 ± 0.024
4.092 ± 0.024
4.202 ± 0.028
4.743 ± 0.025
6.63 ± 0.029
5.625 ± 0.026
7.176 ± 0.026
1.279 ± 0.015
3.059 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here