Duganella sp. CF517

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Duganella; unclassified Duganella

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5879 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8I878|A0A1H8I878_9BURK Nitroreductase OS=Duganella sp. CF517 OX=1881038 GN=SAMN05428959_102733 PE=3 SV=1
MM1 pKa = 7.07NAVAEE6 pKa = 4.42SLVDD10 pKa = 4.24VIPSPIIFTDD20 pKa = 3.6SAAEE24 pKa = 4.01KK25 pKa = 9.59VAQLIEE31 pKa = 4.38EE32 pKa = 4.51EE33 pKa = 4.63GNPDD37 pKa = 2.85LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.94GFTFDD59 pKa = 4.69EE60 pKa = 4.93IVNEE64 pKa = 4.83DD65 pKa = 3.85DD66 pKa = 3.11TTMVKK71 pKa = 10.58NGVQLLIDD79 pKa = 3.77SMSYY83 pKa = 10.23QYY85 pKa = 11.55LVGAEE90 pKa = 4.01IDD92 pKa = 3.89YY93 pKa = 11.28KK94 pKa = 11.39DD95 pKa = 4.47DD96 pKa = 4.08LEE98 pKa = 4.56GAQFVIKK105 pKa = 10.68NPTATSTCGCGSSFSVV121 pKa = 3.54

Molecular weight: 13.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8FPY8|A0A1H8FPY8_9BURK Uncharacterized protein OS=Duganella sp. CF517 OX=1881038 GN=SAMN05428959_1011245 PE=4 SV=1
MM1 pKa = 7.59PPVPPLEE8 pKa = 3.82IHH10 pKa = 6.09IVLMVAAAGFLAGVQNALAGGGSFITFPALMLAGLNPLAANMTSTIALFPSQITSAYY67 pKa = 10.18AGRR70 pKa = 11.84QLVGDD75 pKa = 4.09VGPLTFRR82 pKa = 11.84QMLIISAIGGVFGAVLLLNTPASFFEE108 pKa = 4.88RR109 pKa = 11.84LVPWLVLFATSVFAWGSFRR128 pKa = 11.84KK129 pKa = 9.88KK130 pKa = 9.63PLHH133 pKa = 6.35AAGGMPRR140 pKa = 11.84WVLALAQGCIAIYY153 pKa = 10.22GGYY156 pKa = 9.69FGGGIGFLMLAALTVAGQQVRR177 pKa = 11.84MAAATKK183 pKa = 10.41NVLAMAMNAVAALIFATSGLISWPAALALCVGGIAGGLCGGWLIHH228 pKa = 6.6RR229 pKa = 11.84LPEE232 pKa = 3.68KK233 pKa = 10.42VMRR236 pKa = 11.84GFVVLVGAALTVWMFVRR253 pKa = 4.91

Molecular weight: 26.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5879

0

5879

2143361

29

5493

364.6

39.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.873 ± 0.058

0.799 ± 0.009

5.556 ± 0.028

4.919 ± 0.033

3.515 ± 0.02

8.478 ± 0.071

2.115 ± 0.015

4.643 ± 0.024

3.675 ± 0.031

10.325 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.015

3.15 ± 0.023

4.994 ± 0.025

3.871 ± 0.025

6.372 ± 0.032

5.556 ± 0.027

5.381 ± 0.035

7.305 ± 0.025

1.368 ± 0.015

2.594 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski