Marinobacter phage PS6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GMX0|A0A2D1GMX0_9CAUD Uncharacterized protein OS=Marinobacter phage PS6 OX=2041342 PE=4 SV=1
MM1 pKa = 7.75ASILGKK7 pKa = 10.34LAIQEE12 pKa = 4.24GDD14 pKa = 3.63SYY16 pKa = 11.13TAAAGSNRR24 pKa = 11.84AVVVAGTGEE33 pKa = 4.22SAFSSRR39 pKa = 11.84EE40 pKa = 3.51IVGITFGGVAMTRR53 pKa = 11.84AAYY56 pKa = 9.13TGDD59 pKa = 3.4TEE61 pKa = 4.34TAKK64 pKa = 11.02GGVYY68 pKa = 10.39YY69 pKa = 9.99ILEE72 pKa = 4.19SQIPAGSSVIAVDD85 pKa = 3.06WDD87 pKa = 4.15GQVHH91 pKa = 6.65DD92 pKa = 5.02AFTGAVYY99 pKa = 9.74TLGGIDD105 pKa = 3.85QATPVNNFNSAVGVSLFDD123 pKa = 4.62LDD125 pKa = 4.19IMFNGVDD132 pKa = 3.6GGVLIVGAGSNSPADD147 pKa = 3.83TLNQSSTISATTTTVTVDD165 pKa = 3.36HH166 pKa = 6.7AQTTAGHH173 pKa = 6.31EE174 pKa = 4.37GSAISGVLSAAGSEE188 pKa = 4.43TLGLRR193 pKa = 11.84YY194 pKa = 9.81SSEE197 pKa = 4.03TDD199 pKa = 3.25PVGALAVFNPATSGPSVSAADD220 pKa = 5.14DD221 pKa = 3.5ITSEE225 pKa = 4.05GDD227 pKa = 3.45TVEE230 pKa = 4.67FTLADD235 pKa = 3.46NSAAPVSATLNGTDD249 pKa = 3.31VGTLTLVSGSTYY261 pKa = 10.69SYY263 pKa = 8.06TAPLIADD270 pKa = 4.93DD271 pKa = 4.29DD272 pKa = 4.27TADD275 pKa = 3.85LVVSVDD281 pKa = 3.16STTASTVISYY291 pKa = 10.96ANSYY295 pKa = 9.75PYY297 pKa = 10.77EE298 pKa = 4.28LVTHH302 pKa = 6.53GEE304 pKa = 3.87PDD306 pKa = 3.3ANSAFFDD313 pKa = 3.72TAFATDD319 pKa = 5.15GPVEE323 pKa = 4.21WGVVTDD329 pKa = 4.29FDD331 pKa = 4.22SGVVVVDD338 pKa = 3.89WAAMDD343 pKa = 3.88AAEE346 pKa = 6.02DD347 pKa = 3.98EE348 pKa = 4.82LNDD351 pKa = 3.49IALHH355 pKa = 4.6STEE358 pKa = 4.18VAAGDD363 pKa = 3.56STATLKK369 pKa = 11.1YY370 pKa = 9.44FVPEE374 pKa = 4.12SGATGTFQATATVDD388 pKa = 3.81GTDD391 pKa = 3.3EE392 pKa = 4.01TAPTISSASVPTAGNNIAVQMSEE415 pKa = 3.9SMQVGAGGSGGWTISLGDD433 pKa = 3.65ATVTSAAVDD442 pKa = 3.99GSDD445 pKa = 3.37DD446 pKa = 3.65TTVNLTPSRR455 pKa = 11.84TLTDD459 pKa = 3.41EE460 pKa = 4.01DD461 pKa = 4.96TFTIGYY467 pKa = 5.39TQPGNGFQDD476 pKa = 3.77QADD479 pKa = 4.34TPNDD483 pKa = 3.51LATLSGQVVTNNSTQQPPDD502 pKa = 3.08VTGPVIVSVSVPTTGTYY519 pKa = 10.89APGDD523 pKa = 3.61ALNFTVNFDD532 pKa = 3.66EE533 pKa = 4.25NTFRR537 pKa = 11.84TGVPALNLDD546 pKa = 3.67VGGSARR552 pKa = 11.84QANYY556 pKa = 10.53VSGTDD561 pKa = 3.15TTALVFRR568 pKa = 11.84YY569 pKa = 7.21TVQEE573 pKa = 3.98GDD575 pKa = 3.72EE576 pKa = 4.31DD577 pKa = 4.29TDD579 pKa = 4.89GITVSSLTLDD589 pKa = 3.43GGTLKK594 pKa = 10.99DD595 pKa = 3.59SAGNDD600 pKa = 3.04ATLTLNGVGDD610 pKa = 3.78TSGILIDD617 pKa = 4.5GVSPTISIGAINTTDD632 pKa = 3.44TTPTASGSAGDD643 pKa = 4.27SDD645 pKa = 5.75SLTLDD650 pKa = 3.42LDD652 pKa = 4.39GVDD655 pKa = 3.8VTHH658 pKa = 6.82TSSYY662 pKa = 11.09NITPSGGSWSQQFPEE677 pKa = 4.57LAIGTYY683 pKa = 9.68TMTLTGVDD691 pKa = 3.25TAGNEE696 pKa = 4.36TVEE699 pKa = 4.31TATLQIVEE707 pKa = 4.77PSTSPSSKK715 pKa = 10.5LLRR718 pKa = 11.84QLLRR722 pKa = 11.84GSLRR726 pKa = 11.84SSLKK730 pKa = 10.73LSS732 pKa = 3.51

Molecular weight: 74.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GMP0|A0A2D1GMP0_9CAUD Uncharacterized protein OS=Marinobacter phage PS6 OX=2041342 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84HH3 pKa = 5.47MGYY6 pKa = 9.98EE7 pKa = 3.83PRR9 pKa = 11.84EE10 pKa = 3.59IWEE13 pKa = 4.67DD14 pKa = 3.65LNEE17 pKa = 4.07QPHH20 pKa = 5.97IVRR23 pKa = 11.84KK24 pKa = 10.04IISDD28 pKa = 3.37HH29 pKa = 5.24VNRR32 pKa = 11.84YY33 pKa = 8.65SPEE36 pKa = 3.67RR37 pKa = 11.84VEE39 pKa = 6.15AIRR42 pKa = 11.84QTRR45 pKa = 11.84KK46 pKa = 9.32RR47 pKa = 11.84QRR49 pKa = 11.84QNKK52 pKa = 6.7ATRR55 pKa = 11.84KK56 pKa = 9.66RR57 pKa = 11.84LAEE60 pKa = 3.95QQQ62 pKa = 3.2

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

18163

41

1500

227.0

25.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.526 ± 0.474

0.864 ± 0.117

7.086 ± 0.276

7.185 ± 0.411

4.019 ± 0.185

8.016 ± 0.344

1.767 ± 0.163

5.049 ± 0.171

5.296 ± 0.367

7.741 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.209

4.454 ± 0.173

4.184 ± 0.264

4.008 ± 0.33

5.963 ± 0.386

6.172 ± 0.293

6.524 ± 0.432

6.794 ± 0.301

1.63 ± 0.156

3.254 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski