Amino acid dipepetide frequency for Marinobacter phage PS6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.552AlaAla: 6.552 ± 1.151
0.661AlaCys: 0.661 ± 0.198
5.066AlaAsp: 5.066 ± 0.69
4.405AlaGlu: 4.405 ± 0.629
3.524AlaPhe: 3.524 ± 0.38
7.213AlaGly: 7.213 ± 0.697
0.991AlaHis: 0.991 ± 0.251
4.9AlaIle: 4.9 ± 0.68
3.359AlaLys: 3.359 ± 0.54
6.827AlaLeu: 6.827 ± 0.647
1.817AlaMet: 1.817 ± 0.316
2.918AlaAsn: 2.918 ± 0.419
2.368AlaPro: 2.368 ± 0.353
2.753AlaGln: 2.753 ± 0.844
3.634AlaArg: 3.634 ± 0.489
4.515AlaSer: 4.515 ± 0.639
5.01AlaThr: 5.01 ± 0.631
5.396AlaVal: 5.396 ± 0.473
0.881AlaTrp: 0.881 ± 0.255
2.478AlaTyr: 2.478 ± 0.338
0.0AlaXaa: 0.0 ± 0.0
Cys
0.551CysAla: 0.551 ± 0.185
0.165CysCys: 0.165 ± 0.089
0.771CysAsp: 0.771 ± 0.184
0.826CysGlu: 0.826 ± 0.243
0.771CysPhe: 0.771 ± 0.212
0.606CysGly: 0.606 ± 0.228
0.165CysHis: 0.165 ± 0.089
0.385CysIle: 0.385 ± 0.148
0.33CysLys: 0.33 ± 0.135
0.275CysLeu: 0.275 ± 0.119
0.165CysMet: 0.165 ± 0.095
0.275CysAsn: 0.275 ± 0.108
0.496CysPro: 0.496 ± 0.194
0.22CysGln: 0.22 ± 0.111
0.606CysArg: 0.606 ± 0.179
0.44CysSer: 0.44 ± 0.186
0.496CysThr: 0.496 ± 0.222
0.385CysVal: 0.385 ± 0.149
0.385CysTrp: 0.385 ± 0.123
0.33CysTyr: 0.33 ± 0.137
0.0CysXaa: 0.0 ± 0.0
Asp
5.066AspAla: 5.066 ± 0.55
0.771AspCys: 0.771 ± 0.221
5.836AspAsp: 5.836 ± 1.081
5.781AspGlu: 5.781 ± 0.585
2.863AspPhe: 2.863 ± 0.382
6.332AspGly: 6.332 ± 0.708
1.156AspHis: 1.156 ± 0.227
3.359AspIle: 3.359 ± 0.422
2.313AspLys: 2.313 ± 0.323
6.387AspLeu: 6.387 ± 0.678
2.257AspMet: 2.257 ± 0.415
2.918AspAsn: 2.918 ± 0.341
3.028AspPro: 3.028 ± 0.403
3.193AspGln: 3.193 ± 0.411
4.074AspArg: 4.074 ± 0.703
4.019AspSer: 4.019 ± 0.42
4.405AspThr: 4.405 ± 0.658
3.359AspVal: 3.359 ± 0.507
1.377AspTrp: 1.377 ± 0.297
2.368AspTyr: 2.368 ± 0.482
0.0AspXaa: 0.0 ± 0.0
Glu
4.955GluAla: 4.955 ± 0.648
0.606GluCys: 0.606 ± 0.187
4.35GluAsp: 4.35 ± 0.646
4.68GluGlu: 4.68 ± 0.799
3.304GluPhe: 3.304 ± 0.462
5.121GluGly: 5.121 ± 0.441
1.707GluHis: 1.707 ± 0.348
4.185GluIle: 4.185 ± 0.472
3.909GluLys: 3.909 ± 0.661
6.387GluLeu: 6.387 ± 0.751
1.377GluMet: 1.377 ± 0.213
3.193GluAsn: 3.193 ± 0.382
2.478GluPro: 2.478 ± 0.37
3.083GluGln: 3.083 ± 0.605
5.286GluArg: 5.286 ± 0.822
4.35GluSer: 4.35 ± 0.509
5.176GluThr: 5.176 ± 0.569
4.845GluVal: 4.845 ± 0.532
1.046GluTrp: 1.046 ± 0.208
1.817GluTyr: 1.817 ± 0.322
0.0GluXaa: 0.0 ± 0.0
Phe
2.753PheAla: 2.753 ± 0.414
0.33PheCys: 0.33 ± 0.112
4.019PheAsp: 4.019 ± 0.37
2.918PheGlu: 2.918 ± 0.463
0.936PhePhe: 0.936 ± 0.227
3.138PheGly: 3.138 ± 0.407
0.33PheHis: 0.33 ± 0.123
1.982PheIle: 1.982 ± 0.358
2.478PheLys: 2.478 ± 0.412
2.423PheLeu: 2.423 ± 0.366
1.211PheMet: 1.211 ± 0.257
2.533PheAsn: 2.533 ± 0.379
1.982PhePro: 1.982 ± 0.413
1.156PheGln: 1.156 ± 0.24
2.478PheArg: 2.478 ± 0.287
2.423PheSer: 2.423 ± 0.358
2.973PheThr: 2.973 ± 0.528
2.368PheVal: 2.368 ± 0.321
0.496PheTrp: 0.496 ± 0.165
1.266PheTyr: 1.266 ± 0.284
0.0PheXaa: 0.0 ± 0.0
Gly
5.286GlyAla: 5.286 ± 0.506
0.826GlyCys: 0.826 ± 0.263
5.506GlyAsp: 5.506 ± 0.621
6.607GlyGlu: 6.607 ± 0.509
2.808GlyPhe: 2.808 ± 0.528
5.671GlyGly: 5.671 ± 0.739
1.432GlyHis: 1.432 ± 0.297
4.074GlyIle: 4.074 ± 0.378
5.066GlyLys: 5.066 ± 0.557
6.112GlyLeu: 6.112 ± 0.576
2.147GlyMet: 2.147 ± 0.305
3.414GlyAsn: 3.414 ± 0.459
1.982GlyPro: 1.982 ± 0.365
3.524GlyGln: 3.524 ± 0.429
4.955GlyArg: 4.955 ± 0.626
4.845GlySer: 4.845 ± 0.698
4.955GlyThr: 4.955 ± 0.652
5.836GlyVal: 5.836 ± 0.726
1.432GlyTrp: 1.432 ± 0.28
3.634GlyTyr: 3.634 ± 0.592
0.0GlyXaa: 0.0 ± 0.0
His
0.716HisAla: 0.716 ± 0.199
0.44HisCys: 0.44 ± 0.167
0.881HisAsp: 0.881 ± 0.228
1.046HisGlu: 1.046 ± 0.274
1.211HisPhe: 1.211 ± 0.341
1.432HisGly: 1.432 ± 0.292
0.275HisHis: 0.275 ± 0.127
1.542HisIle: 1.542 ± 0.278
0.881HisLys: 0.881 ± 0.25
1.597HisLeu: 1.597 ± 0.335
0.33HisMet: 0.33 ± 0.117
0.771HisAsn: 0.771 ± 0.207
0.881HisPro: 0.881 ± 0.193
0.33HisGln: 0.33 ± 0.109
1.211HisArg: 1.211 ± 0.307
1.432HisSer: 1.432 ± 0.251
0.881HisThr: 0.881 ± 0.242
0.991HisVal: 0.991 ± 0.243
0.165HisTrp: 0.165 ± 0.091
0.661HisTyr: 0.661 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
3.909IleAla: 3.909 ± 0.577
0.385IleCys: 0.385 ± 0.112
4.13IleAsp: 4.13 ± 0.358
4.074IleGlu: 4.074 ± 0.555
1.101IlePhe: 1.101 ± 0.191
3.689IleGly: 3.689 ± 0.474
1.156IleHis: 1.156 ± 0.254
1.872IleIle: 1.872 ± 0.336
2.257IleLys: 2.257 ± 0.366
3.359IleLeu: 3.359 ± 0.411
1.101IleMet: 1.101 ± 0.316
3.138IleAsn: 3.138 ± 0.434
1.982IlePro: 1.982 ± 0.4
1.872IleGln: 1.872 ± 0.244
3.854IleArg: 3.854 ± 0.5
2.973IleSer: 2.973 ± 0.47
3.799IleThr: 3.799 ± 0.449
3.524IleVal: 3.524 ± 0.479
0.771IleTrp: 0.771 ± 0.216
1.542IleTyr: 1.542 ± 0.315
0.0IleXaa: 0.0 ± 0.0
Lys
5.341LysAla: 5.341 ± 0.832
0.33LysCys: 0.33 ± 0.148
3.249LysAsp: 3.249 ± 0.357
3.689LysGlu: 3.689 ± 0.557
1.817LysPhe: 1.817 ± 0.267
4.35LysGly: 4.35 ± 0.46
0.771LysHis: 0.771 ± 0.2
2.147LysIle: 2.147 ± 0.31
3.689LysLys: 3.689 ± 0.571
3.524LysLeu: 3.524 ± 0.513
1.982LysMet: 1.982 ± 0.362
2.313LysAsn: 2.313 ± 0.413
2.808LysPro: 2.808 ± 0.378
2.257LysGln: 2.257 ± 0.399
3.083LysArg: 3.083 ± 0.396
2.643LysSer: 2.643 ± 0.46
3.524LysThr: 3.524 ± 0.552
3.193LysVal: 3.193 ± 0.452
0.716LysTrp: 0.716 ± 0.238
1.542LysTyr: 1.542 ± 0.341
0.0LysXaa: 0.0 ± 0.0
Leu
6.552LeuAla: 6.552 ± 0.672
0.551LeuCys: 0.551 ± 0.169
6.277LeuAsp: 6.277 ± 0.536
5.726LeuGlu: 5.726 ± 0.578
2.918LeuPhe: 2.918 ± 0.515
5.396LeuGly: 5.396 ± 0.605
1.266LeuHis: 1.266 ± 0.259
3.083LeuIle: 3.083 ± 0.425
4.955LeuLys: 4.955 ± 0.669
5.286LeuLeu: 5.286 ± 0.509
2.092LeuMet: 2.092 ± 0.398
4.24LeuAsn: 4.24 ± 0.462
3.304LeuPro: 3.304 ± 0.439
2.808LeuGln: 2.808 ± 0.499
4.735LeuArg: 4.735 ± 0.479
4.955LeuSer: 4.955 ± 0.459
5.176LeuThr: 5.176 ± 0.616
5.396LeuVal: 5.396 ± 0.472
0.606LeuTrp: 0.606 ± 0.182
2.092LeuTyr: 2.092 ± 0.414
0.0LeuXaa: 0.0 ± 0.0
Met
1.927MetAla: 1.927 ± 0.333
0.055MetCys: 0.055 ± 0.051
1.927MetAsp: 1.927 ± 0.392
1.432MetGlu: 1.432 ± 0.34
0.881MetPhe: 0.881 ± 0.167
1.927MetGly: 1.927 ± 0.353
0.44MetHis: 0.44 ± 0.244
1.487MetIle: 1.487 ± 0.305
1.982MetLys: 1.982 ± 0.408
1.487MetLeu: 1.487 ± 0.324
0.881MetMet: 0.881 ± 0.226
0.936MetAsn: 0.936 ± 0.213
1.046MetPro: 1.046 ± 0.222
0.771MetGln: 0.771 ± 0.204
1.377MetArg: 1.377 ± 0.272
2.092MetSer: 2.092 ± 0.308
1.872MetThr: 1.872 ± 0.32
1.542MetVal: 1.542 ± 0.271
0.165MetTrp: 0.165 ± 0.098
0.44MetTyr: 0.44 ± 0.184
0.0MetXaa: 0.0 ± 0.0
Asn
3.854AsnAla: 3.854 ± 0.613
0.275AsnCys: 0.275 ± 0.113
2.973AsnAsp: 2.973 ± 0.376
2.808AsnGlu: 2.808 ± 0.289
1.652AsnPhe: 1.652 ± 0.289
4.515AsnGly: 4.515 ± 0.505
0.881AsnHis: 0.881 ± 0.188
1.982AsnIle: 1.982 ± 0.333
1.817AsnLys: 1.817 ± 0.325
2.808AsnLeu: 2.808 ± 0.375
0.496AsnMet: 0.496 ± 0.141
1.872AsnAsn: 1.872 ± 0.318
2.533AsnPro: 2.533 ± 0.488
1.927AsnGln: 1.927 ± 0.3
3.083AsnArg: 3.083 ± 0.405
2.753AsnSer: 2.753 ± 0.519
2.973AsnThr: 2.973 ± 0.355
1.817AsnVal: 1.817 ± 0.302
1.597AsnTrp: 1.597 ± 0.312
1.927AsnTyr: 1.927 ± 0.365
0.0AsnXaa: 0.0 ± 0.0
Pro
2.918ProAla: 2.918 ± 0.377
0.22ProCys: 0.22 ± 0.122
3.579ProAsp: 3.579 ± 0.379
3.359ProGlu: 3.359 ± 0.491
1.762ProPhe: 1.762 ± 0.335
4.074ProGly: 4.074 ± 0.631
0.881ProHis: 0.881 ± 0.267
1.597ProIle: 1.597 ± 0.319
1.982ProLys: 1.982 ± 0.403
2.202ProLeu: 2.202 ± 0.333
1.266ProMet: 1.266 ± 0.308
1.266ProAsn: 1.266 ± 0.295
1.982ProPro: 1.982 ± 0.413
0.991ProGln: 0.991 ± 0.2
1.872ProArg: 1.872 ± 0.318
3.414ProSer: 3.414 ± 0.415
2.423ProThr: 2.423 ± 0.413
3.524ProVal: 3.524 ± 0.576
0.716ProTrp: 0.716 ± 0.188
1.817ProTyr: 1.817 ± 0.286
0.0ProXaa: 0.0 ± 0.0
Gln
3.414GlnAla: 3.414 ± 0.647
0.165GlnCys: 0.165 ± 0.098
2.202GlnAsp: 2.202 ± 0.347
2.808GlnGlu: 2.808 ± 0.614
1.321GlnPhe: 1.321 ± 0.279
3.028GlnGly: 3.028 ± 0.369
0.44GlnHis: 0.44 ± 0.164
2.092GlnIle: 2.092 ± 0.312
2.533GlnLys: 2.533 ± 0.497
3.579GlnLeu: 3.579 ± 0.472
0.991GlnMet: 0.991 ± 0.244
2.147GlnAsn: 2.147 ± 0.339
1.321GlnPro: 1.321 ± 0.301
1.707GlnGln: 1.707 ± 0.416
2.202GlnArg: 2.202 ± 0.52
2.753GlnSer: 2.753 ± 0.61
2.202GlnThr: 2.202 ± 0.276
3.028GlnVal: 3.028 ± 0.47
0.881GlnTrp: 0.881 ± 0.243
1.266GlnTyr: 1.266 ± 0.254
0.0GlnXaa: 0.0 ± 0.0
Arg
3.469ArgAla: 3.469 ± 0.426
0.716ArgCys: 0.716 ± 0.224
3.634ArgAsp: 3.634 ± 0.514
4.955ArgGlu: 4.955 ± 0.845
3.193ArgPhe: 3.193 ± 0.456
3.414ArgGly: 3.414 ± 0.443
1.432ArgHis: 1.432 ± 0.303
2.588ArgIle: 2.588 ± 0.464
3.909ArgLys: 3.909 ± 0.56
6.002ArgLeu: 6.002 ± 0.602
1.652ArgMet: 1.652 ± 0.316
1.982ArgAsn: 1.982 ± 0.351
1.982ArgPro: 1.982 ± 0.406
2.918ArgGln: 2.918 ± 0.743
4.845ArgArg: 4.845 ± 0.885
2.973ArgSer: 2.973 ± 0.412
3.138ArgThr: 3.138 ± 0.371
4.074ArgVal: 4.074 ± 0.485
0.991ArgTrp: 0.991 ± 0.307
1.762ArgTyr: 1.762 ± 0.317
0.0ArgXaa: 0.0 ± 0.0
Ser
5.066SerAla: 5.066 ± 0.703
0.496SerCys: 0.496 ± 0.166
4.405SerAsp: 4.405 ± 0.55
4.625SerGlu: 4.625 ± 0.479
2.863SerPhe: 2.863 ± 0.425
5.946SerGly: 5.946 ± 0.676
0.551SerHis: 0.551 ± 0.201
2.863SerIle: 2.863 ± 0.413
2.533SerLys: 2.533 ± 0.334
5.176SerLeu: 5.176 ± 0.488
1.321SerMet: 1.321 ± 0.301
2.808SerAsn: 2.808 ± 0.414
3.304SerPro: 3.304 ± 0.479
2.202SerGln: 2.202 ± 0.331
2.313SerArg: 2.313 ± 0.415
3.744SerSer: 3.744 ± 0.655
3.744SerThr: 3.744 ± 0.495
4.35SerVal: 4.35 ± 0.562
0.881SerTrp: 0.881 ± 0.163
2.533SerTyr: 2.533 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
4.625ThrAla: 4.625 ± 0.745
0.716ThrCys: 0.716 ± 0.187
4.35ThrAsp: 4.35 ± 0.643
3.744ThrGlu: 3.744 ± 0.384
2.257ThrPhe: 2.257 ± 0.304
6.057ThrGly: 6.057 ± 0.68
1.156ThrHis: 1.156 ± 0.279
3.249ThrIle: 3.249 ± 0.327
2.863ThrLys: 2.863 ± 0.401
6.057ThrLeu: 6.057 ± 0.822
1.156ThrMet: 1.156 ± 0.24
2.533ThrAsn: 2.533 ± 0.311
2.973ThrPro: 2.973 ± 0.479
3.193ThrGln: 3.193 ± 0.675
3.138ThrArg: 3.138 ± 0.484
3.524ThrSer: 3.524 ± 0.54
4.35ThrThr: 4.35 ± 0.552
6.277ThrVal: 6.277 ± 0.736
1.266ThrTrp: 1.266 ± 0.459
1.762ThrTyr: 1.762 ± 0.384
0.0ThrXaa: 0.0 ± 0.0
Val
4.735ValAla: 4.735 ± 0.526
0.33ValCys: 0.33 ± 0.159
4.625ValAsp: 4.625 ± 0.581
5.671ValGlu: 5.671 ± 0.457
2.918ValPhe: 2.918 ± 0.359
5.121ValGly: 5.121 ± 0.487
1.211ValHis: 1.211 ± 0.285
3.964ValIle: 3.964 ± 0.453
3.414ValLys: 3.414 ± 0.498
4.625ValLeu: 4.625 ± 0.592
1.211ValMet: 1.211 ± 0.263
3.359ValAsn: 3.359 ± 0.525
3.193ValPro: 3.193 ± 0.396
2.478ValGln: 2.478 ± 0.302
4.019ValArg: 4.019 ± 0.387
4.955ValSer: 4.955 ± 0.605
4.625ValThr: 4.625 ± 0.609
4.57ValVal: 4.57 ± 0.604
1.211ValTrp: 1.211 ± 0.212
1.707ValTyr: 1.707 ± 0.385
0.0ValXaa: 0.0 ± 0.0
Trp
1.377TrpAla: 1.377 ± 0.263
0.055TrpCys: 0.055 ± 0.063
1.046TrpAsp: 1.046 ± 0.222
0.661TrpGlu: 0.661 ± 0.167
0.991TrpPhe: 0.991 ± 0.273
1.101TrpGly: 1.101 ± 0.339
0.44TrpHis: 0.44 ± 0.141
1.156TrpIle: 1.156 ± 0.281
1.156TrpLys: 1.156 ± 0.209
1.266TrpLeu: 1.266 ± 0.305
0.22TrpMet: 0.22 ± 0.088
0.496TrpAsn: 0.496 ± 0.194
0.771TrpPro: 0.771 ± 0.18
0.661TrpGln: 0.661 ± 0.168
0.716TrpArg: 0.716 ± 0.177
1.211TrpSer: 1.211 ± 0.287
0.771TrpThr: 0.771 ± 0.215
1.542TrpVal: 1.542 ± 0.271
0.11TrpTrp: 0.11 ± 0.082
0.551TrpTyr: 0.551 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.202TyrAla: 2.202 ± 0.397
0.551TyrCys: 0.551 ± 0.183
2.037TyrAsp: 2.037 ± 0.355
2.037TyrGlu: 2.037 ± 0.392
1.101TyrPhe: 1.101 ± 0.253
2.037TyrGly: 2.037 ± 0.428
0.991TyrHis: 0.991 ± 0.258
1.982TyrIle: 1.982 ± 0.327
1.542TyrLys: 1.542 ± 0.297
2.037TyrLeu: 2.037 ± 0.453
0.936TyrMet: 0.936 ± 0.236
1.432TyrAsn: 1.432 ± 0.278
1.432TyrPro: 1.432 ± 0.33
2.037TyrGln: 2.037 ± 0.328
2.147TyrArg: 2.147 ± 0.349
1.707TyrSer: 1.707 ± 0.336
2.753TyrThr: 2.753 ± 0.398
1.927TyrVal: 1.927 ± 0.276
0.606TyrTrp: 0.606 ± 0.191
1.046TyrTyr: 1.046 ± 0.259
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (18163 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski