Streptomyces sp. MZ04

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8167 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R9EIA3|A0A4R9EIA3_9ACTN Ectoine/hydroxyectoine ABC transporter permease subunit EhuC OS=Streptomyces sp. MZ04 OX=2559236 GN=ehuC PE=3 SV=1
MM1 pKa = 7.69VGLTWKK7 pKa = 9.62QAKK10 pKa = 9.62KK11 pKa = 10.79KK12 pKa = 9.32LGKK15 pKa = 7.04WAKK18 pKa = 10.46YY19 pKa = 10.42ADD21 pKa = 4.12TYY23 pKa = 10.81KK24 pKa = 10.67AWSDD28 pKa = 3.37QKK30 pKa = 11.2DD31 pKa = 3.81VPEE34 pKa = 4.42VGDD37 pKa = 4.83DD38 pKa = 4.2IVICFQDD45 pKa = 3.34KK46 pKa = 10.8PEE48 pKa = 3.91YY49 pKa = 10.37LNPGEE54 pKa = 4.89EE55 pKa = 4.88DD56 pKa = 3.44PDD58 pKa = 4.24LNLGLAAVSAGSACPKK74 pKa = 10.61KK75 pKa = 10.73NGDD78 pKa = 3.75YY79 pKa = 10.65LDD81 pKa = 4.95PGNDD85 pKa = 3.83PDD87 pKa = 4.37SPSYY91 pKa = 11.28DD92 pKa = 3.43EE93 pKa = 5.9DD94 pKa = 4.22NNGVPDD100 pKa = 3.87SQEE103 pKa = 4.9DD104 pKa = 3.65DD105 pKa = 3.93DD106 pKa = 5.97SYY108 pKa = 11.86GSSGSSSTGGGSSTSGGGGDD128 pKa = 4.28NPADD132 pKa = 3.85GPDD135 pKa = 4.05DD136 pKa = 3.98YY137 pKa = 11.12EE138 pKa = 5.13HH139 pKa = 7.08SGGGGCPPGGCNNPCPPGGCTSS161 pKa = 3.28

Molecular weight: 16.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R9EV93|A0A4R9EV93_9ACTN Preprotein translocase subunit YajC OS=Streptomyces sp. MZ04 OX=2559236 GN=yajC PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8167

0

8167

2593571

19

4088

317.6

34.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.394 ± 0.043

0.813 ± 0.008

6.059 ± 0.024

5.803 ± 0.026

2.758 ± 0.017

9.434 ± 0.025

2.269 ± 0.014

3.372 ± 0.019

2.571 ± 0.024

10.199 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.801 ± 0.012

1.809 ± 0.012

5.934 ± 0.023

2.833 ± 0.014

7.784 ± 0.032

5.23 ± 0.018

6.034 ± 0.021

8.248 ± 0.025

1.533 ± 0.012

2.123 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski