Amino acid dipepetide frequency for Streptomyces sp. MZ04

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.902AlaAla: 20.902 ± 0.13	1.072AlaCys: 1.072 ± 0.02	8.481AlaAsp: 8.481 ± 0.065	8.825AlaGlu: 8.825 ± 0.087	3.526AlaPhe: 3.526 ± 0.036	12.413AlaGly: 12.413 ± 0.078	2.776AlaHis: 2.776 ± 0.037	3.769AlaIle: 3.769 ± 0.048	3.343AlaLys: 3.343 ± 0.051	14.131AlaLeu: 14.131 ± 0.099	2.591AlaMet: 2.591 ± 0.033	1.912AlaAsn: 1.912 ± 0.032	6.913AlaPro: 6.913 ± 0.065	3.819AlaGln: 3.819 ± 0.042	9.827AlaArg: 9.827 ± 0.075	6.125AlaSer: 6.125 ± 0.055	6.868AlaThr: 6.868 ± 0.057	11.847AlaVal: 11.847 ± 0.081	1.883AlaTrp: 1.883 ± 0.027	2.922AlaTyr: 2.922 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.097CysAla: 1.097 ± 0.022	0.098CysCys: 0.098 ± 0.007	0.472CysAsp: 0.472 ± 0.013	0.41CysGlu: 0.41 ± 0.014	0.228CysPhe: 0.228 ± 0.009	1.007CysGly: 1.007 ± 0.02	0.199CysHis: 0.199 ± 0.008	0.185CysIle: 0.185 ± 0.008	0.136CysLys: 0.136 ± 0.008	0.761CysLeu: 0.761 ± 0.017	0.133CysMet: 0.133 ± 0.007	0.14CysAsn: 0.14 ± 0.008	0.49CysPro: 0.49 ± 0.017	0.182CysGln: 0.182 ± 0.009	0.613CysArg: 0.613 ± 0.016	0.455CysSer: 0.455 ± 0.013	0.515CysThr: 0.515 ± 0.013	0.721CysVal: 0.721 ± 0.017	0.132CysTrp: 0.132 ± 0.006	0.155CysTyr: 0.155 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.937AspAla: 7.937 ± 0.062	0.456AspCys: 0.456 ± 0.014	3.779AspAsp: 3.779 ± 0.044	4.087AspGlu: 4.087 ± 0.047	1.685AspPhe: 1.685 ± 0.027	6.457AspGly: 6.457 ± 0.061	1.392AspHis: 1.392 ± 0.026	1.995AspIle: 1.995 ± 0.029	1.511AspLys: 1.511 ± 0.029	6.081AspLeu: 6.081 ± 0.053	0.902AspMet: 0.902 ± 0.018	0.957AspAsn: 0.957 ± 0.022	4.373AspPro: 4.373 ± 0.051	1.59AspGln: 1.59 ± 0.027	4.669AspArg: 4.669 ± 0.049	2.657AspSer: 2.657 ± 0.034	3.132AspThr: 3.132 ± 0.04	4.74AspVal: 4.74 ± 0.043	1.039AspTrp: 1.039 ± 0.02	1.148AspTyr: 1.148 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.413GluAla: 7.413 ± 0.083	0.39GluCys: 0.39 ± 0.012	2.726GluAsp: 2.726 ± 0.034	3.492GluGlu: 3.492 ± 0.048	1.528GluPhe: 1.528 ± 0.028	4.472GluGly: 4.472 ± 0.044	1.525GluHis: 1.525 ± 0.026	2.355GluIle: 2.355 ± 0.033	1.713GluLys: 1.713 ± 0.031	7.07GluLeu: 7.07 ± 0.066	0.914GluMet: 0.914 ± 0.022	1.071GluAsn: 1.071 ± 0.021	3.472GluPro: 3.472 ± 0.043	2.319GluGln: 2.319 ± 0.038	5.54GluArg: 5.54 ± 0.057	2.824GluSer: 2.824 ± 0.039	2.902GluThr: 2.902 ± 0.034	4.343GluVal: 4.343 ± 0.046	0.809GluTrp: 0.809 ± 0.017	1.157GluTyr: 1.157 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	3.751PheAla: 3.751 ± 0.042	0.276PheCys: 0.276 ± 0.011	1.967PheAsp: 1.967 ± 0.036	1.449PheGlu: 1.449 ± 0.024	0.872PhePhe: 0.872 ± 0.019	3.068PheGly: 3.068 ± 0.035	0.583PheHis: 0.583 ± 0.015	0.819PheIle: 0.819 ± 0.017	0.635PheLys: 0.635 ± 0.017	2.575PheLeu: 2.575 ± 0.032	0.44PheMet: 0.44 ± 0.014	0.603PheAsn: 0.603 ± 0.015	1.38PhePro: 1.38 ± 0.024	0.674PheGln: 0.674 ± 0.015	1.826PheArg: 1.826 ± 0.031	1.489PheSer: 1.489 ± 0.026	1.978PheThr: 1.978 ± 0.029	2.203PheVal: 2.203 ± 0.031	0.43PheTrp: 0.43 ± 0.012	0.564PheTyr: 0.564 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	11.041GlyAla: 11.041 ± 0.069	0.846GlyCys: 0.846 ± 0.017	5.342GlyAsp: 5.342 ± 0.045	5.29GlyGlu: 5.29 ± 0.048	2.893GlyPhe: 2.893 ± 0.032	8.959GlyGly: 8.959 ± 0.087	2.261GlyHis: 2.261 ± 0.033	3.635GlyIle: 3.635 ± 0.041	2.949GlyLys: 2.949 ± 0.044	9.321GlyLeu: 9.321 ± 0.076	2.029GlyMet: 2.029 ± 0.03	1.815GlyAsn: 1.815 ± 0.036	4.859GlyPro: 4.859 ± 0.046	2.761GlyGln: 2.761 ± 0.037	7.26GlyArg: 7.26 ± 0.066	5.567GlySer: 5.567 ± 0.056	6.048GlyThr: 6.048 ± 0.056	7.464GlyVal: 7.464 ± 0.066	1.714GlyTrp: 1.714 ± 0.028	2.282GlyTyr: 2.282 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	2.698HisAla: 2.698 ± 0.035	0.226HisCys: 0.226 ± 0.01	1.351HisAsp: 1.351 ± 0.025	1.269HisGlu: 1.269 ± 0.022	0.656HisPhe: 0.656 ± 0.017	2.335HisGly: 2.335 ± 0.031	0.655HisHis: 0.655 ± 0.017	0.753HisIle: 0.753 ± 0.018	0.441HisLys: 0.441 ± 0.014	2.311HisLeu: 2.311 ± 0.034	0.344HisMet: 0.344 ± 0.013	0.371HisAsn: 0.371 ± 0.013	1.731HisPro: 1.731 ± 0.028	0.677HisGln: 0.677 ± 0.017	2.042HisArg: 2.042 ± 0.031	1.028HisSer: 1.028 ± 0.019	1.311HisThr: 1.311 ± 0.022	1.633HisVal: 1.633 ± 0.027	0.379HisTrp: 0.379 ± 0.014	0.478HisTyr: 0.478 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	5.154IleAla: 5.154 ± 0.053	0.321IleCys: 0.321 ± 0.011	2.351IleAsp: 2.351 ± 0.031	2.111IleGlu: 2.111 ± 0.028	0.766IlePhe: 0.766 ± 0.018	3.923IleGly: 3.923 ± 0.047	0.646IleHis: 0.646 ± 0.018	0.93IleIle: 0.93 ± 0.02	0.883IleLys: 0.883 ± 0.02	2.537IleLeu: 2.537 ± 0.036	0.451IleMet: 0.451 ± 0.013	0.748IleAsn: 0.748 ± 0.017	1.882IlePro: 1.882 ± 0.027	0.772IleGln: 0.772 ± 0.018	2.263IleArg: 2.263 ± 0.032	1.844IleSer: 1.844 ± 0.027	2.359IleThr: 2.359 ± 0.031	2.811IleVal: 2.811 ± 0.035	0.409IleTrp: 0.409 ± 0.013	0.558IleTyr: 0.558 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	3.378LysAla: 3.378 ± 0.047	0.148LysCys: 0.148 ± 0.008	1.636LysAsp: 1.636 ± 0.031	1.445LysGlu: 1.445 ± 0.028	0.544LysPhe: 0.544 ± 0.015	2.251LysGly: 2.251 ± 0.037	0.499LysHis: 0.499 ± 0.017	1.021LysIle: 1.021 ± 0.021	1.203LysLys: 1.203 ± 0.038	2.335LysLeu: 2.335 ± 0.033	0.47LysMet: 0.47 ± 0.015	0.618LysAsn: 0.618 ± 0.018	1.615LysPro: 1.615 ± 0.03	0.826LysGln: 0.826 ± 0.019	1.641LysArg: 1.641 ± 0.028	1.491LysSer: 1.491 ± 0.024	1.554LysThr: 1.554 ± 0.034	2.156LysVal: 2.156 ± 0.033	0.333LysTrp: 0.333 ± 0.015	0.551LysTyr: 0.551 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	14.662LeuAla: 14.662 ± 0.107	0.881LeuCys: 0.881 ± 0.019	6.557LeuAsp: 6.557 ± 0.056	4.665LeuGlu: 4.665 ± 0.052	2.635LeuPhe: 2.635 ± 0.036	9.175LeuGly: 9.175 ± 0.083	2.316LeuHis: 2.316 ± 0.035	3.568LeuIle: 3.568 ± 0.044	2.301LeuLys: 2.301 ± 0.034	11.052LeuLeu: 11.052 ± 0.094	1.668LeuMet: 1.668 ± 0.026	1.782LeuAsn: 1.782 ± 0.027	6.351LeuPro: 6.351 ± 0.06	2.187LeuGln: 2.187 ± 0.029	8.419LeuArg: 8.419 ± 0.076	5.451LeuSer: 5.451 ± 0.05	6.798LeuThr: 6.798 ± 0.055	8.413LeuVal: 8.413 ± 0.073	1.272LeuTrp: 1.272 ± 0.023	1.836LeuTyr: 1.836 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	2.316MetAla: 2.316 ± 0.033	0.142MetCys: 0.142 ± 0.007	0.95MetAsp: 0.95 ± 0.019	0.793MetGlu: 0.793 ± 0.019	0.49MetPhe: 0.49 ± 0.013	1.395MetGly: 1.395 ± 0.022	0.364MetHis: 0.364 ± 0.012	0.703MetIle: 0.703 ± 0.015	0.492MetLys: 0.492 ± 0.014	1.715MetLeu: 1.715 ± 0.028	0.329MetMet: 0.329 ± 0.011	0.464MetAsn: 0.464 ± 0.012	1.191MetPro: 1.191 ± 0.021	0.446MetGln: 0.446 ± 0.016	1.455MetArg: 1.455 ± 0.021	1.364MetSer: 1.364 ± 0.023	1.536MetThr: 1.536 ± 0.024	1.296MetVal: 1.296 ± 0.024	0.222MetTrp: 0.222 ± 0.009	0.346MetTyr: 0.346 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.177AsnAla: 2.177 ± 0.032	0.185AsnCys: 0.185 ± 0.009	1.001AsnAsp: 1.001 ± 0.02	0.851AsnGlu: 0.851 ± 0.021	0.514AsnPhe: 0.514 ± 0.016	1.897AsnGly: 1.897 ± 0.031	0.409AsnHis: 0.409 ± 0.014	0.697AsnIle: 0.697 ± 0.018	0.508AsnLys: 0.508 ± 0.016	1.694AsnLeu: 1.694 ± 0.028	0.318AsnMet: 0.318 ± 0.011	0.453AsnAsn: 0.453 ± 0.014	1.367AsnPro: 1.367 ± 0.027	0.533AsnGln: 0.533 ± 0.017	1.23AsnArg: 1.23 ± 0.022	1.006AsnSer: 1.006 ± 0.023	1.11AsnThr: 1.11 ± 0.024	1.403AsnVal: 1.403 ± 0.026	0.311AsnTrp: 0.311 ± 0.01	0.42AsnTyr: 0.42 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	7.902ProAla: 7.902 ± 0.068	0.355ProCys: 0.355 ± 0.012	4.485ProAsp: 4.485 ± 0.049	4.323ProGlu: 4.323 ± 0.05	1.545ProPhe: 1.545 ± 0.024	6.333ProGly: 6.333 ± 0.06	1.41ProHis: 1.41 ± 0.023	1.387ProIle: 1.387 ± 0.021	1.47ProLys: 1.47 ± 0.026	5.307ProLeu: 5.307 ± 0.05	1.031ProMet: 1.031 ± 0.019	0.958ProAsn: 0.958 ± 0.019	3.346ProPro: 3.346 ± 0.045	1.883ProGln: 1.883 ± 0.031	3.858ProArg: 3.858 ± 0.043	3.199ProSer: 3.199 ± 0.045	3.122ProThr: 3.122 ± 0.037	5.162ProVal: 5.162 ± 0.044	0.871ProTrp: 0.871 ± 0.019	1.4ProTyr: 1.4 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.711GlnAla: 3.711 ± 0.044	0.184GlnCys: 0.184 ± 0.009	1.421GlnAsp: 1.421 ± 0.026	1.494GlnGlu: 1.494 ± 0.025	0.717GlnPhe: 0.717 ± 0.014	2.418GlnGly: 2.418 ± 0.029	0.65GlnHis: 0.65 ± 0.014	1.131GlnIle: 1.131 ± 0.023	0.699GlnLys: 0.699 ± 0.018	3.272GlnLeu: 3.272 ± 0.038	0.528GlnMet: 0.528 ± 0.015	0.532GlnAsn: 0.532 ± 0.014	1.721GlnPro: 1.721 ± 0.035	1.307GlnGln: 1.307 ± 0.033	2.402GlnArg: 2.402 ± 0.028	1.367GlnSer: 1.367 ± 0.022	1.39GlnThr: 1.39 ± 0.023	2.281GlnVal: 2.281 ± 0.036	0.482GlnTrp: 0.482 ± 0.013	0.627GlnTyr: 0.627 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	9.45ArgAla: 9.45 ± 0.081	0.599ArgCys: 0.599 ± 0.016	4.249ArgAsp: 4.249 ± 0.049	4.743ArgGlu: 4.743 ± 0.05	2.263ArgPhe: 2.263 ± 0.028	5.809ArgGly: 5.809 ± 0.052	2.103ArgHis: 2.103 ± 0.031	3.289ArgIle: 3.289 ± 0.033	1.845ArgLys: 1.845 ± 0.029	8.29ArgLeu: 8.29 ± 0.075	1.737ArgMet: 1.737 ± 0.024	1.315ArgAsn: 1.315 ± 0.025	4.552ArgPro: 4.552 ± 0.056	2.33ArgGln: 2.33 ± 0.032	7.361ArgArg: 7.361 ± 0.069	3.961ArgSer: 3.961 ± 0.044	5.252ArgThr: 5.252 ± 0.052	5.596ArgVal: 5.596 ± 0.056	1.365ArgTrp: 1.365 ± 0.023	1.728ArgTyr: 1.728 ± 0.024	0.0ArgXaa: 0.0 ± 0.0
Ser	6.901SerAla: 6.901 ± 0.061	0.426SerCys: 0.426 ± 0.012	2.859SerAsp: 2.859 ± 0.042	2.63SerGlu: 2.63 ± 0.039	1.557SerPhe: 1.557 ± 0.023	6.111SerGly: 6.111 ± 0.057	1.05SerHis: 1.05 ± 0.022	1.562SerIle: 1.562 ± 0.025	1.299SerLys: 1.299 ± 0.027	4.969SerLeu: 4.969 ± 0.046	1.153SerMet: 1.153 ± 0.023	0.904SerAsn: 0.904 ± 0.018	3.218SerPro: 3.218 ± 0.037	1.297SerGln: 1.297 ± 0.025	3.705SerArg: 3.705 ± 0.038	2.96SerSer: 2.96 ± 0.04	3.131SerThr: 3.131 ± 0.042	4.362SerVal: 4.362 ± 0.04	0.931SerTrp: 0.931 ± 0.021	1.274SerTyr: 1.274 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	8.492ThrAla: 8.492 ± 0.061	0.423ThrCys: 0.423 ± 0.014	3.659ThrAsp: 3.659 ± 0.04	3.319ThrGlu: 3.319 ± 0.037	1.639ThrPhe: 1.639 ± 0.026	6.431ThrGly: 6.431 ± 0.058	1.223ThrHis: 1.223 ± 0.023	1.782ThrIle: 1.782 ± 0.03	1.429ThrLys: 1.429 ± 0.029	5.685ThrLeu: 5.685 ± 0.053	0.982ThrMet: 0.982 ± 0.021	1.026ThrAsn: 1.026 ± 0.021	4.058ThrPro: 4.058 ± 0.046	1.409ThrGln: 1.409 ± 0.027	3.753ThrArg: 3.753 ± 0.04	3.226ThrSer: 3.226 ± 0.041	3.664ThrThr: 3.664 ± 0.045	5.813ThrVal: 5.813 ± 0.052	0.895ThrTrp: 0.895 ± 0.02	1.434ThrTyr: 1.434 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	10.383ValAla: 10.383 ± 0.066	0.743ValCys: 0.743 ± 0.017	4.914ValAsp: 4.914 ± 0.051	4.659ValGlu: 4.659 ± 0.047	2.348ValPhe: 2.348 ± 0.032	6.42ValGly: 6.42 ± 0.061	1.89ValHis: 1.89 ± 0.031	3.056ValIle: 3.056 ± 0.034	1.958ValLys: 1.958 ± 0.037	9.081ValLeu: 9.081 ± 0.079	1.427ValMet: 1.427 ± 0.028	1.604ValAsn: 1.604 ± 0.026	5.0ValPro: 5.0 ± 0.051	2.074ValGln: 2.074 ± 0.028	6.883ValArg: 6.883 ± 0.062	4.346ValSer: 4.346 ± 0.041	5.479ValThr: 5.479 ± 0.047	7.529ValVal: 7.529 ± 0.083	1.139ValTrp: 1.139 ± 0.02	1.544ValTyr: 1.544 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.672TrpAla: 1.672 ± 0.027	0.174TrpCys: 0.174 ± 0.008	0.857TrpAsp: 0.857 ± 0.02	0.754TrpGlu: 0.754 ± 0.018	0.508TrpPhe: 0.508 ± 0.014	1.103TrpGly: 1.103 ± 0.018	0.376TrpHis: 0.376 ± 0.012	0.569TrpIle: 0.569 ± 0.013	0.405TrpLys: 0.405 ± 0.014	1.776TrpLeu: 1.776 ± 0.029	0.307TrpMet: 0.307 ± 0.012	0.41TrpAsn: 0.41 ± 0.013	0.792TrpPro: 0.792 ± 0.02	0.639TrpGln: 0.639 ± 0.015	1.302TrpArg: 1.302 ± 0.025	0.956TrpSer: 0.956 ± 0.017	1.02TrpThr: 1.02 ± 0.022	0.995TrpVal: 0.995 ± 0.018	0.347TrpTrp: 0.347 ± 0.012	0.363TrpTyr: 0.363 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.908TyrAla: 2.908 ± 0.035	0.184TyrCys: 0.184 ± 0.009	1.528TyrAsp: 1.528 ± 0.031	1.425TyrGlu: 1.425 ± 0.022	0.668TyrPhe: 0.668 ± 0.017	2.361TyrGly: 2.361 ± 0.038	0.367TyrHis: 0.367 ± 0.011	0.513TyrIle: 0.513 ± 0.015	0.492TyrLys: 0.492 ± 0.013	2.028TyrLeu: 2.028 ± 0.032	0.251TyrMet: 0.251 ± 0.009	0.403TyrAsn: 0.403 ± 0.014	1.034TyrPro: 1.034 ± 0.022	0.61TyrGln: 0.61 ± 0.016	1.79TyrArg: 1.79 ± 0.027	0.982TyrSer: 0.982 ± 0.019	1.171TyrThr: 1.171 ± 0.024	1.71TyrVal: 1.71 ± 0.028	0.362TyrTrp: 0.362 ± 0.012	0.444TyrTyr: 0.444 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8167 proteins (2593571 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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