Lactococcus phage PLgY-16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; unclassified Skunavirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2P8G4|A0A2Z2P8G4_9CAUD Uncharacterized protein OS=Lactococcus phage PLgY-16 OX=1983589 PE=4 SV=1
MM1 pKa = 7.82KK2 pKa = 10.16DD3 pKa = 3.94FLMMWLFGTVLSLIAIAIAVFICVVIWIIVSEE35 pKa = 4.18LGIWTCFIIVPVIFGLMLAVTEE57 pKa = 4.29LL58 pKa = 3.53

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2P3H3|A0A2Z2P3H3_9CAUD Receptor binding protein OS=Lactococcus phage PLgY-16 OX=1983589 PE=4 SV=2
MM1 pKa = 7.72PLSKK5 pKa = 10.47EE6 pKa = 3.93LDD8 pKa = 3.72VKK10 pKa = 10.89SVSSFFEE17 pKa = 4.18KK18 pKa = 10.36EE19 pKa = 3.67VTMKK23 pKa = 10.65VSDD26 pKa = 4.07FVQRR30 pKa = 11.84DD31 pKa = 3.63VQKK34 pKa = 10.18RR35 pKa = 11.84AVRR38 pKa = 11.84AGRR41 pKa = 11.84RR42 pKa = 11.84PKK44 pKa = 10.42RR45 pKa = 11.84VWNRR49 pKa = 11.84HH50 pKa = 3.69SGKK53 pKa = 8.96TYY55 pKa = 10.16RR56 pKa = 11.84YY57 pKa = 7.67TNTGQLGRR65 pKa = 11.84NIKK68 pKa = 10.0KK69 pKa = 9.23IRR71 pKa = 11.84SGEE74 pKa = 4.23GYY76 pKa = 10.34KK77 pKa = 9.71IDD79 pKa = 3.57AGTRR83 pKa = 11.84GNYY86 pKa = 5.24THH88 pKa = 7.61GYY90 pKa = 7.83HH91 pKa = 6.79GLYY94 pKa = 10.45FLVEE98 pKa = 4.18RR99 pKa = 11.84QGEE102 pKa = 4.09RR103 pKa = 11.84DD104 pKa = 3.41VRR106 pKa = 11.84STLNATKK113 pKa = 10.22KK114 pKa = 9.81YY115 pKa = 11.0AEE117 pKa = 4.13ALKK120 pKa = 10.92VV121 pKa = 3.35

Molecular weight: 14.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

8778

49

995

159.6

18.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.425 ± 0.482

0.627 ± 0.157

5.719 ± 0.247

7.735 ± 0.605

4.454 ± 0.283

6.004 ± 0.41

1.151 ± 0.172

7.302 ± 0.347

8.852 ± 0.48

7.724 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.848 ± 0.152

6.049 ± 0.26

2.518 ± 0.247

3.68 ± 0.226

3.748 ± 0.319

6.129 ± 0.392

6.505 ± 0.337

6.699 ± 0.387

1.629 ± 0.212

4.204 ± 0.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski