Amino acid dipepetide frequency for Lactococcus phage PLgY-16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.57AlaAla: 0.57 ± 0.3
0.228AlaCys: 0.228 ± 0.172
3.304AlaAsp: 3.304 ± 0.59
4.785AlaGlu: 4.785 ± 1.261
2.734AlaPhe: 2.734 ± 0.596
3.418AlaGly: 3.418 ± 0.903
0.798AlaHis: 0.798 ± 0.352
6.836AlaIle: 6.836 ± 1.185
4.785AlaLys: 4.785 ± 0.777
5.925AlaLeu: 5.925 ± 0.852
2.507AlaMet: 2.507 ± 0.746
3.532AlaAsn: 3.532 ± 0.511
0.684AlaPro: 0.684 ± 0.245
2.165AlaGln: 2.165 ± 0.469
3.874AlaArg: 3.874 ± 0.626
5.925AlaSer: 5.925 ± 1.239
3.532AlaThr: 3.532 ± 0.831
4.899AlaVal: 4.899 ± 0.824
1.367AlaTrp: 1.367 ± 0.426
2.393AlaTyr: 2.393 ± 0.661
0.0AlaXaa: 0.0 ± 0.0
Cys
0.342CysAla: 0.342 ± 0.241
0.114CysCys: 0.114 ± 0.11
0.456CysAsp: 0.456 ± 0.212
0.456CysGlu: 0.456 ± 0.222
0.342CysPhe: 0.342 ± 0.195
0.342CysGly: 0.342 ± 0.207
0.114CysHis: 0.114 ± 0.115
0.342CysIle: 0.342 ± 0.177
0.684CysLys: 0.684 ± 0.277
0.228CysLeu: 0.228 ± 0.162
0.0CysMet: 0.0 ± 0.0
0.342CysAsn: 0.342 ± 0.194
0.114CysPro: 0.114 ± 0.118
0.228CysGln: 0.228 ± 0.158
0.228CysArg: 0.228 ± 0.163
0.684CysSer: 0.684 ± 0.272
0.114CysThr: 0.114 ± 0.11
0.798CysVal: 0.798 ± 0.293
0.0CysTrp: 0.0 ± 0.0
0.342CysTyr: 0.342 ± 0.196
0.0CysXaa: 0.0 ± 0.0
Asp
2.848AspAla: 2.848 ± 0.592
0.228AspCys: 0.228 ± 0.177
3.304AspAsp: 3.304 ± 0.694
4.785AspGlu: 4.785 ± 0.807
3.19AspPhe: 3.19 ± 0.627
2.62AspGly: 2.62 ± 0.56
0.456AspHis: 0.456 ± 0.264
4.671AspIle: 4.671 ± 0.609
4.329AspLys: 4.329 ± 0.666
5.013AspLeu: 5.013 ± 0.635
1.709AspMet: 1.709 ± 0.396
4.785AspAsn: 4.785 ± 0.793
1.595AspPro: 1.595 ± 0.505
0.911AspGln: 0.911 ± 0.376
2.051AspArg: 2.051 ± 0.541
2.734AspSer: 2.734 ± 0.533
4.671AspThr: 4.671 ± 0.614
3.988AspVal: 3.988 ± 0.664
1.139AspTrp: 1.139 ± 0.323
2.165AspTyr: 2.165 ± 0.634
0.0AspXaa: 0.0 ± 0.0
Glu
4.216GluAla: 4.216 ± 0.843
0.342GluCys: 0.342 ± 0.211
4.557GluAsp: 4.557 ± 0.861
5.127GluGlu: 5.127 ± 0.961
3.76GluPhe: 3.76 ± 0.61
3.532GluGly: 3.532 ± 0.485
0.57GluHis: 0.57 ± 0.271
5.811GluIle: 5.811 ± 0.785
7.064GluLys: 7.064 ± 1.367
7.292GluLeu: 7.292 ± 1.095
2.393GluMet: 2.393 ± 0.514
5.013GluAsn: 5.013 ± 0.685
2.165GluPro: 2.165 ± 0.566
4.443GluGln: 4.443 ± 0.813
2.62GluArg: 2.62 ± 0.604
3.304GluSer: 3.304 ± 0.685
4.443GluThr: 4.443 ± 0.792
5.355GluVal: 5.355 ± 0.829
1.253GluTrp: 1.253 ± 0.462
4.102GluTyr: 4.102 ± 0.989
0.0GluXaa: 0.0 ± 0.0
Phe
2.165PheAla: 2.165 ± 0.642
0.342PheCys: 0.342 ± 0.195
2.393PheAsp: 2.393 ± 0.507
3.532PheGlu: 3.532 ± 0.572
2.734PhePhe: 2.734 ± 0.907
2.962PheGly: 2.962 ± 0.625
0.114PheHis: 0.114 ± 0.111
3.646PheIle: 3.646 ± 0.695
3.988PheLys: 3.988 ± 0.568
2.62PheLeu: 2.62 ± 0.459
1.481PheMet: 1.481 ± 0.454
2.962PheAsn: 2.962 ± 0.763
1.139PhePro: 1.139 ± 0.411
1.595PheGln: 1.595 ± 0.426
1.139PheArg: 1.139 ± 0.42
3.532PheSer: 3.532 ± 0.937
3.19PheThr: 3.19 ± 0.535
2.962PheVal: 2.962 ± 0.533
0.57PheTrp: 0.57 ± 0.244
1.481PheTyr: 1.481 ± 0.397
0.0PheXaa: 0.0 ± 0.0
Gly
4.102GlyAla: 4.102 ± 0.71
0.798GlyCys: 0.798 ± 0.296
2.962GlyAsp: 2.962 ± 0.57
4.671GlyGlu: 4.671 ± 0.839
2.165GlyPhe: 2.165 ± 0.53
3.988GlyGly: 3.988 ± 0.717
0.342GlyHis: 0.342 ± 0.194
4.671GlyIle: 4.671 ± 1.432
5.127GlyLys: 5.127 ± 0.936
6.039GlyLeu: 6.039 ± 0.703
1.367GlyMet: 1.367 ± 0.507
3.076GlyAsn: 3.076 ± 0.519
0.114GlyPro: 0.114 ± 0.129
2.165GlyGln: 2.165 ± 0.537
2.507GlyArg: 2.507 ± 0.505
4.216GlySer: 4.216 ± 0.601
3.646GlyThr: 3.646 ± 0.717
3.418GlyVal: 3.418 ± 0.674
1.025GlyTrp: 1.025 ± 0.46
3.646GlyTyr: 3.646 ± 0.796
0.0GlyXaa: 0.0 ± 0.0
His
0.684HisAla: 0.684 ± 0.283
0.228HisCys: 0.228 ± 0.164
0.57HisAsp: 0.57 ± 0.252
0.57HisGlu: 0.57 ± 0.257
0.342HisPhe: 0.342 ± 0.196
1.367HisGly: 1.367 ± 0.444
0.456HisHis: 0.456 ± 0.301
1.025HisIle: 1.025 ± 0.33
1.253HisLys: 1.253 ± 0.503
0.911HisLeu: 0.911 ± 0.33
0.456HisMet: 0.456 ± 0.218
0.342HisAsn: 0.342 ± 0.251
0.342HisPro: 0.342 ± 0.202
0.342HisGln: 0.342 ± 0.182
0.342HisArg: 0.342 ± 0.227
0.228HisSer: 0.228 ± 0.16
0.57HisThr: 0.57 ± 0.259
1.025HisVal: 1.025 ± 0.445
0.114HisTrp: 0.114 ± 0.117
0.342HisTyr: 0.342 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
5.469IleAla: 5.469 ± 0.87
0.228IleCys: 0.228 ± 0.174
5.811IleAsp: 5.811 ± 0.662
7.748IleGlu: 7.748 ± 0.921
2.734IlePhe: 2.734 ± 0.452
4.557IleGly: 4.557 ± 1.001
0.798IleHis: 0.798 ± 0.353
3.76IleIle: 3.76 ± 0.776
6.152IleLys: 6.152 ± 0.874
4.216IleLeu: 4.216 ± 0.926
1.823IleMet: 1.823 ± 0.378
3.874IleAsn: 3.874 ± 0.6
1.367IlePro: 1.367 ± 0.366
2.62IleGln: 2.62 ± 0.651
1.937IleArg: 1.937 ± 0.5
4.216IleSer: 4.216 ± 0.928
4.557IleThr: 4.557 ± 0.852
5.925IleVal: 5.925 ± 1.093
2.165IleTrp: 2.165 ± 1.033
3.076IleTyr: 3.076 ± 0.678
0.0IleXaa: 0.0 ± 0.0
Lys
6.836LysAla: 6.836 ± 0.964
0.228LysCys: 0.228 ± 0.154
4.785LysAsp: 4.785 ± 0.887
7.975LysGlu: 7.975 ± 1.26
2.734LysPhe: 2.734 ± 0.457
5.127LysGly: 5.127 ± 0.855
1.025LysHis: 1.025 ± 0.356
5.583LysIle: 5.583 ± 0.79
9.57LysLys: 9.57 ± 1.377
6.836LysLeu: 6.836 ± 1.131
2.62LysMet: 2.62 ± 0.521
4.899LysAsn: 4.899 ± 0.705
2.393LysPro: 2.393 ± 0.584
3.418LysGln: 3.418 ± 0.656
4.329LysArg: 4.329 ± 0.703
4.216LysSer: 4.216 ± 0.579
6.266LysThr: 6.266 ± 0.773
5.127LysVal: 5.127 ± 0.849
1.595LysTrp: 1.595 ± 0.306
2.962LysTyr: 2.962 ± 0.612
0.0LysXaa: 0.0 ± 0.0
Leu
4.329LeuAla: 4.329 ± 0.603
0.456LeuCys: 0.456 ± 0.243
4.443LeuAsp: 4.443 ± 0.792
6.039LeuGlu: 6.039 ± 0.837
2.962LeuPhe: 2.962 ± 0.849
4.216LeuGly: 4.216 ± 0.568
1.253LeuHis: 1.253 ± 0.401
5.013LeuIle: 5.013 ± 0.98
7.975LeuLys: 7.975 ± 1.182
4.443LeuLeu: 4.443 ± 0.702
2.051LeuMet: 2.051 ± 0.496
5.013LeuAsn: 5.013 ± 0.71
2.507LeuPro: 2.507 ± 0.492
3.076LeuGln: 3.076 ± 0.529
1.709LeuArg: 1.709 ± 0.331
5.013LeuSer: 5.013 ± 0.877
5.469LeuThr: 5.469 ± 0.739
6.836LeuVal: 6.836 ± 0.974
1.139LeuTrp: 1.139 ± 0.417
3.304LeuTyr: 3.304 ± 0.712
0.0LeuXaa: 0.0 ± 0.0
Met
2.734MetAla: 2.734 ± 0.486
0.342MetCys: 0.342 ± 0.193
1.367MetAsp: 1.367 ± 0.4
2.051MetGlu: 2.051 ± 0.548
0.911MetPhe: 0.911 ± 0.371
1.367MetGly: 1.367 ± 0.405
0.0MetHis: 0.0 ± 0.0
2.848MetIle: 2.848 ± 0.631
3.19MetLys: 3.19 ± 0.623
1.937MetLeu: 1.937 ± 0.58
0.342MetMet: 0.342 ± 0.197
1.937MetAsn: 1.937 ± 0.445
0.342MetPro: 0.342 ± 0.155
0.911MetGln: 0.911 ± 0.336
1.253MetArg: 1.253 ± 0.349
1.253MetSer: 1.253 ± 0.389
3.076MetThr: 3.076 ± 0.515
1.595MetVal: 1.595 ± 0.496
0.456MetTrp: 0.456 ± 0.221
0.57MetTyr: 0.57 ± 0.206
0.0MetXaa: 0.0 ± 0.0
Asn
4.557AsnAla: 4.557 ± 0.942
0.228AsnCys: 0.228 ± 0.179
2.848AsnAsp: 2.848 ± 0.58
5.697AsnGlu: 5.697 ± 0.797
2.962AsnPhe: 2.962 ± 0.575
4.329AsnGly: 4.329 ± 0.612
0.57AsnHis: 0.57 ± 0.237
4.785AsnIle: 4.785 ± 0.963
5.241AsnLys: 5.241 ± 0.833
4.671AsnLeu: 4.671 ± 0.696
1.367AsnMet: 1.367 ± 0.31
3.304AsnAsn: 3.304 ± 0.672
2.393AsnPro: 2.393 ± 0.448
2.507AsnGln: 2.507 ± 0.561
1.253AsnArg: 1.253 ± 0.465
3.532AsnSer: 3.532 ± 0.633
2.962AsnThr: 2.962 ± 0.741
3.076AsnVal: 3.076 ± 0.48
1.139AsnTrp: 1.139 ± 0.444
3.076AsnTyr: 3.076 ± 0.754
0.0AsnXaa: 0.0 ± 0.0
Pro
1.937ProAla: 1.937 ± 0.493
0.228ProCys: 0.228 ± 0.169
1.481ProAsp: 1.481 ± 0.667
2.62ProGlu: 2.62 ± 0.548
1.595ProPhe: 1.595 ± 0.377
0.57ProGly: 0.57 ± 0.239
0.0ProHis: 0.0 ± 0.0
1.481ProIle: 1.481 ± 0.418
2.279ProLys: 2.279 ± 0.64
2.393ProLeu: 2.393 ± 0.554
1.025ProMet: 1.025 ± 0.338
0.911ProAsn: 0.911 ± 0.39
0.456ProPro: 0.456 ± 0.223
0.798ProGln: 0.798 ± 0.313
0.798ProArg: 0.798 ± 0.282
1.937ProSer: 1.937 ± 0.427
2.165ProThr: 2.165 ± 0.601
1.823ProVal: 1.823 ± 0.478
0.342ProTrp: 0.342 ± 0.21
0.342ProTyr: 0.342 ± 0.207
0.0ProXaa: 0.0 ± 0.0
Gln
2.962GlnAla: 2.962 ± 0.504
0.114GlnCys: 0.114 ± 0.118
1.709GlnAsp: 1.709 ± 0.464
2.62GlnGlu: 2.62 ± 0.585
1.937GlnPhe: 1.937 ± 0.379
2.165GlnGly: 2.165 ± 0.421
0.228GlnHis: 0.228 ± 0.192
1.595GlnIle: 1.595 ± 0.399
3.76GlnLys: 3.76 ± 0.876
3.76GlnLeu: 3.76 ± 0.621
1.481GlnMet: 1.481 ± 0.503
1.139GlnAsn: 1.139 ± 0.385
1.367GlnPro: 1.367 ± 0.394
1.025GlnGln: 1.025 ± 0.351
1.823GlnArg: 1.823 ± 0.432
2.507GlnSer: 2.507 ± 0.442
2.279GlnThr: 2.279 ± 0.585
1.937GlnVal: 1.937 ± 0.518
0.456GlnTrp: 0.456 ± 0.217
1.937GlnTyr: 1.937 ± 0.447
0.0GlnXaa: 0.0 ± 0.0
Arg
3.19ArgAla: 3.19 ± 0.629
0.114ArgCys: 0.114 ± 0.122
2.507ArgAsp: 2.507 ± 0.623
2.62ArgGlu: 2.62 ± 0.584
0.798ArgPhe: 0.798 ± 0.283
1.823ArgGly: 1.823 ± 0.432
1.367ArgHis: 1.367 ± 0.465
1.709ArgIle: 1.709 ± 0.458
3.646ArgLys: 3.646 ± 0.649
2.62ArgLeu: 2.62 ± 0.501
0.911ArgMet: 0.911 ± 0.251
2.393ArgAsn: 2.393 ± 0.534
1.025ArgPro: 1.025 ± 0.386
1.481ArgGln: 1.481 ± 0.407
1.937ArgArg: 1.937 ± 0.501
1.595ArgSer: 1.595 ± 0.365
1.595ArgThr: 1.595 ± 0.507
2.507ArgVal: 2.507 ± 0.523
0.456ArgTrp: 0.456 ± 0.209
3.19ArgTyr: 3.19 ± 0.75
0.0ArgXaa: 0.0 ± 0.0
Ser
4.216SerAla: 4.216 ± 1.155
0.456SerCys: 0.456 ± 0.239
2.962SerAsp: 2.962 ± 0.529
3.988SerGlu: 3.988 ± 0.671
3.418SerPhe: 3.418 ± 0.642
5.469SerGly: 5.469 ± 1.022
0.57SerHis: 0.57 ± 0.233
4.216SerIle: 4.216 ± 0.75
4.671SerLys: 4.671 ± 0.918
4.899SerLeu: 4.899 ± 0.696
2.279SerMet: 2.279 ± 0.477
3.076SerAsn: 3.076 ± 0.606
1.823SerPro: 1.823 ± 0.317
2.165SerGln: 2.165 ± 0.551
1.823SerArg: 1.823 ± 0.437
3.988SerSer: 3.988 ± 0.643
3.304SerThr: 3.304 ± 0.587
5.013SerVal: 5.013 ± 0.576
0.684SerTrp: 0.684 ± 0.285
2.279SerTyr: 2.279 ± 0.65
0.0SerXaa: 0.0 ± 0.0
Thr
5.583ThrAla: 5.583 ± 0.99
0.456ThrCys: 0.456 ± 0.239
4.443ThrAsp: 4.443 ± 0.701
3.304ThrGlu: 3.304 ± 0.686
2.962ThrPhe: 2.962 ± 0.744
5.355ThrGly: 5.355 ± 0.892
0.911ThrHis: 0.911 ± 0.309
5.241ThrIle: 5.241 ± 0.754
4.557ThrLys: 4.557 ± 0.966
4.557ThrLeu: 4.557 ± 0.847
1.481ThrMet: 1.481 ± 0.387
3.874ThrAsn: 3.874 ± 0.593
1.709ThrPro: 1.709 ± 0.576
2.734ThrGln: 2.734 ± 0.49
2.393ThrArg: 2.393 ± 0.676
4.443ThrSer: 4.443 ± 0.687
4.102ThrThr: 4.102 ± 0.876
3.646ThrVal: 3.646 ± 0.565
1.139ThrTrp: 1.139 ± 0.413
2.165ThrTyr: 2.165 ± 0.431
0.0ThrXaa: 0.0 ± 0.0
Val
4.102ValAla: 4.102 ± 0.639
0.456ValCys: 0.456 ± 0.268
3.874ValAsp: 3.874 ± 0.677
4.557ValGlu: 4.557 ± 0.756
3.418ValPhe: 3.418 ± 0.866
3.304ValGly: 3.304 ± 0.613
0.684ValHis: 0.684 ± 0.37
5.241ValIle: 5.241 ± 0.788
5.925ValLys: 5.925 ± 0.839
3.874ValLeu: 3.874 ± 0.638
1.709ValMet: 1.709 ± 0.381
4.557ValAsn: 4.557 ± 0.731
2.051ValPro: 2.051 ± 0.474
2.165ValGln: 2.165 ± 0.413
3.19ValArg: 3.19 ± 0.569
4.899ValSer: 4.899 ± 0.823
5.355ValThr: 5.355 ± 0.803
4.216ValVal: 4.216 ± 0.674
0.684ValTrp: 0.684 ± 0.295
2.734ValTyr: 2.734 ± 0.584
0.0ValXaa: 0.0 ± 0.0
Trp
1.025TrpAla: 1.025 ± 0.292
0.342TrpCys: 0.342 ± 0.199
0.798TrpAsp: 0.798 ± 0.33
0.684TrpGlu: 0.684 ± 0.295
1.253TrpPhe: 1.253 ± 0.502
0.57TrpGly: 0.57 ± 0.214
0.228TrpHis: 0.228 ± 0.163
0.684TrpIle: 0.684 ± 0.227
1.025TrpLys: 1.025 ± 0.3
1.595TrpLeu: 1.595 ± 0.421
0.342TrpMet: 0.342 ± 0.216
2.279TrpAsn: 2.279 ± 0.768
0.0TrpPro: 0.0 ± 0.0
0.911TrpGln: 0.911 ± 0.287
0.684TrpArg: 0.684 ± 0.405
1.139TrpSer: 1.139 ± 0.385
0.684TrpThr: 0.684 ± 0.265
1.253TrpVal: 1.253 ± 0.326
0.456TrpTrp: 0.456 ± 0.2
0.342TrpTyr: 0.342 ± 0.199
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.393TyrAla: 2.393 ± 0.53
0.342TyrCys: 0.342 ± 0.194
2.62TyrAsp: 2.62 ± 0.845
3.532TyrGlu: 3.532 ± 0.755
1.595TyrPhe: 1.595 ± 0.441
2.962TyrGly: 2.962 ± 0.67
1.025TyrHis: 1.025 ± 0.284
3.874TyrIle: 3.874 ± 0.837
3.304TyrLys: 3.304 ± 0.714
3.418TyrLeu: 3.418 ± 0.693
1.025TyrMet: 1.025 ± 0.409
3.19TyrAsn: 3.19 ± 0.607
1.595TyrPro: 1.595 ± 0.541
1.139TyrGln: 1.139 ± 0.5
1.595TyrArg: 1.595 ± 0.539
1.937TyrSer: 1.937 ± 0.518
3.076TyrThr: 3.076 ± 0.58
1.595TyrVal: 1.595 ± 0.522
0.114TyrTrp: 0.114 ± 0.129
1.595TyrTyr: 1.595 ± 0.44
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (8778 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski