Pseudomonas sp. SST3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6978 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A658G1C8|A0A658G1C8_9PSED DUF479 domain-containing protein (Fragment) OS=Pseudomonas sp. SST3 OX=2267882 GN=DQ405_47760 PE=4 SV=1
MM1 pKa = 7.32VGGEE5 pKa = 3.69AAGYY9 pKa = 8.21VAHH12 pKa = 7.66AAPQQVRR19 pKa = 11.84IPQSSADD26 pKa = 3.81WLWEE30 pKa = 3.55QDD32 pKa = 3.6VNHH35 pKa = 5.84YY36 pKa = 9.8PEE38 pKa = 3.79WSYY41 pKa = 11.26TMFNKK46 pKa = 8.71KK47 pKa = 7.57TLLALLISAAAANAMAASSEE67 pKa = 4.4AYY69 pKa = 10.25VDD71 pKa = 3.47QDD73 pKa = 4.45GNNQQATVTQEE84 pKa = 4.1GGDD87 pKa = 3.45QFALVFQDD95 pKa = 3.63GEE97 pKa = 4.69GNIGTTYY104 pKa = 10.6QAGSDD109 pKa = 3.8NLSGVYY115 pKa = 8.58QAGNMNVAAVTQSSTNSGSTVVQAGFANKK144 pKa = 10.6AEE146 pKa = 4.22VLQVATNNTSFVNQTGEE163 pKa = 3.93ANEE166 pKa = 4.06AFVTHH171 pKa = 6.81GGGGSHH177 pKa = 7.12FSEE180 pKa = 4.68VTQNGISNDD189 pKa = 3.25ATLKK193 pKa = 10.73QGGNGNWSFITQAGNDD209 pKa = 3.22NKK211 pKa = 11.28ANVGQYY217 pKa = 11.04GNNNLSDD224 pKa = 3.57VSQNGNDD231 pKa = 3.35NVATVSQKK239 pKa = 9.8DD240 pKa = 3.24TGMVYY245 pKa = 10.85ADD247 pKa = 4.0QIGTGNEE254 pKa = 3.97LLVTQGDD261 pKa = 3.99LAVAEE266 pKa = 4.19ILQTGEE272 pKa = 3.97FNDD275 pKa = 4.03VEE277 pKa = 4.33IEE279 pKa = 4.13QKK281 pKa = 11.16GSGNYY286 pKa = 10.54ANVDD290 pKa = 3.05QYY292 pKa = 11.84GQSNNTDD299 pKa = 2.89ITQSGTDD306 pKa = 3.04NDD308 pKa = 4.04AYY310 pKa = 9.75IVQGGVSNMAVAMQFNDD327 pKa = 3.19VV328 pKa = 3.48

Molecular weight: 34.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A658G6D3|A0A658G6D3_9PSED Flavin-nucleotide-binding protein OS=Pseudomonas sp. SST3 OX=2267882 GN=DQ405_47200 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.38GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6978

0

6978

1805302

20

2393

258.7

28.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.813 ± 0.036

1.046 ± 0.011

5.364 ± 0.025

6.013 ± 0.031

3.669 ± 0.02

7.885 ± 0.029

2.305 ± 0.016

4.914 ± 0.021

3.412 ± 0.023

11.629 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.016

2.956 ± 0.018

4.823 ± 0.022

4.428 ± 0.025

6.655 ± 0.031

5.777 ± 0.021

4.862 ± 0.02

7.034 ± 0.027

1.439 ± 0.016

2.526 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski