Amino acid dipepetide frequency for Pseudomonas sp. SST3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.31AlaAla: 12.31 ± 0.115
1.272AlaCys: 1.272 ± 0.028
5.749AlaAsp: 5.749 ± 0.051
7.226AlaGlu: 7.226 ± 0.08
3.921AlaPhe: 3.921 ± 0.051
8.748AlaGly: 8.748 ± 0.071
2.197AlaHis: 2.197 ± 0.037
5.343AlaIle: 5.343 ± 0.068
3.71AlaLys: 3.71 ± 0.05
13.179AlaLeu: 13.179 ± 0.104
3.008AlaMet: 3.008 ± 0.037
2.908AlaAsn: 2.908 ± 0.044
4.398AlaPro: 4.398 ± 0.047
4.804AlaGln: 4.804 ± 0.063
6.927AlaArg: 6.927 ± 0.066
6.077AlaSer: 6.077 ± 0.064
4.856AlaThr: 4.856 ± 0.058
7.464AlaVal: 7.464 ± 0.067
1.529AlaTrp: 1.529 ± 0.03
2.505AlaTyr: 2.505 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
1.082CysAla: 1.082 ± 0.031
0.172CysCys: 0.172 ± 0.011
0.559CysAsp: 0.559 ± 0.018
0.61CysGlu: 0.61 ± 0.017
0.359CysPhe: 0.359 ± 0.016
0.996CysGly: 0.996 ± 0.025
0.315CysHis: 0.315 ± 0.013
0.494CysIle: 0.494 ± 0.02
0.318CysLys: 0.318 ± 0.014
1.126CysLeu: 1.126 ± 0.024
0.222CysMet: 0.222 ± 0.011
0.271CysAsn: 0.271 ± 0.012
0.532CysPro: 0.532 ± 0.016
0.428CysGln: 0.428 ± 0.017
0.709CysArg: 0.709 ± 0.02
0.67CysSer: 0.67 ± 0.021
0.465CysThr: 0.465 ± 0.016
0.71CysVal: 0.71 ± 0.022
0.158CysTrp: 0.158 ± 0.01
0.267CysTyr: 0.267 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
5.678AspAla: 5.678 ± 0.06
0.533AspCys: 0.533 ± 0.018
2.918AspAsp: 2.918 ± 0.049
3.774AspGlu: 3.774 ± 0.05
2.111AspPhe: 2.111 ± 0.037
4.468AspGly: 4.468 ± 0.06
1.229AspHis: 1.229 ± 0.027
2.657AspIle: 2.657 ± 0.046
1.87AspLys: 1.87 ± 0.03
5.77AspLeu: 5.77 ± 0.06
1.288AspMet: 1.288 ± 0.027
1.512AspAsn: 1.512 ± 0.03
2.84AspPro: 2.84 ± 0.04
2.189AspGln: 2.189 ± 0.033
3.315AspArg: 3.315 ± 0.038
2.908AspSer: 2.908 ± 0.042
2.38AspThr: 2.38 ± 0.04
3.603AspVal: 3.603 ± 0.05
0.943AspTrp: 0.943 ± 0.025
1.655AspTyr: 1.655 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
6.658GluAla: 6.658 ± 0.067
0.489GluCys: 0.489 ± 0.018
2.725GluAsp: 2.725 ± 0.043
3.363GluGlu: 3.363 ± 0.059
1.877GluPhe: 1.877 ± 0.031
4.059GluGly: 4.059 ± 0.059
1.679GluHis: 1.679 ± 0.031
2.94GluIle: 2.94 ± 0.049
2.159GluLys: 2.159 ± 0.036
7.262GluLeu: 7.262 ± 0.084
1.496GluMet: 1.496 ± 0.031
1.647GluAsn: 1.647 ± 0.033
2.787GluPro: 2.787 ± 0.043
3.771GluGln: 3.771 ± 0.051
5.13GluArg: 5.13 ± 0.063
2.921GluSer: 2.921 ± 0.044
2.843GluThr: 2.843 ± 0.041
4.259GluVal: 4.259 ± 0.051
0.785GluTrp: 0.785 ± 0.02
1.277GluTyr: 1.277 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
4.101PheAla: 4.101 ± 0.049
0.484PheCys: 0.484 ± 0.016
2.444PheAsp: 2.444 ± 0.039
2.233PheGlu: 2.233 ± 0.03
1.5PhePhe: 1.5 ± 0.033
3.273PheGly: 3.273 ± 0.046
0.787PheHis: 0.787 ± 0.019
1.879PheIle: 1.879 ± 0.033
1.219PheLys: 1.219 ± 0.026
3.272PheLeu: 3.272 ± 0.044
0.851PheMet: 0.851 ± 0.026
1.328PheAsn: 1.328 ± 0.028
1.454PhePro: 1.454 ± 0.032
1.213PheGln: 1.213 ± 0.026
1.973PheArg: 1.973 ± 0.036
2.516PheSer: 2.516 ± 0.038
1.954PheThr: 1.954 ± 0.034
2.577PheVal: 2.577 ± 0.044
0.58PheTrp: 0.58 ± 0.019
1.047PheTyr: 1.047 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
7.186GlyAla: 7.186 ± 0.075
1.008GlyCys: 1.008 ± 0.026
3.99GlyAsp: 3.99 ± 0.049
4.935GlyGlu: 4.935 ± 0.058
3.307GlyPhe: 3.307 ± 0.04
5.917GlyGly: 5.917 ± 0.079
1.833GlyHis: 1.833 ± 0.034
4.209GlyIle: 4.209 ± 0.045
3.364GlyLys: 3.364 ± 0.048
9.241GlyLeu: 9.241 ± 0.092
2.381GlyMet: 2.381 ± 0.039
2.274GlyAsn: 2.274 ± 0.041
2.601GlyPro: 2.601 ± 0.041
3.388GlyGln: 3.388 ± 0.048
4.971GlyArg: 4.971 ± 0.059
4.603GlySer: 4.603 ± 0.052
3.886GlyThr: 3.886 ± 0.047
5.916GlyVal: 5.916 ± 0.073
1.377GlyTrp: 1.377 ± 0.027
2.468GlyTyr: 2.468 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
2.287HisAla: 2.287 ± 0.035
0.355HisCys: 0.355 ± 0.015
1.247HisAsp: 1.247 ± 0.029
1.306HisGlu: 1.306 ± 0.027
0.982HisPhe: 0.982 ± 0.024
2.051HisGly: 2.051 ± 0.036
0.668HisHis: 0.668 ± 0.021
1.048HisIle: 1.048 ± 0.025
0.63HisLys: 0.63 ± 0.02
2.597HisLeu: 2.597 ± 0.043
0.552HisMet: 0.552 ± 0.018
0.635HisAsn: 0.635 ± 0.019
1.425HisPro: 1.425 ± 0.032
0.984HisGln: 0.984 ± 0.025
1.446HisArg: 1.446 ± 0.028
1.27HisSer: 1.27 ± 0.03
1.007HisThr: 1.007 ± 0.022
1.362HisVal: 1.362 ± 0.027
0.455HisTrp: 0.455 ± 0.017
0.746HisTyr: 0.746 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.892IleAla: 5.892 ± 0.063
0.519IleCys: 0.519 ± 0.018
3.337IleAsp: 3.337 ± 0.043
3.599IleGlu: 3.599 ± 0.05
1.564IlePhe: 1.564 ± 0.033
4.62IleGly: 4.62 ± 0.057
1.033IleHis: 1.033 ± 0.028
2.229IleIle: 2.229 ± 0.042
1.758IleLys: 1.758 ± 0.034
4.203IleLeu: 4.203 ± 0.056
0.907IleMet: 0.907 ± 0.024
1.749IleAsn: 1.749 ± 0.034
2.283IlePro: 2.283 ± 0.036
1.721IleGln: 1.721 ± 0.032
3.084IleArg: 3.084 ± 0.042
3.069IleSer: 3.069 ± 0.041
2.56IleThr: 2.56 ± 0.038
3.235IleVal: 3.235 ± 0.041
0.554IleTrp: 0.554 ± 0.017
1.219IleTyr: 1.219 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
4.023LysAla: 4.023 ± 0.052
0.215LysCys: 0.215 ± 0.01
1.637LysAsp: 1.637 ± 0.031
1.721LysGlu: 1.721 ± 0.038
0.903LysPhe: 0.903 ± 0.022
2.631LysGly: 2.631 ± 0.034
0.852LysHis: 0.852 ± 0.02
1.621LysIle: 1.621 ± 0.033
1.304LysLys: 1.304 ± 0.03
3.78LysLeu: 3.78 ± 0.048
0.744LysMet: 0.744 ± 0.02
1.012LysAsn: 1.012 ± 0.027
2.086LysPro: 2.086 ± 0.039
1.631LysGln: 1.631 ± 0.03
2.561LysArg: 2.561 ± 0.041
1.829LysSer: 1.829 ± 0.032
1.882LysThr: 1.882 ± 0.035
2.608LysVal: 2.608 ± 0.047
0.398LysTrp: 0.398 ± 0.014
0.679LysTyr: 0.679 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
13.796LeuAla: 13.796 ± 0.102
1.228LeuCys: 1.228 ± 0.028
6.591LeuAsp: 6.591 ± 0.064
6.61LeuGlu: 6.61 ± 0.078
4.264LeuPhe: 4.264 ± 0.054
8.942LeuGly: 8.942 ± 0.075
2.472LeuHis: 2.472 ± 0.038
5.757LeuIle: 5.757 ± 0.07
4.175LeuLys: 4.175 ± 0.051
13.43LeuLeu: 13.43 ± 0.135
2.671LeuMet: 2.671 ± 0.044
3.503LeuAsn: 3.503 ± 0.052
6.176LeuPro: 6.176 ± 0.063
4.535LeuGln: 4.535 ± 0.052
7.455LeuArg: 7.455 ± 0.072
7.138LeuSer: 7.138 ± 0.065
5.79LeuThr: 5.79 ± 0.058
7.857LeuVal: 7.857 ± 0.08
1.354LeuTrp: 1.354 ± 0.031
2.544LeuTyr: 2.544 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
2.769MetAla: 2.769 ± 0.039
0.187MetCys: 0.187 ± 0.011
1.1MetAsp: 1.1 ± 0.025
1.083MetGlu: 1.083 ± 0.025
0.73MetPhe: 0.73 ± 0.019
1.722MetGly: 1.722 ± 0.031
0.541MetHis: 0.541 ± 0.018
1.204MetIle: 1.204 ± 0.025
0.984MetLys: 0.984 ± 0.022
2.873MetLeu: 2.873 ± 0.05
0.562MetMet: 0.562 ± 0.02
0.882MetAsn: 0.882 ± 0.025
1.445MetPro: 1.445 ± 0.026
1.154MetGln: 1.154 ± 0.027
1.678MetArg: 1.678 ± 0.033
1.758MetSer: 1.758 ± 0.03
1.581MetThr: 1.581 ± 0.035
1.66MetVal: 1.66 ± 0.032
0.191MetTrp: 0.191 ± 0.012
0.394MetTyr: 0.394 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.097AsnAla: 3.097 ± 0.041
0.301AsnCys: 0.301 ± 0.013
1.604AsnAsp: 1.604 ± 0.032
1.573AsnGlu: 1.573 ± 0.029
1.072AsnPhe: 1.072 ± 0.025
2.52AsnGly: 2.52 ± 0.041
0.687AsnHis: 0.687 ± 0.02
1.46AsnIle: 1.46 ± 0.033
0.969AsnLys: 0.969 ± 0.029
3.316AsnLeu: 3.316 ± 0.04
0.638AsnMet: 0.638 ± 0.019
0.947AsnAsn: 0.947 ± 0.028
1.947AsnPro: 1.947 ± 0.03
1.282AsnGln: 1.282 ± 0.027
2.007AsnArg: 2.007 ± 0.036
1.532AsnSer: 1.532 ± 0.032
1.422AsnThr: 1.422 ± 0.029
1.932AsnVal: 1.932 ± 0.036
0.47AsnTrp: 0.47 ± 0.016
0.788AsnTyr: 0.788 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
5.338ProAla: 5.338 ± 0.054
0.409ProCys: 0.409 ± 0.016
2.949ProAsp: 2.949 ± 0.039
3.488ProGlu: 3.488 ± 0.049
1.761ProPhe: 1.761 ± 0.03
3.848ProGly: 3.848 ± 0.047
1.056ProHis: 1.056 ± 0.027
2.165ProIle: 2.165 ± 0.035
1.585ProLys: 1.585 ± 0.031
5.427ProLeu: 5.427 ± 0.063
1.231ProMet: 1.231 ± 0.025
1.386ProAsn: 1.386 ± 0.03
2.001ProPro: 2.001 ± 0.043
2.138ProGln: 2.138 ± 0.033
2.664ProArg: 2.664 ± 0.045
2.762ProSer: 2.762 ± 0.039
2.368ProThr: 2.368 ± 0.037
3.711ProVal: 3.711 ± 0.047
0.734ProTrp: 0.734 ± 0.02
1.208ProTyr: 1.208 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
5.579GlnAla: 5.579 ± 0.064
0.349GlnCys: 0.349 ± 0.014
1.82GlnAsp: 1.82 ± 0.034
2.005GlnGlu: 2.005 ± 0.039
1.315GlnPhe: 1.315 ± 0.025
3.201GlnGly: 3.201 ± 0.042
1.136GlnHis: 1.136 ± 0.027
2.042GlnIle: 2.042 ± 0.035
1.224GlnLys: 1.224 ± 0.027
5.472GlnLeu: 5.472 ± 0.068
1.071GlnMet: 1.071 ± 0.023
1.13GlnAsn: 1.13 ± 0.025
2.458GlnPro: 2.458 ± 0.038
2.627GlnGln: 2.627 ± 0.051
3.61GlnArg: 3.61 ± 0.046
2.294GlnSer: 2.294 ± 0.04
2.024GlnThr: 2.024 ± 0.036
3.341GlnVal: 3.341 ± 0.045
0.637GlnTrp: 0.637 ± 0.015
0.944GlnTyr: 0.944 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
6.078ArgAla: 6.078 ± 0.065
0.7ArgCys: 0.7 ± 0.021
3.614ArgAsp: 3.614 ± 0.046
4.355ArgGlu: 4.355 ± 0.048
2.825ArgPhe: 2.825 ± 0.044
4.143ArgGly: 4.143 ± 0.047
1.729ArgHis: 1.729 ± 0.033
3.512ArgIle: 3.512 ± 0.047
2.21ArgLys: 2.21 ± 0.037
8.441ArgLeu: 8.441 ± 0.089
1.742ArgMet: 1.742 ± 0.031
2.032ArgAsn: 2.032 ± 0.032
2.841ArgPro: 2.841 ± 0.043
3.356ArgGln: 3.356 ± 0.048
4.648ArgArg: 4.648 ± 0.056
3.67ArgSer: 3.67 ± 0.048
2.898ArgThr: 2.898 ± 0.043
4.421ArgVal: 4.421 ± 0.054
1.134ArgTrp: 1.134 ± 0.025
2.199ArgTyr: 2.199 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
5.904SerAla: 5.904 ± 0.054
0.556SerCys: 0.556 ± 0.02
2.921SerAsp: 2.921 ± 0.043
3.244SerGlu: 3.244 ± 0.049
2.236SerPhe: 2.236 ± 0.032
5.222SerGly: 5.222 ± 0.054
1.306SerHis: 1.306 ± 0.028
2.898SerIle: 2.898 ± 0.045
1.848SerLys: 1.848 ± 0.033
6.865SerLeu: 6.865 ± 0.074
1.479SerMet: 1.479 ± 0.033
1.753SerAsn: 1.753 ± 0.037
2.67SerPro: 2.67 ± 0.04
2.396SerGln: 2.396 ± 0.038
3.8SerArg: 3.8 ± 0.047
3.481SerSer: 3.481 ± 0.05
2.791SerThr: 2.791 ± 0.043
4.061SerVal: 4.061 ± 0.048
0.844SerTrp: 0.844 ± 0.022
1.5SerTyr: 1.5 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
5.142ThrAla: 5.142 ± 0.058
0.459ThrCys: 0.459 ± 0.018
2.57ThrAsp: 2.57 ± 0.043
2.634ThrGlu: 2.634 ± 0.036
1.757ThrPhe: 1.757 ± 0.033
4.315ThrGly: 4.315 ± 0.047
1.103ThrHis: 1.103 ± 0.026
2.096ThrIle: 2.096 ± 0.035
1.241ThrLys: 1.241 ± 0.027
6.542ThrLeu: 6.542 ± 0.061
0.935ThrMet: 0.935 ± 0.025
1.272ThrAsn: 1.272 ± 0.028
3.017ThrPro: 3.017 ± 0.042
1.999ThrGln: 1.999 ± 0.034
3.053ThrArg: 3.053 ± 0.041
2.67ThrSer: 2.67 ± 0.038
2.423ThrThr: 2.423 ± 0.047
3.541ThrVal: 3.541 ± 0.047
0.674ThrTrp: 0.674 ± 0.02
1.182ThrTyr: 1.182 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
7.557ValAla: 7.557 ± 0.065
0.743ValCys: 0.743 ± 0.025
3.911ValAsp: 3.911 ± 0.052
4.442ValGlu: 4.442 ± 0.056
2.632ValPhe: 2.632 ± 0.04
5.196ValGly: 5.196 ± 0.061
1.434ValHis: 1.434 ± 0.029
3.899ValIle: 3.899 ± 0.047
2.303ValLys: 2.303 ± 0.043
8.152ValLeu: 8.152 ± 0.076
1.837ValMet: 1.837 ± 0.033
2.131ValAsn: 2.131 ± 0.036
3.312ValPro: 3.312 ± 0.049
2.58ValGln: 2.58 ± 0.037
4.34ValArg: 4.34 ± 0.053
4.275ValSer: 4.275 ± 0.049
3.691ValThr: 3.691 ± 0.053
5.446ValVal: 5.446 ± 0.066
0.899ValTrp: 0.899 ± 0.023
1.56ValTyr: 1.56 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.199TrpAla: 1.199 ± 0.026
0.167TrpCys: 0.167 ± 0.01
0.631TrpAsp: 0.631 ± 0.019
0.604TrpGlu: 0.604 ± 0.019
0.546TrpPhe: 0.546 ± 0.019
0.895TrpGly: 0.895 ± 0.023
0.407TrpHis: 0.407 ± 0.015
0.664TrpIle: 0.664 ± 0.021
0.448TrpLys: 0.448 ± 0.015
2.269TrpLeu: 2.269 ± 0.041
0.424TrpMet: 0.424 ± 0.015
0.477TrpAsn: 0.477 ± 0.018
0.678TrpPro: 0.678 ± 0.02
0.822TrpGln: 0.822 ± 0.021
1.13TrpArg: 1.13 ± 0.027
0.815TrpSer: 0.815 ± 0.019
0.64TrpThr: 0.64 ± 0.022
0.99TrpVal: 0.99 ± 0.025
0.238TrpTrp: 0.238 ± 0.012
0.348TrpTyr: 0.348 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.456TyrAla: 2.456 ± 0.033
0.316TyrCys: 0.316 ± 0.015
1.323TyrAsp: 1.323 ± 0.026
1.32TyrGlu: 1.32 ± 0.028
1.025TyrPhe: 1.025 ± 0.023
2.088TyrGly: 2.088 ± 0.029
0.589TyrHis: 0.589 ± 0.017
1.02TyrIle: 1.02 ± 0.027
0.799TyrLys: 0.799 ± 0.024
3.073TyrLeu: 3.073 ± 0.038
0.459TyrMet: 0.459 ± 0.018
0.713TyrAsn: 0.713 ± 0.021
1.278TyrPro: 1.278 ± 0.027
1.261TyrGln: 1.261 ± 0.029
2.047TyrArg: 2.047 ± 0.037
1.516TyrSer: 1.516 ± 0.032
1.163TyrThr: 1.163 ± 0.027
1.647TyrVal: 1.647 ± 0.029
0.438TyrTrp: 0.438 ± 0.017
0.725TyrTyr: 0.725 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6978 proteins (1805302 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski