Lysinibacillus yapensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lysinibacillus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3570 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A396SAU4|A0A396SAU4_9BACI Aminopeptidase P family protein OS=Lysinibacillus yapensis OX=2304605 GN=D1B33_06275 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.4KK3 pKa = 10.38LGIIGAIVVVLFVGIIILTNMSNEE27 pKa = 4.21DD28 pKa = 3.3KK29 pKa = 10.86LANNPYY35 pKa = 8.92GTDD38 pKa = 3.77DD39 pKa = 4.24LRR41 pKa = 11.84QSTIDD46 pKa = 4.32LLDD49 pKa = 4.19DD50 pKa = 3.9EE51 pKa = 5.58NYY53 pKa = 10.62QNIILPEE60 pKa = 3.98ALEE63 pKa = 4.11EE64 pKa = 4.5KK65 pKa = 10.16IATGEE70 pKa = 3.95PVVAYY75 pKa = 7.65MFSPEE80 pKa = 4.43CPHH83 pKa = 5.99CQKK86 pKa = 8.14MTPALMPIADD96 pKa = 3.9EE97 pKa = 4.46VGVQVDD103 pKa = 3.32QLNILEE109 pKa = 4.54YY110 pKa = 9.39EE111 pKa = 4.39QGWTDD116 pKa = 3.64YY117 pKa = 11.03NIEE120 pKa = 4.09ATPTLIYY127 pKa = 10.76FNDD130 pKa = 3.75GKK132 pKa = 10.56EE133 pKa = 4.01VNRR136 pKa = 11.84IVGDD140 pKa = 3.51YY141 pKa = 10.99SQDD144 pKa = 2.92TDD146 pKa = 3.99VIYY149 pKa = 10.99DD150 pKa = 3.27FLGQVKK156 pKa = 10.14

Molecular weight: 17.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A396S6P5|A0A396S6P5_9BACI YycH domain-containing protein OS=Lysinibacillus yapensis OX=2304605 GN=D1B33_10865 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.48NGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3570

0

3570

1043971

27

1441

292.4

32.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.326 ± 0.04

0.677 ± 0.012

4.866 ± 0.032

7.811 ± 0.057

4.625 ± 0.04

6.78 ± 0.037

2.015 ± 0.02

8.057 ± 0.04

6.639 ± 0.039

9.788 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.02

4.596 ± 0.034

3.581 ± 0.026

3.797 ± 0.03

3.873 ± 0.032

5.995 ± 0.032

5.375 ± 0.028

7.048 ± 0.031

0.945 ± 0.015

3.484 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski