Paracandidimonas soli
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3626 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R3UQX3|A0A4R3UQX3_9BURK Nitric oxide reductase NorD protein OS=Paracandidimonas soli OX=1917182 GN=EV686_11284 PE=4 SV=1
MM1 pKa = 7.65 EE2 pKa = 5.85 LPDD5 pKa = 4.38 EE6 pKa = 4.67 IYY8 pKa = 10.79 EE9 pKa = 4.12 QVEE12 pKa = 4.02 QLSEE16 pKa = 3.97 QGNDD20 pKa = 3.1 LCEE23 pKa = 4.39 EE24 pKa = 4.47 GDD26 pKa = 3.73 FTGAIAVWQSALALLPPPRR45 pKa = 11.84 QQWEE49 pKa = 3.97 ASVWLHH55 pKa = 6.19 ASIGDD60 pKa = 3.57 AYY62 pKa = 10.09 YY63 pKa = 10.61 QEE65 pKa = 6.06 DD66 pKa = 3.56 EE67 pKa = 4.28 WQDD70 pKa = 2.93 AKK72 pKa = 11.39 AAFFDD77 pKa = 5.08 ALNCPDD83 pKa = 3.6 AQVNPFVHH91 pKa = 6.12 YY92 pKa = 10.07 MLGKK96 pKa = 8.39 TLLRR100 pKa = 11.84 LDD102 pKa = 4.42 DD103 pKa = 4.01 EE104 pKa = 4.95 EE105 pKa = 5.33 GAVDD109 pKa = 3.78 HH110 pKa = 6.9 LLRR113 pKa = 11.84 AYY115 pKa = 9.86 MLDD118 pKa = 3.4 GVDD121 pKa = 4.77 IFDD124 pKa = 4.37 ADD126 pKa = 3.89 EE127 pKa = 4.57 EE128 pKa = 4.36 EE129 pKa = 5.42 GPDD132 pKa = 5.25 ALALLQNKK140 pKa = 9.39 GLVDD144 pKa = 3.74 EE145 pKa = 4.93 LL146 pKa = 4.18
Molecular weight: 16.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.592
Rodwell 3.503
Grimsley 3.376
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 3.986
Thurlkill 3.516
EMBOSS 3.605
Sillero 3.795
Patrickios 1.837
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A4V2VSK3|A0A4V2VSK3_9BURK 3-dehydroquinate synthase OS=Paracandidimonas soli OX=1917182 GN=aroB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 8.99 TRR25 pKa = 11.84 AGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3626
0
3626
1167027
25
2121
321.8
35.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.796 ± 0.053
0.938 ± 0.014
5.379 ± 0.033
5.45 ± 0.04
3.437 ± 0.027
8.263 ± 0.041
2.276 ± 0.019
5.07 ± 0.028
3.118 ± 0.034
10.82 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.645 ± 0.02
2.721 ± 0.023
5.129 ± 0.027
4.14 ± 0.03
6.953 ± 0.039
5.932 ± 0.026
4.939 ± 0.023
7.192 ± 0.035
1.413 ± 0.017
2.386 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here