Shimazuella sp. KC615

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Shimazuella; unclassified Shimazuella

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3870 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I4VQJ4|A0A6I4VQJ4_9BACL Alpha/beta fold hydrolase OS=Shimazuella sp. KC615 OX=2690964 GN=GSM42_02000 PE=4 SV=1
MM1 pKa = 7.2RR2 pKa = 11.84TWVDD6 pKa = 2.81KK7 pKa = 9.29DD8 pKa = 3.37TCIACGACGATAPDD22 pKa = 3.76VYY24 pKa = 10.95DD25 pKa = 3.8YY26 pKa = 11.63DD27 pKa = 3.82EE28 pKa = 5.96DD29 pKa = 6.09GIAFVTLDD37 pKa = 4.59DD38 pKa = 3.97NTGTADD44 pKa = 3.7VPEE47 pKa = 5.0DD48 pKa = 3.37LHH50 pKa = 9.38DD51 pKa = 4.52DD52 pKa = 3.56VRR54 pKa = 11.84DD55 pKa = 3.85AQEE58 pKa = 4.25GCPTDD63 pKa = 3.78SIKK66 pKa = 10.82VEE68 pKa = 4.13EE69 pKa = 4.22

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I4VRX9|A0A6I4VRX9_9BACL NAD(P)-binding domain-containing protein OS=Shimazuella sp. KC615 OX=2690964 GN=GSM42_08555 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.23QPSNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.7NVHH16 pKa = 5.09GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.13VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3870

0

3870

1015819

26

6194

262.5

29.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.773 ± 0.043

0.848 ± 0.01

4.864 ± 0.03

6.777 ± 0.047

4.433 ± 0.038

6.567 ± 0.039

2.36 ± 0.02

7.744 ± 0.039

6.66 ± 0.04

10.154 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.019

4.018 ± 0.03

3.842 ± 0.025

4.364 ± 0.033

4.515 ± 0.032

6.243 ± 0.028

5.533 ± 0.025

6.939 ± 0.039

1.253 ± 0.019

3.454 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski