Amino acid dipepetide frequency for Shimazuella sp. KC615

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.008AlaAla: 5.008 ± 0.104	0.632AlaCys: 0.632 ± 0.027	3.31AlaAsp: 3.31 ± 0.057	4.289AlaGlu: 4.289 ± 0.069	3.079AlaPhe: 3.079 ± 0.067	4.954AlaGly: 4.954 ± 0.075	1.417AlaHis: 1.417 ± 0.045	5.75AlaIle: 5.75 ± 0.081	4.881AlaLys: 4.881 ± 0.076	6.841AlaLeu: 6.841 ± 0.09	1.884AlaMet: 1.884 ± 0.044	2.783AlaAsn: 2.783 ± 0.047	1.979AlaPro: 1.979 ± 0.045	2.307AlaGln: 2.307 ± 0.056	2.801AlaArg: 2.801 ± 0.059	3.999AlaSer: 3.999 ± 0.073	3.798AlaThr: 3.798 ± 0.086	4.941AlaVal: 4.941 ± 0.078	0.764AlaTrp: 0.764 ± 0.029	2.317AlaTyr: 2.317 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.43CysAla: 0.43 ± 0.019	0.125CysCys: 0.125 ± 0.012	0.416CysAsp: 0.416 ± 0.021	0.479CysGlu: 0.479 ± 0.022	0.459CysPhe: 0.459 ± 0.023	0.71CysGly: 0.71 ± 0.032	0.227CysHis: 0.227 ± 0.017	0.638CysIle: 0.638 ± 0.029	0.485CysLys: 0.485 ± 0.025	0.822CysLeu: 0.822 ± 0.031	0.223CysMet: 0.223 ± 0.014	0.322CysAsn: 0.322 ± 0.017	0.412CysPro: 0.412 ± 0.024	0.283CysGln: 0.283 ± 0.019	0.375CysArg: 0.375 ± 0.02	0.599CysSer: 0.599 ± 0.024	0.458CysThr: 0.458 ± 0.022	0.548CysVal: 0.548 ± 0.023	0.111CysTrp: 0.111 ± 0.012	0.352CysTyr: 0.352 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.127AspAla: 3.127 ± 0.053	0.411AspCys: 0.411 ± 0.02	2.237AspAsp: 2.237 ± 0.055	3.747AspGlu: 3.747 ± 0.063	2.142AspPhe: 2.142 ± 0.049	3.194AspGly: 3.194 ± 0.059	1.272AspHis: 1.272 ± 0.032	3.699AspIle: 3.699 ± 0.07	2.922AspLys: 2.922 ± 0.054	5.043AspLeu: 5.043 ± 0.071	1.212AspMet: 1.212 ± 0.039	1.73AspAsn: 1.73 ± 0.049	2.061AspPro: 2.061 ± 0.048	2.439AspGln: 2.439 ± 0.058	2.604AspArg: 2.604 ± 0.059	2.527AspSer: 2.527 ± 0.05	2.338AspThr: 2.338 ± 0.052	3.379AspVal: 3.379 ± 0.06	0.755AspTrp: 0.755 ± 0.031	1.802AspTyr: 1.802 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	4.814GluAla: 4.814 ± 0.072	0.407GluCys: 0.407 ± 0.019	3.185GluAsp: 3.185 ± 0.057	5.964GluGlu: 5.964 ± 0.11	2.068GluPhe: 2.068 ± 0.051	3.693GluGly: 3.693 ± 0.065	1.453GluHis: 1.453 ± 0.04	5.183GluIle: 5.183 ± 0.081	5.983GluLys: 5.983 ± 0.093	6.714GluLeu: 6.714 ± 0.089	1.906GluMet: 1.906 ± 0.054	3.132GluAsn: 3.132 ± 0.061	1.804GluPro: 1.804 ± 0.041	3.738GluGln: 3.738 ± 0.079	3.291GluArg: 3.291 ± 0.064	3.308GluSer: 3.308 ± 0.059	3.395GluThr: 3.395 ± 0.066	4.813GluVal: 4.813 ± 0.077	0.853GluTrp: 0.853 ± 0.033	2.07GluTyr: 2.07 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	3.199PheAla: 3.199 ± 0.064	0.447PheCys: 0.447 ± 0.023	2.264PheAsp: 2.264 ± 0.048	2.381PheGlu: 2.381 ± 0.053	2.368PhePhe: 2.368 ± 0.058	3.139PheGly: 3.139 ± 0.076	1.31PheHis: 1.31 ± 0.039	3.405PheIle: 3.405 ± 0.066	1.616PheLys: 1.616 ± 0.044	4.798PheLeu: 4.798 ± 0.09	0.998PheMet: 0.998 ± 0.034	1.198PheAsn: 1.198 ± 0.031	1.937PhePro: 1.937 ± 0.048	2.204PheGln: 2.204 ± 0.05	1.885PheArg: 1.885 ± 0.045	3.242PheSer: 3.242 ± 0.059	2.601PheThr: 2.601 ± 0.049	3.2PheVal: 3.2 ± 0.062	0.534PheTrp: 0.534 ± 0.025	1.6PheTyr: 1.6 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	4.378GlyAla: 4.378 ± 0.103	0.689GlyCys: 0.689 ± 0.031	2.959GlyAsp: 2.959 ± 0.063	4.062GlyGlu: 4.062 ± 0.075	3.157GlyPhe: 3.157 ± 0.065	4.365GlyGly: 4.365 ± 0.09	1.393GlyHis: 1.393 ± 0.038	5.723GlyIle: 5.723 ± 0.08	4.98GlyLys: 4.98 ± 0.079	6.263GlyLeu: 6.263 ± 0.096	1.951GlyMet: 1.951 ± 0.046	2.546GlyAsn: 2.546 ± 0.052	1.751GlyPro: 1.751 ± 0.043	2.236GlyGln: 2.236 ± 0.055	2.882GlyArg: 2.882 ± 0.063	3.885GlySer: 3.885 ± 0.074	3.856GlyThr: 3.856 ± 0.068	5.089GlyVal: 5.089 ± 0.068	0.966GlyTrp: 0.966 ± 0.033	2.536GlyTyr: 2.536 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	1.503HisAla: 1.503 ± 0.039	0.196HisCys: 0.196 ± 0.014	1.039HisAsp: 1.039 ± 0.028	1.34HisGlu: 1.34 ± 0.035	1.197HisPhe: 1.197 ± 0.038	1.435HisGly: 1.435 ± 0.04	0.89HisHis: 0.89 ± 0.035	1.775HisIle: 1.775 ± 0.04	1.113HisLys: 1.113 ± 0.036	2.764HisLeu: 2.764 ± 0.056	0.569HisMet: 0.569 ± 0.024	0.719HisAsn: 0.719 ± 0.027	1.331HisPro: 1.331 ± 0.039	1.177HisGln: 1.177 ± 0.039	1.128HisArg: 1.128 ± 0.035	1.336HisSer: 1.336 ± 0.037	1.303HisThr: 1.303 ± 0.034	1.499HisVal: 1.499 ± 0.041	0.298HisTrp: 0.298 ± 0.017	0.987HisTyr: 0.987 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	6.014IleAla: 6.014 ± 0.081	0.829IleCys: 0.829 ± 0.03	4.091IleAsp: 4.091 ± 0.072	4.87IleGlu: 4.87 ± 0.08	3.212IlePhe: 3.212 ± 0.065	5.98IleGly: 5.98 ± 0.095	2.045IleHis: 2.045 ± 0.046	5.209IleIle: 5.209 ± 0.081	4.014IleLys: 4.014 ± 0.064	7.499IleLeu: 7.499 ± 0.104	1.613IleMet: 1.613 ± 0.04	2.701IleAsn: 2.701 ± 0.046	3.693IlePro: 3.693 ± 0.056	3.479IleGln: 3.479 ± 0.07	3.657IleArg: 3.657 ± 0.063	5.396IleSer: 5.396 ± 0.073	4.501IleThr: 4.501 ± 0.069	5.165IleVal: 5.165 ± 0.078	0.917IleTrp: 0.917 ± 0.037	2.555IleTyr: 2.555 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	4.285LysAla: 4.285 ± 0.073	0.393LysCys: 0.393 ± 0.02	3.183LysAsp: 3.183 ± 0.058	5.839LysGlu: 5.839 ± 0.095	1.786LysPhe: 1.786 ± 0.043	3.971LysGly: 3.971 ± 0.062	1.37LysHis: 1.37 ± 0.038	4.721LysIle: 4.721 ± 0.082	5.861LysLys: 5.861 ± 0.097	5.95LysLeu: 5.95 ± 0.09	2.053LysMet: 2.053 ± 0.049	3.218LysAsn: 3.218 ± 0.058	2.322LysPro: 2.322 ± 0.053	3.711LysGln: 3.711 ± 0.063	3.445LysArg: 3.445 ± 0.062	3.542LysSer: 3.542 ± 0.058	3.463LysThr: 3.463 ± 0.062	4.405LysVal: 4.405 ± 0.071	1.007LysTrp: 1.007 ± 0.031	2.079LysTyr: 2.079 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	7.521LeuAla: 7.521 ± 0.094	0.905LeuCys: 0.905 ± 0.032	5.001LeuAsp: 5.001 ± 0.087	6.685LeuGlu: 6.685 ± 0.092	5.098LeuPhe: 5.098 ± 0.082	6.621LeuGly: 6.621 ± 0.092	2.46LeuHis: 2.46 ± 0.048	7.212LeuIle: 7.212 ± 0.107	5.728LeuLys: 5.728 ± 0.082	10.838LeuLeu: 10.838 ± 0.126	2.503LeuMet: 2.503 ± 0.054	3.503LeuAsn: 3.503 ± 0.066	4.388LeuPro: 4.388 ± 0.076	4.516LeuGln: 4.516 ± 0.082	4.37LeuArg: 4.37 ± 0.074	7.389LeuSer: 7.389 ± 0.094	5.789LeuThr: 5.789 ± 0.071	6.804LeuVal: 6.804 ± 0.085	1.04LeuTrp: 1.04 ± 0.038	3.171LeuTyr: 3.171 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	1.686MetAla: 1.686 ± 0.048	0.146MetCys: 0.146 ± 0.012	1.454MetAsp: 1.454 ± 0.038	2.114MetGlu: 2.114 ± 0.05	0.98MetPhe: 0.98 ± 0.037	1.751MetGly: 1.751 ± 0.039	0.491MetHis: 0.491 ± 0.021	2.153MetIle: 2.153 ± 0.042	2.139MetLys: 2.139 ± 0.048	2.425MetLeu: 2.425 ± 0.05	0.898MetMet: 0.898 ± 0.033	1.339MetAsn: 1.339 ± 0.041	0.896MetPro: 0.896 ± 0.031	1.007MetGln: 1.007 ± 0.032	1.202MetArg: 1.202 ± 0.038	1.689MetSer: 1.689 ± 0.05	1.324MetThr: 1.324 ± 0.031	1.915MetVal: 1.915 ± 0.044	0.239MetTrp: 0.239 ± 0.015	0.74MetTyr: 0.74 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.429AsnAla: 2.429 ± 0.054	0.296AsnCys: 0.296 ± 0.017	1.845AsnAsp: 1.845 ± 0.044	2.648AsnGlu: 2.648 ± 0.06	1.447AsnPhe: 1.447 ± 0.036	2.833AsnGly: 2.833 ± 0.059	1.07AsnHis: 1.07 ± 0.036	2.912AsnIle: 2.912 ± 0.059	2.782AsnLys: 2.782 ± 0.061	3.871AsnLeu: 3.871 ± 0.065	1.054AsnMet: 1.054 ± 0.032	1.739AsnAsn: 1.739 ± 0.056	2.19AsnPro: 2.19 ± 0.05	2.242AsnGln: 2.242 ± 0.049	2.23AsnArg: 2.23 ± 0.048	2.146AsnSer: 2.146 ± 0.052	2.048AsnThr: 2.048 ± 0.046	2.449AsnVal: 2.449 ± 0.049	0.663AsnTrp: 0.663 ± 0.028	1.289AsnTyr: 1.289 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	2.405ProAla: 2.405 ± 0.051	0.256ProCys: 0.256 ± 0.017	2.113ProAsp: 2.113 ± 0.046	2.759ProGlu: 2.759 ± 0.051	2.0ProPhe: 2.0 ± 0.051	2.055ProGly: 2.055 ± 0.048	0.917ProHis: 0.917 ± 0.03	3.297ProIle: 3.297 ± 0.068	2.565ProLys: 2.565 ± 0.046	3.778ProLeu: 3.778 ± 0.068	0.878ProMet: 0.878 ± 0.029	1.825ProAsn: 1.825 ± 0.044	1.178ProPro: 1.178 ± 0.037	1.235ProGln: 1.235 ± 0.035	1.238ProArg: 1.238 ± 0.035	2.705ProSer: 2.705 ± 0.052	2.35ProThr: 2.35 ± 0.061	2.942ProVal: 2.942 ± 0.054	0.468ProTrp: 0.468 ± 0.021	1.453ProTyr: 1.453 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	3.126GlnAla: 3.126 ± 0.056	0.241GlnCys: 0.241 ± 0.017	1.811GlnAsp: 1.811 ± 0.044	3.105GlnGlu: 3.105 ± 0.074	1.777GlnPhe: 1.777 ± 0.036	2.281GlnGly: 2.281 ± 0.043	0.943GlnHis: 0.943 ± 0.035	3.388GlnIle: 3.388 ± 0.061	3.44GlnLys: 3.44 ± 0.054	4.916GlnLeu: 4.916 ± 0.101	1.349GlnMet: 1.349 ± 0.036	1.821GlnAsn: 1.821 ± 0.041	1.327GlnPro: 1.327 ± 0.039	2.279GlnGln: 2.279 ± 0.064	1.731GlnArg: 1.731 ± 0.045	2.497GlnSer: 2.497 ± 0.053	2.471GlnThr: 2.471 ± 0.05	3.202GlnVal: 3.202 ± 0.065	0.471GlnTrp: 0.471 ± 0.022	1.467GlnTyr: 1.467 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.606ArgAla: 2.606 ± 0.056	0.368ArgCys: 0.368 ± 0.018	2.243ArgAsp: 2.243 ± 0.046	3.277ArgGlu: 3.277 ± 0.06	2.239ArgPhe: 2.239 ± 0.046	2.524ArgGly: 2.524 ± 0.056	0.913ArgHis: 0.913 ± 0.03	3.429ArgIle: 3.429 ± 0.055	3.573ArgLys: 3.573 ± 0.056	4.686ArgLeu: 4.686 ± 0.076	1.297ArgMet: 1.297 ± 0.035	2.064ArgAsn: 2.064 ± 0.056	1.496ArgPro: 1.496 ± 0.042	1.734ArgGln: 1.734 ± 0.046	2.329ArgArg: 2.329 ± 0.049	2.661ArgSer: 2.661 ± 0.053	2.286ArgThr: 2.286 ± 0.055	3.049ArgVal: 3.049 ± 0.057	0.548ArgTrp: 0.548 ± 0.022	1.827ArgTyr: 1.827 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	3.765SerAla: 3.765 ± 0.063	0.537SerCys: 0.537 ± 0.027	2.895SerAsp: 2.895 ± 0.062	3.395SerGlu: 3.395 ± 0.057	3.399SerPhe: 3.399 ± 0.065	4.221SerGly: 4.221 ± 0.069	1.498SerHis: 1.498 ± 0.04	5.425SerIle: 5.425 ± 0.08	3.951SerLys: 3.951 ± 0.059	6.777SerLeu: 6.777 ± 0.091	1.801SerMet: 1.801 ± 0.041	2.545SerAsn: 2.545 ± 0.048	2.462SerPro: 2.462 ± 0.053	2.238SerGln: 2.238 ± 0.053	2.667SerArg: 2.667 ± 0.053	4.368SerSer: 4.368 ± 0.08	3.403SerThr: 3.403 ± 0.057	3.935SerVal: 3.935 ± 0.066	0.771SerTrp: 0.771 ± 0.026	2.373SerTyr: 2.373 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	3.89ThrAla: 3.89 ± 0.065	0.487ThrCys: 0.487 ± 0.023	2.635ThrAsp: 2.635 ± 0.057	3.279ThrGlu: 3.279 ± 0.057	2.574ThrPhe: 2.574 ± 0.048	4.231ThrGly: 4.231 ± 0.099	1.151ThrHis: 1.151 ± 0.031	4.547ThrIle: 4.547 ± 0.07	3.6ThrLys: 3.6 ± 0.064	5.405ThrLeu: 5.405 ± 0.076	1.372ThrMet: 1.372 ± 0.038	2.445ThrAsn: 2.445 ± 0.048	2.512ThrPro: 2.512 ± 0.051	1.783ThrGln: 1.783 ± 0.046	2.041ThrArg: 2.041 ± 0.048	3.58ThrSer: 3.58 ± 0.053	3.314ThrThr: 3.314 ± 0.059	3.781ThrVal: 3.781 ± 0.063	0.664ThrTrp: 0.664 ± 0.029	2.039ThrTyr: 2.039 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	4.802ValAla: 4.802 ± 0.08	0.69ValCys: 0.69 ± 0.027	3.619ValAsp: 3.619 ± 0.069	4.612ValGlu: 4.612 ± 0.07	3.011ValPhe: 3.011 ± 0.055	4.607ValGly: 4.607 ± 0.073	1.522ValHis: 1.522 ± 0.035	5.547ValIle: 5.547 ± 0.089	4.308ValLys: 4.308 ± 0.069	6.872ValLeu: 6.872 ± 0.091	1.76ValMet: 1.76 ± 0.042	2.657ValAsn: 2.657 ± 0.053	2.813ValPro: 2.813 ± 0.059	2.589ValGln: 2.589 ± 0.046	2.845ValArg: 2.845 ± 0.061	4.763ValSer: 4.763 ± 0.069	4.158ValThr: 4.158 ± 0.068	5.264ValVal: 5.264 ± 0.076	0.778ValTrp: 0.778 ± 0.027	2.17ValTyr: 2.17 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.686TrpAla: 0.686 ± 0.029	0.102TrpCys: 0.102 ± 0.011	0.607TrpAsp: 0.607 ± 0.024	0.852TrpGlu: 0.852 ± 0.036	0.648TrpPhe: 0.648 ± 0.029	0.771TrpGly: 0.771 ± 0.029	0.227TrpHis: 0.227 ± 0.016	1.131TrpIle: 1.131 ± 0.037	0.89TrpLys: 0.89 ± 0.035	1.402TrpLeu: 1.402 ± 0.039	0.443TrpMet: 0.443 ± 0.022	0.685TrpAsn: 0.685 ± 0.029	0.299TrpPro: 0.299 ± 0.02	0.44TrpGln: 0.44 ± 0.023	0.53TrpArg: 0.53 ± 0.025	0.79TrpSer: 0.79 ± 0.031	0.629TrpThr: 0.629 ± 0.027	0.782TrpVal: 0.782 ± 0.034	0.206TrpTrp: 0.206 ± 0.015	0.411TrpTyr: 0.411 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.058TyrAla: 2.058 ± 0.042	0.319TyrCys: 0.319 ± 0.02	1.735TyrAsp: 1.735 ± 0.039	2.076TyrGlu: 2.076 ± 0.048	1.686TyrPhe: 1.686 ± 0.041	2.33TyrGly: 2.33 ± 0.05	1.032TyrHis: 1.032 ± 0.031	2.296TyrIle: 2.296 ± 0.054	1.77TyrLys: 1.77 ± 0.044	3.878TyrLeu: 3.878 ± 0.071	0.821TyrMet: 0.821 ± 0.032	1.21TyrAsn: 1.21 ± 0.037	1.565TyrPro: 1.565 ± 0.04	2.006TyrGln: 2.006 ± 0.044	1.899TyrArg: 1.899 ± 0.048	2.006TyrSer: 2.006 ± 0.045	1.847TyrThr: 1.847 ± 0.045	2.224TyrVal: 2.224 ± 0.05	0.48TyrTrp: 0.48 ± 0.023	1.301TyrTyr: 1.301 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3870 proteins (1015819 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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