Fonticula alba (Slime mold)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Rotosphaerida; Fonticulaceae; Fonticula

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A058ZDV0|A0A058ZDV0_FONAL VPS11_C domain-containing protein OS=Fonticula alba OX=691883 GN=H696_01059 PE=3 SV=1
MM1 pKa = 7.56ASSLFRR7 pKa = 11.84AAPLMADD14 pKa = 4.45TFTQAQPPVAPGTEE28 pKa = 4.19QSAMPAQSAPPAHH41 pKa = 6.7PTSMPQTPGTPPPALVDD58 pKa = 3.55PFFSGRR64 pKa = 11.84GPTGQEE70 pKa = 3.14ADD72 pKa = 3.83LLGEE76 pKa = 4.02GLVTHH81 pKa = 6.59EE82 pKa = 4.58AHH84 pKa = 7.65DD85 pKa = 4.8DD86 pKa = 3.65GAGAFDD92 pKa = 5.63DD93 pKa = 4.3GRR95 pKa = 11.84LDD97 pKa = 4.76ARR99 pKa = 11.84PEE101 pKa = 3.9DD102 pKa = 4.2LGADD106 pKa = 4.02GDD108 pKa = 5.55DD109 pKa = 6.03DD110 pKa = 7.02DD111 pKa = 7.76DD112 pKa = 7.72DD113 pKa = 7.61DD114 pKa = 7.68DD115 pKa = 7.64DD116 pKa = 7.68DD117 pKa = 7.61DD118 pKa = 7.65DD119 pKa = 7.0DD120 pKa = 5.71DD121 pKa = 5.74LAMTPRR127 pKa = 11.84VAAQFYY133 pKa = 10.12LPQIYY138 pKa = 10.47GLFASLFATFFLVIWWAPTIFLSVPFNYY166 pKa = 9.9TGVVLSTGTNTLSTGSQLLVAVASAAILLVIILVNTVILLALFYY210 pKa = 10.35YY211 pKa = 10.49GYY213 pKa = 10.51IRR215 pKa = 11.84VIYY218 pKa = 9.46GWLIVATGLILSFIGGIVFYY238 pKa = 10.8RR239 pKa = 11.84LLEE242 pKa = 4.37TYY244 pKa = 9.9NLPVDD249 pKa = 3.47WVTFCFLLFNFSVVGLLAVFGKK271 pKa = 8.77LTLRR275 pKa = 11.84MQQVYY280 pKa = 9.79LVIVSALVATNLSGLPSWTAWAVLIVVSIYY310 pKa = 11.18DD311 pKa = 4.26LFAVLCPQGPLNLLINLSQTRR332 pKa = 11.84QQSIPALLYY341 pKa = 8.77SAGMARR347 pKa = 11.84LASSNDD353 pKa = 3.1DD354 pKa = 3.66SVPILAFTAGTSDD367 pKa = 4.67DD368 pKa = 4.7SGDD371 pKa = 3.99EE372 pKa = 4.05QPEE375 pKa = 4.13DD376 pKa = 4.07ADD378 pKa = 4.14TQVGQALLSSGHH390 pKa = 6.62LEE392 pKa = 4.05SVLLGEE398 pKa = 5.12AMPSAASDD406 pKa = 3.84LDD408 pKa = 4.05SAAVPAIDD416 pKa = 3.9STTGNPILDD425 pKa = 4.26AEE427 pKa = 4.58TGPKK431 pKa = 9.96DD432 pKa = 4.17VSDD435 pKa = 4.06EE436 pKa = 4.1QDD438 pKa = 4.88DD439 pKa = 4.45GDD441 pKa = 5.8DD442 pKa = 5.33DD443 pKa = 5.35DD444 pKa = 7.07GEE446 pKa = 6.33DD447 pKa = 3.78GDD449 pKa = 4.54EE450 pKa = 4.35PPGRR454 pKa = 11.84GPVQLGLGDD463 pKa = 4.11YY464 pKa = 10.34IFYY467 pKa = 10.74SLLVARR473 pKa = 11.84GALTSDD479 pKa = 3.65GSDD482 pKa = 3.08GGASAAIAVISCVLSTLVGLGLTLSLLALFRR513 pKa = 11.84KK514 pKa = 8.81PLPALPISIGLGILAYY530 pKa = 10.38VFSAFLSTPMVVEE543 pKa = 4.12MTRR546 pKa = 11.84QVVFVV551 pKa = 3.87

Molecular weight: 58.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A058Z4E6|A0A058Z4E6_FONAL Uncharacterized protein OS=Fonticula alba OX=691883 GN=H696_04391 PE=4 SV=1
MM1 pKa = 7.53RR2 pKa = 11.84SAWSSRR8 pKa = 11.84RR9 pKa = 11.84SASRR13 pKa = 11.84EE14 pKa = 3.57GSFIAPAAGSAPAGPPPMRR33 pKa = 11.84LRR35 pKa = 11.84RR36 pKa = 11.84IRR38 pKa = 11.84PPRR41 pKa = 11.84PRR43 pKa = 11.84PRR45 pKa = 11.84LLRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84PLAPAIPLGPSRR66 pKa = 4.73

Molecular weight: 7.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5901

343

6244

4258807

42

6752

682.1

71.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.272 ± 0.054

1.723 ± 0.036

5.239 ± 0.024

4.49 ± 0.027

3.144 ± 0.018

8.784 ± 0.049

2.683 ± 0.019

3.126 ± 0.024

2.001 ± 0.021

9.905 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.905 ± 0.011

2.068 ± 0.017

8.675 ± 0.051

3.668 ± 0.027

6.665 ± 0.026

9.101 ± 0.048

5.177 ± 0.017

5.723 ± 0.022

0.937 ± 0.008

1.712 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski