Erwinia phage vB_EamM_Caitlin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Machinavirus; unclassified Machinavirus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2IBC3|A0A1B2IBC3_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Caitlin OX=1883379 GN=CAITLIN_275 PE=4 SV=1
MM1 pKa = 7.14LTIEE5 pKa = 4.2VPEE8 pKa = 3.99LRR10 pKa = 11.84RR11 pKa = 11.84YY12 pKa = 9.03NQDD15 pKa = 2.61FFRR18 pKa = 11.84QLFDD22 pKa = 3.68KK23 pKa = 10.43LWPGFQIGHH32 pKa = 6.9LGEE35 pKa = 4.85GFHH38 pKa = 7.12DD39 pKa = 3.38RR40 pKa = 11.84HH41 pKa = 7.86IIDD44 pKa = 3.48VFFVDD49 pKa = 4.89SVSLLMDD56 pKa = 4.2TFSHH60 pKa = 6.44SVDD63 pKa = 3.38EE64 pKa = 4.63VVQAANLRR72 pKa = 11.84RR73 pKa = 11.84PFDD76 pKa = 3.97LDD78 pKa = 3.64YY79 pKa = 11.62VIFDD83 pKa = 4.12HH84 pKa = 6.99NDD86 pKa = 3.13YY87 pKa = 10.37MDD89 pKa = 3.84TYY91 pKa = 10.94SFEE94 pKa = 5.18DD95 pKa = 4.1PEE97 pKa = 4.56LPCDD101 pKa = 3.62KK102 pKa = 10.59RR103 pKa = 11.84EE104 pKa = 4.09HH105 pKa = 6.53FEE107 pKa = 3.72GLMFEE112 pKa = 4.55FFNYY116 pKa = 9.96LVEE119 pKa = 4.22TLQEE123 pKa = 4.23EE124 pKa = 4.8EE125 pKa = 4.23FMAAITADD133 pKa = 3.67LTKK136 pKa = 9.89VWNSIAPIVGTHH148 pKa = 6.7GIVGFWYY155 pKa = 9.88EE156 pKa = 4.34AYY158 pKa = 10.69LGANNMWMDD167 pKa = 3.35GDD169 pKa = 4.18KK170 pKa = 10.51IYY172 pKa = 10.7GIQEE176 pKa = 3.34SDD178 pKa = 3.75YY179 pKa = 10.12AVIYY183 pKa = 9.04TGVWDD188 pKa = 4.57GVSADD193 pKa = 3.49TVFYY197 pKa = 10.93QMSEE201 pKa = 4.04DD202 pKa = 3.88ADD204 pKa = 3.64QEE206 pKa = 4.37FYY208 pKa = 10.92LYY210 pKa = 10.45HH211 pKa = 6.45GCLVQASYY219 pKa = 11.91ANDD222 pKa = 3.0ILQRR226 pKa = 11.84GEE228 pKa = 4.21VVTLCRR234 pKa = 11.84LTDD237 pKa = 3.57NTIKK241 pKa = 11.05LLDD244 pKa = 3.61KK245 pKa = 11.2

Molecular weight: 28.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2IAV7|A0A1B2IAV7_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Caitlin OX=1883379 GN=CAITLIN_81 PE=4 SV=1
MM1 pKa = 8.09RR2 pKa = 11.84YY3 pKa = 8.62HH4 pKa = 6.74HH5 pKa = 6.51VATRR9 pKa = 11.84SAKK12 pKa = 8.67EE13 pKa = 3.68TFLIHH18 pKa = 6.78LSYY21 pKa = 11.04ILTEE25 pKa = 4.43LNRR28 pKa = 11.84KK29 pKa = 4.34TTQRR33 pKa = 11.84EE34 pKa = 3.95LAQLCQVSVSTLGAVKK50 pKa = 10.44NGRR53 pKa = 11.84SKK55 pKa = 10.94NVSFEE60 pKa = 4.0TMLRR64 pKa = 11.84IADD67 pKa = 4.07AVRR70 pKa = 11.84LTYY73 pKa = 10.21QVILSSKK80 pKa = 8.33HH81 pKa = 5.17GKK83 pKa = 9.39SKK85 pKa = 10.61FVVTCEE91 pKa = 4.27SGVEE95 pKa = 3.91YY96 pKa = 9.87MKK98 pKa = 10.79NSRR101 pKa = 11.84IRR103 pKa = 11.84ISDD106 pKa = 3.24HH107 pKa = 5.22GRR109 pKa = 11.84IMTARR114 pKa = 11.84RR115 pKa = 11.84VNHH118 pKa = 6.19

Molecular weight: 13.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

271

0

271

75845

35

2457

279.9

31.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.078 ± 0.159

0.891 ± 0.048

6.368 ± 0.1

6.185 ± 0.169

3.946 ± 0.071

6.582 ± 0.175

2.123 ± 0.076

5.488 ± 0.1

5.278 ± 0.127

9.084 ± 0.113

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.948 ± 0.068

4.926 ± 0.105

4.398 ± 0.102

3.726 ± 0.075

5.567 ± 0.131

5.568 ± 0.102

6.322 ± 0.156

7.159 ± 0.095

1.436 ± 0.06

3.926 ± 0.087
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski