Sediminihabitans luteus
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2995 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2M9D055|A0A2M9D055_9CELL Uncharacterized protein OS=Sediminihabitans luteus OX=1138585 GN=CLV28_0806 PE=4 SV=1
MM1 pKa = 6.85 PTHH4 pKa = 5.73 YY5 pKa = 10.14 RR6 pKa = 11.84 YY7 pKa = 9.96 GVSMRR12 pKa = 11.84 TSRR15 pKa = 11.84 PATVAVVVAALVTALLAGCDD35 pKa = 3.79 APGKK39 pKa = 9.33 EE40 pKa = 4.34 QTPTLDD46 pKa = 3.55 PSASSTQGADD56 pKa = 3.53 PLAGAPAGYY65 pKa = 9.9 EE66 pKa = 3.92 SYY68 pKa = 11.01 YY69 pKa = 10.88 GQTLEE74 pKa = 4.39 WEE76 pKa = 4.59 SCGGGYY82 pKa = 10.3 EE83 pKa = 4.38 CTDD86 pKa = 3.41 ADD88 pKa = 5.92 APISWQDD95 pKa = 3.2 PDD97 pKa = 4.26 LGSIQLALKK106 pKa = 10.02 RR107 pKa = 11.84 LPASGDD113 pKa = 3.48 RR114 pKa = 11.84 QGSLLINPGGPGSSGVEE131 pKa = 4.14 FVTSAASIIGDD142 pKa = 3.89 RR143 pKa = 11.84 VKK145 pKa = 10.79 GAYY148 pKa = 9.88 DD149 pKa = 3.44 LVGFDD154 pKa = 3.75 PRR156 pKa = 11.84 GVGGSAPVKK165 pKa = 10.7 CLDD168 pKa = 5.2 DD169 pKa = 4.36 ADD171 pKa = 4.6 KK172 pKa = 11.17 DD173 pKa = 3.88 ASLSASYY180 pKa = 9.94 PHH182 pKa = 5.69 TTEE185 pKa = 5.62 GIAAMADD192 pKa = 3.75 DD193 pKa = 4.69 LEE195 pKa = 5.26 AWAQACDD202 pKa = 3.83 EE203 pKa = 4.3 NTGEE207 pKa = 4.37 QLATVDD213 pKa = 3.81 TQSAARR219 pKa = 11.84 DD220 pKa = 3.49 MDD222 pKa = 3.72 MLRR225 pKa = 11.84 AVLGDD230 pKa = 3.73 EE231 pKa = 3.95 QLHH234 pKa = 5.52 YY235 pKa = 11.17 LGFSYY240 pKa = 8.67 GTQLGATYY248 pKa = 10.71 AGLFPQTVGRR258 pKa = 11.84 MVLDD262 pKa = 3.92 GAIDD266 pKa = 3.58 TTLDD270 pKa = 3.31 ADD272 pKa = 4.53 EE273 pKa = 5.19 ISAEE277 pKa = 3.97 QAVGFEE283 pKa = 4.25 NALRR287 pKa = 11.84 AYY289 pKa = 9.2 VTDD292 pKa = 3.9 CLGGRR297 pKa = 11.84 ACPLSGSVDD306 pKa = 2.83 GGMQQIRR313 pKa = 11.84 DD314 pKa = 3.76 LLDD317 pKa = 3.37 RR318 pKa = 11.84 TLEE321 pKa = 4.47 DD322 pKa = 4.48 PLPTDD327 pKa = 3.31 SDD329 pKa = 3.86 RR330 pKa = 11.84 DD331 pKa = 3.7 LTQSLTFYY339 pKa = 11.13 GIALPLYY346 pKa = 10.64 SKK348 pKa = 11.23 ANWSLLTNALSDD360 pKa = 3.76 ALEE363 pKa = 5.1 DD364 pKa = 4.3 GDD366 pKa = 5.63 GSTLLYY372 pKa = 10.81 LADD375 pKa = 5.28 FYY377 pKa = 11.77 NDD379 pKa = 3.72 RR380 pKa = 11.84 QPDD383 pKa = 3.58 GSFSSNSAEE392 pKa = 3.77 AFRR395 pKa = 11.84 AINCLDD401 pKa = 3.5 DD402 pKa = 5.18 RR403 pKa = 11.84 GNPDD407 pKa = 3.89 PDD409 pKa = 3.9 FMAEE413 pKa = 3.72 QAEE416 pKa = 4.71 EE417 pKa = 4.15 IEE419 pKa = 4.39 AAAPTMGEE427 pKa = 3.92 FFTYY431 pKa = 10.4 SGLTCADD438 pKa = 3.23 WPVPEE443 pKa = 5.07 VEE445 pKa = 4.37 QQFDD449 pKa = 3.43 LHH451 pKa = 7.65 ASGAAPIVVVGTTNDD466 pKa = 3.55 PATPYY471 pKa = 10.5 VWAQGLAKK479 pKa = 9.67 TLDD482 pKa = 3.54 SATLVTFEE490 pKa = 4.97 GEE492 pKa = 3.74 GHH494 pKa = 5.44 TAYY497 pKa = 10.31 GSSNDD502 pKa = 3.9 CVGDD506 pKa = 3.61 AVDD509 pKa = 5.52 DD510 pKa = 4.03 YY511 pKa = 11.55 LVDD514 pKa = 3.6 GTVPKK519 pKa = 10.82 DD520 pKa = 3.3 GLTCC524 pKa = 4.55
Molecular weight: 54.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.783
IPC_protein 3.834
Toseland 3.592
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.643
EMBOSS 3.77
Sillero 3.948
Patrickios 0.896
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.852
Protein with the highest isoelectric point:
>tr|A0A2M9CCW4|A0A2M9CCW4_9CELL Uncharacterized protein OS=Sediminihabitans luteus OX=1138585 GN=CLV28_2695 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 SILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.61 GRR40 pKa = 11.84 AEE42 pKa = 3.84 LSAA45 pKa = 4.93
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.453
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.34
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.735
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.076
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2995
0
2995
1036919
29
5544
346.2
36.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.55 ± 0.061
0.566 ± 0.01
6.909 ± 0.047
5.258 ± 0.042
2.495 ± 0.028
9.467 ± 0.045
2.081 ± 0.027
2.88 ± 0.032
1.519 ± 0.031
9.82 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.537 ± 0.019
1.487 ± 0.022
5.943 ± 0.047
2.464 ± 0.026
7.493 ± 0.061
5.092 ± 0.037
6.756 ± 0.069
10.388 ± 0.062
1.499 ± 0.019
1.796 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here