Salinibacter phage M8CC-19
Average proteome isoelectric point is 5.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I6UG82|A0A2I6UG82_9CAUD Uncharacterized protein OS=Salinibacter phage M8CC-19 OX=2681613 PE=4 SV=1
MM1 pKa = 7.48 IKK3 pKa = 10.41 LFEE6 pKa = 4.56 FLPEE10 pKa = 4.25 DD11 pKa = 4.32 YY12 pKa = 10.37 EE13 pKa = 4.4 WVDD16 pKa = 3.13 APLYY20 pKa = 10.43 VEE22 pKa = 5.42 AGFTLHH28 pKa = 6.87 EE29 pKa = 4.47 GRR31 pKa = 11.84 PARR34 pKa = 11.84 MNYY37 pKa = 10.09 GGLPDD42 pKa = 4.12 PAEE45 pKa = 4.14 PATYY49 pKa = 9.98 EE50 pKa = 3.89 VDD52 pKa = 3.2 EE53 pKa = 4.5 LRR55 pKa = 11.84 IEE57 pKa = 4.37 EE58 pKa = 4.43 GPKK61 pKa = 10.35 LSEE64 pKa = 4.19 AVDD67 pKa = 3.87 DD68 pKa = 4.04 EE69 pKa = 4.58 TFEE72 pKa = 4.22 NVLDD76 pKa = 4.47 VIYY79 pKa = 7.15 EE80 pKa = 4.38 TPVHH84 pKa = 6.3 EE85 pKa = 4.26 VAEE88 pKa = 4.5 MYY90 pKa = 10.65 VRR92 pKa = 4.25
Molecular weight: 10.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.028
IPC2_protein 4.062
IPC_protein 3.961
Toseland 3.795
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.783
Rodwell 3.795
Grimsley 3.719
Solomon 3.897
Lehninger 3.846
Nozaki 4.024
DTASelect 4.139
Thurlkill 3.821
EMBOSS 3.795
Sillero 4.075
Patrickios 2.842
IPC_peptide 3.897
IPC2_peptide 4.062
IPC2.peptide.svr19 3.97
Protein with the highest isoelectric point:
>tr|A0A2I6UG90|A0A2I6UG90_9CAUD Uncharacterized protein OS=Salinibacter phage M8CC-19 OX=2681613 PE=4 SV=1
MM1 pKa = 8.03 PILPPSTGRR10 pKa = 11.84 TPEE13 pKa = 4.14 DD14 pKa = 3.14 KK15 pKa = 10.78 KK16 pKa = 10.55 QLRR19 pKa = 11.84 DD20 pKa = 3.63 LLSWSARR27 pKa = 11.84 HH28 pKa = 6.0 GFGPKK33 pKa = 10.29 FGTTTVSHH41 pKa = 5.71 YY42 pKa = 11.11 QNTHH46 pKa = 4.37 FTRR49 pKa = 11.84 LKK51 pKa = 10.84 GLGALIGIYY60 pKa = 10.24 DD61 pKa = 4.2 PSRR64 pKa = 11.84 SRR66 pKa = 11.84 WTGRR70 pKa = 11.84 NAEE73 pKa = 3.9 VRR75 pKa = 11.84 VRR77 pKa = 11.84 DD78 pKa = 3.65 GEE80 pKa = 3.85 IQYY83 pKa = 11.28 RR84 pKa = 11.84 LFSPKK89 pKa = 9.81 GDD91 pKa = 3.47 APEE94 pKa = 5.06 KK95 pKa = 7.91 FTRR98 pKa = 11.84 WNVAANAEE106 pKa = 4.09 KK107 pKa = 9.99 FARR110 pKa = 11.84 FGRR113 pKa = 11.84 RR114 pKa = 11.84 SVRR117 pKa = 11.84 YY118 pKa = 8.95
Molecular weight: 13.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 9.94
IPC_protein 10.979
Toseland 11.052
ProMoST 11.096
Dawson 11.111
Bjellqvist 10.921
Wikipedia 11.418
Rodwell 11.199
Grimsley 11.155
Solomon 11.374
Lehninger 11.316
Nozaki 11.038
DTASelect 10.921
Thurlkill 11.052
EMBOSS 11.491
Sillero 11.067
Patrickios 10.921
IPC_peptide 11.374
IPC2_peptide 10.058
IPC2.peptide.svr19 8.561
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
75
0
75
15665
31
2773
208.9
23.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.494 ± 0.405
0.44 ± 0.083
7.514 ± 0.281
8.171 ± 0.484
4.264 ± 0.26
7.603 ± 0.589
1.934 ± 0.22
5.062 ± 0.226
4.89 ± 0.316
7.124 ± 0.28
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.368 ± 0.306
4.832 ± 0.431
4.124 ± 0.249
3.205 ± 0.275
6.613 ± 0.417
5.956 ± 0.514
6.122 ± 0.325
7.105 ± 0.282
1.366 ± 0.12
3.811 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here