Arthrobacter phage YesChef
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G8R2V6|A0A6G8R2V6_9CAUD Uncharacterized protein OS=Arthrobacter phage YesChef OX=2713261 GN=31 PE=4 SV=1
MM1 pKa = 7.84 LEE3 pKa = 4.3 YY4 pKa = 10.84 LDD6 pKa = 5.91 DD7 pKa = 5.77 DD8 pKa = 5.26 PDD10 pKa = 5.47 LEE12 pKa = 5.09 LDD14 pKa = 3.26 PRR16 pKa = 11.84 EE17 pKa = 4.38 YY18 pKa = 11.11 VCEE21 pKa = 4.2 TCRR24 pKa = 11.84 LTHH27 pKa = 6.06 WRR29 pKa = 11.84 GAPDD33 pKa = 3.65 PCDD36 pKa = 3.41 RR37 pKa = 11.84 SS38 pKa = 3.51
Molecular weight: 4.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.826
IPC2_protein 4.101
IPC_protein 3.986
Toseland 3.783
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.706
Solomon 3.961
Lehninger 3.923
Nozaki 4.113
DTASelect 4.329
Thurlkill 3.859
EMBOSS 3.948
Sillero 4.113
Patrickios 1.952
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 3.989
Protein with the highest isoelectric point:
>tr|A0A6G8R305|A0A6G8R305_9CAUD Uncharacterized protein OS=Arthrobacter phage YesChef OX=2713261 GN=65 PE=4 SV=1
MM1 pKa = 7.51 PRR3 pKa = 11.84 KK4 pKa = 8.7 KK5 pKa = 9.21 TKK7 pKa = 10.15 TNLPADD13 pKa = 3.55 WHH15 pKa = 6.02 EE16 pKa = 4.52 HH17 pKa = 6.55 DD18 pKa = 4.07 SAEE21 pKa = 4.27 ANGRR25 pKa = 11.84 HH26 pKa = 4.91 ITAGTEE32 pKa = 3.6 VSIRR36 pKa = 11.84 GEE38 pKa = 3.62 RR39 pKa = 11.84 GRR41 pKa = 11.84 FRR43 pKa = 11.84 FLKK46 pKa = 10.33 RR47 pKa = 11.84 VTRR50 pKa = 11.84 DD51 pKa = 3.74 DD52 pKa = 3.36 GRR54 pKa = 11.84 EE55 pKa = 3.79 WLDD58 pKa = 3.17 FWGGPKK64 pKa = 10.27 GSEE67 pKa = 3.47 AWRR70 pKa = 11.84 SFSADD75 pKa = 3.0 QIRR78 pKa = 11.84 RR79 pKa = 11.84 VHH81 pKa = 7.36 RR82 pKa = 11.84 INTTEE87 pKa = 3.86 RR88 pKa = 11.84 ALAAQHH94 pKa = 6.09 KK95 pKa = 9.21 AKK97 pKa = 10.74 KK98 pKa = 9.0 EE99 pKa = 3.87 ALRR102 pKa = 11.84 NAA104 pKa = 3.94
Molecular weight: 12.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.56
IPC_protein 10.335
Toseland 11.067
ProMoST 11.008
Dawson 11.096
Bjellqvist 10.847
Wikipedia 11.359
Rodwell 11.228
Grimsley 11.111
Solomon 11.359
Lehninger 11.301
Nozaki 11.038
DTASelect 10.847
Thurlkill 11.038
EMBOSS 11.491
Sillero 11.038
Patrickios 10.979
IPC_peptide 11.359
IPC2_peptide 9.706
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
13389
36
990
194.0
21.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.578 ± 0.561
0.478 ± 0.093
6.475 ± 0.378
6.55 ± 0.409
3.077 ± 0.193
8.201 ± 0.487
1.875 ± 0.142
4.347 ± 0.323
4.25 ± 0.322
8.522 ± 0.337
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.875 ± 0.136
2.293 ± 0.241
5.139 ± 0.321
2.539 ± 0.231
7.342 ± 0.622
5.407 ± 0.278
6.573 ± 0.354
7.11 ± 0.262
1.748 ± 0.121
2.622 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here