Amino acid dipepetide frequency for Arthrobacter phage YesChef

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.943AlaAla: 19.943 ± 3.181
0.149AlaCys: 0.149 ± 0.11
9.561AlaAsp: 9.561 ± 1.198
9.187AlaGlu: 9.187 ± 0.974
4.033AlaPhe: 4.033 ± 0.568
9.486AlaGly: 9.486 ± 0.997
2.614AlaHis: 2.614 ± 0.475
5.677AlaIle: 5.677 ± 0.737
7.096AlaLys: 7.096 ± 0.716
13.146AlaLeu: 13.146 ± 0.979
2.838AlaMet: 2.838 ± 0.388
2.54AlaAsn: 2.54 ± 0.534
6.947AlaPro: 6.947 ± 0.727
3.735AlaGln: 3.735 ± 0.709
9.934AlaArg: 9.934 ± 1.004
7.469AlaSer: 7.469 ± 0.824
6.573AlaThr: 6.573 ± 0.647
9.635AlaVal: 9.635 ± 0.795
1.793AlaTrp: 1.793 ± 0.353
3.436AlaTyr: 3.436 ± 0.476
0.0AlaXaa: 0.0 ± 0.0
Cys
0.299CysAla: 0.299 ± 0.146
0.0CysCys: 0.0 ± 0.0
0.373CysAsp: 0.373 ± 0.159
0.448CysGlu: 0.448 ± 0.198
0.224CysPhe: 0.224 ± 0.117
0.523CysGly: 0.523 ± 0.211
0.075CysHis: 0.075 ± 0.08
0.224CysIle: 0.224 ± 0.118
0.224CysLys: 0.224 ± 0.133
0.0CysLeu: 0.0 ± 0.0
0.0CysMet: 0.0 ± 0.0
0.075CysAsn: 0.075 ± 0.075
0.523CysPro: 0.523 ± 0.199
0.373CysGln: 0.373 ± 0.174
0.598CysArg: 0.598 ± 0.231
0.149CysSer: 0.149 ± 0.102
0.224CysThr: 0.224 ± 0.135
0.075CysVal: 0.075 ± 0.067
0.075CysTrp: 0.075 ± 0.08
0.299CysTyr: 0.299 ± 0.154
0.0CysXaa: 0.0 ± 0.0
Asp
11.129AspAla: 11.129 ± 0.956
0.299AspCys: 0.299 ± 0.139
4.033AspAsp: 4.033 ± 0.525
4.033AspGlu: 4.033 ± 0.637
2.316AspPhe: 2.316 ± 0.356
5.677AspGly: 5.677 ± 0.889
1.344AspHis: 1.344 ± 0.365
1.718AspIle: 1.718 ± 0.33
2.39AspLys: 2.39 ± 0.437
6.797AspLeu: 6.797 ± 0.805
0.598AspMet: 0.598 ± 0.161
1.643AspAsn: 1.643 ± 0.48
3.959AspPro: 3.959 ± 0.533
1.27AspGln: 1.27 ± 0.291
2.988AspArg: 2.988 ± 0.601
3.212AspSer: 3.212 ± 0.446
3.884AspThr: 3.884 ± 0.431
4.78AspVal: 4.78 ± 0.637
1.12AspTrp: 1.12 ± 0.26
1.569AspTyr: 1.569 ± 0.381
0.0AspXaa: 0.0 ± 0.0
Glu
10.233GluAla: 10.233 ± 1.221
0.149GluCys: 0.149 ± 0.096
4.033GluAsp: 4.033 ± 0.572
5.378GluGlu: 5.378 ± 0.729
1.569GluPhe: 1.569 ± 0.277
4.332GluGly: 4.332 ± 0.505
1.643GluHis: 1.643 ± 0.352
3.884GluIle: 3.884 ± 0.582
2.166GluLys: 2.166 ± 0.492
5.826GluLeu: 5.826 ± 0.798
1.195GluMet: 1.195 ± 0.308
1.12GluAsn: 1.12 ± 0.304
2.764GluPro: 2.764 ± 0.485
0.971GluGln: 0.971 ± 0.224
5.378GluArg: 5.378 ± 1.058
3.361GluSer: 3.361 ± 0.51
3.66GluThr: 3.66 ± 0.611
4.706GluVal: 4.706 ± 0.568
1.569GluTrp: 1.569 ± 0.36
1.569GluTyr: 1.569 ± 0.323
0.0GluXaa: 0.0 ± 0.0
Phe
3.137PheAla: 3.137 ± 0.332
0.299PheCys: 0.299 ± 0.142
2.689PheAsp: 2.689 ± 0.591
2.091PheGlu: 2.091 ± 0.359
0.747PhePhe: 0.747 ± 0.218
2.913PheGly: 2.913 ± 0.402
0.598PheHis: 0.598 ± 0.214
1.419PheIle: 1.419 ± 0.337
1.419PheLys: 1.419 ± 0.318
2.091PheLeu: 2.091 ± 0.404
0.672PheMet: 0.672 ± 0.232
0.373PheAsn: 0.373 ± 0.152
1.419PhePro: 1.419 ± 0.25
1.046PheGln: 1.046 ± 0.305
1.793PheArg: 1.793 ± 0.342
1.867PheSer: 1.867 ± 0.281
2.54PheThr: 2.54 ± 0.55
2.166PheVal: 2.166 ± 0.463
0.448PheTrp: 0.448 ± 0.166
1.046PheTyr: 1.046 ± 0.245
0.0PheXaa: 0.0 ± 0.0
Gly
9.187GlyAla: 9.187 ± 0.924
0.299GlyCys: 0.299 ± 0.149
5.378GlyAsp: 5.378 ± 0.838
5.527GlyGlu: 5.527 ± 0.613
2.764GlyPhe: 2.764 ± 0.476
6.947GlyGly: 6.947 ± 0.903
1.643GlyHis: 1.643 ± 0.318
3.511GlyIle: 3.511 ± 0.975
4.706GlyLys: 4.706 ± 0.621
6.947GlyLeu: 6.947 ± 0.935
1.643GlyMet: 1.643 ± 0.318
2.39GlyAsn: 2.39 ± 0.593
3.361GlyPro: 3.361 ± 0.521
1.793GlyGln: 1.793 ± 0.387
5.751GlyArg: 5.751 ± 0.722
4.033GlySer: 4.033 ± 0.65
4.631GlyThr: 4.631 ± 0.825
5.826GlyVal: 5.826 ± 0.755
2.017GlyTrp: 2.017 ± 0.448
3.66GlyTyr: 3.66 ± 0.481
0.0GlyXaa: 0.0 ± 0.0
His
2.39HisAla: 2.39 ± 0.477
0.224HisCys: 0.224 ± 0.131
1.195HisAsp: 1.195 ± 0.27
1.419HisGlu: 1.419 ± 0.412
0.822HisPhe: 0.822 ± 0.277
1.643HisGly: 1.643 ± 0.302
0.598HisHis: 0.598 ± 0.211
0.299HisIle: 0.299 ± 0.132
0.448HisLys: 0.448 ± 0.174
2.166HisLeu: 2.166 ± 0.372
0.224HisMet: 0.224 ± 0.142
0.448HisAsn: 0.448 ± 0.161
1.494HisPro: 1.494 ± 0.293
0.299HisGln: 0.299 ± 0.168
1.12HisArg: 1.12 ± 0.284
1.046HisSer: 1.046 ± 0.276
1.195HisThr: 1.195 ± 0.239
1.12HisVal: 1.12 ± 0.26
0.224HisTrp: 0.224 ± 0.164
0.373HisTyr: 0.373 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
5.303IleAla: 5.303 ± 0.727
0.149IleCys: 0.149 ± 0.108
2.316IleAsp: 2.316 ± 0.415
3.585IleGlu: 3.585 ± 0.596
1.046IlePhe: 1.046 ± 0.342
4.108IleGly: 4.108 ± 0.541
0.896IleHis: 0.896 ± 0.236
1.867IleIle: 1.867 ± 0.417
1.569IleLys: 1.569 ± 0.42
2.913IleLeu: 2.913 ± 0.596
0.672IleMet: 0.672 ± 0.228
1.344IleAsn: 1.344 ± 0.323
2.465IlePro: 2.465 ± 0.453
2.465IleGln: 2.465 ± 0.582
3.585IleArg: 3.585 ± 0.387
2.017IleSer: 2.017 ± 0.423
2.54IleThr: 2.54 ± 0.48
3.212IleVal: 3.212 ± 0.584
0.822IleTrp: 0.822 ± 0.229
0.598IleTyr: 0.598 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
7.768LysAla: 7.768 ± 1.015
0.149LysCys: 0.149 ± 0.107
1.942LysAsp: 1.942 ± 0.453
2.838LysGlu: 2.838 ± 0.431
1.046LysPhe: 1.046 ± 0.285
3.287LysGly: 3.287 ± 0.435
1.046LysHis: 1.046 ± 0.322
2.316LysIle: 2.316 ± 0.366
2.017LysLys: 2.017 ± 0.574
3.212LysLeu: 3.212 ± 0.43
0.896LysMet: 0.896 ± 0.266
1.046LysAsn: 1.046 ± 0.276
2.166LysPro: 2.166 ± 0.413
1.12LysGln: 1.12 ± 0.297
3.062LysArg: 3.062 ± 0.494
1.494LysSer: 1.494 ± 0.338
2.913LysThr: 2.913 ± 0.516
2.913LysVal: 2.913 ± 0.373
0.747LysTrp: 0.747 ± 0.244
0.523LysTyr: 0.523 ± 0.178
0.0LysXaa: 0.0 ± 0.0
Leu
12.549LeuAla: 12.549 ± 1.246
0.523LeuCys: 0.523 ± 0.194
5.602LeuAsp: 5.602 ± 0.657
3.287LeuGlu: 3.287 ± 0.455
2.316LeuPhe: 2.316 ± 0.438
7.32LeuGly: 7.32 ± 1.056
1.942LeuHis: 1.942 ± 0.488
4.108LeuIle: 4.108 ± 0.467
2.465LeuLys: 2.465 ± 0.434
6.424LeuLeu: 6.424 ± 0.922
1.27LeuMet: 1.27 ± 0.418
2.764LeuAsn: 2.764 ± 0.362
4.332LeuPro: 4.332 ± 0.502
2.913LeuGln: 2.913 ± 0.547
7.245LeuArg: 7.245 ± 0.817
4.706LeuSer: 4.706 ± 0.597
6.498LeuThr: 6.498 ± 0.692
5.901LeuVal: 5.901 ± 0.549
1.643LeuTrp: 1.643 ± 0.298
1.344LeuTyr: 1.344 ± 0.241
0.0LeuXaa: 0.0 ± 0.0
Met
3.062MetAla: 3.062 ± 0.458
0.149MetCys: 0.149 ± 0.106
1.419MetAsp: 1.419 ± 0.311
0.822MetGlu: 0.822 ± 0.206
0.224MetPhe: 0.224 ± 0.124
1.046MetGly: 1.046 ± 0.383
0.075MetHis: 0.075 ± 0.066
0.822MetIle: 0.822 ± 0.294
1.27MetLys: 1.27 ± 0.348
1.494MetLeu: 1.494 ± 0.286
0.523MetMet: 0.523 ± 0.226
0.299MetAsn: 0.299 ± 0.143
1.419MetPro: 1.419 ± 0.258
0.149MetGln: 0.149 ± 0.099
1.12MetArg: 1.12 ± 0.254
1.718MetSer: 1.718 ± 0.304
1.867MetThr: 1.867 ± 0.366
0.971MetVal: 0.971 ± 0.289
0.0MetTrp: 0.0 ± 0.0
0.299MetTyr: 0.299 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
2.988AsnAla: 2.988 ± 0.505
0.0AsnCys: 0.0 ± 0.0
1.195AsnAsp: 1.195 ± 0.263
0.822AsnGlu: 0.822 ± 0.234
0.672AsnPhe: 0.672 ± 0.227
3.137AsnGly: 3.137 ± 0.436
0.299AsnHis: 0.299 ± 0.148
0.747AsnIle: 0.747 ± 0.207
0.523AsnLys: 0.523 ± 0.156
2.166AsnLeu: 2.166 ± 0.461
0.523AsnMet: 0.523 ± 0.2
0.523AsnAsn: 0.523 ± 0.294
1.718AsnPro: 1.718 ± 0.325
0.523AsnGln: 0.523 ± 0.198
1.344AsnArg: 1.344 ± 0.285
0.971AsnSer: 0.971 ± 0.305
1.569AsnThr: 1.569 ± 0.38
2.241AsnVal: 2.241 ± 0.62
0.598AsnTrp: 0.598 ± 0.175
0.373AsnTyr: 0.373 ± 0.139
0.0AsnXaa: 0.0 ± 0.0
Pro
6.722ProAla: 6.722 ± 0.981
0.598ProCys: 0.598 ± 0.228
4.482ProAsp: 4.482 ± 0.607
4.183ProGlu: 4.183 ± 0.584
2.091ProPhe: 2.091 ± 0.395
4.108ProGly: 4.108 ± 0.623
0.822ProHis: 0.822 ± 0.252
1.793ProIle: 1.793 ± 0.344
2.764ProLys: 2.764 ± 0.603
4.258ProLeu: 4.258 ± 0.764
1.12ProMet: 1.12 ± 0.307
0.971ProAsn: 0.971 ± 0.258
1.867ProPro: 1.867 ± 0.495
1.569ProGln: 1.569 ± 0.303
3.062ProArg: 3.062 ± 0.363
2.316ProSer: 2.316 ± 0.398
2.913ProThr: 2.913 ± 0.488
4.033ProVal: 4.033 ± 0.574
0.896ProTrp: 0.896 ± 0.273
0.822ProTyr: 0.822 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
2.689GlnAla: 2.689 ± 0.58
0.224GlnCys: 0.224 ± 0.122
1.419GlnAsp: 1.419 ± 0.4
1.569GlnGlu: 1.569 ± 0.362
0.598GlnPhe: 0.598 ± 0.209
1.942GlnGly: 1.942 ± 0.435
0.598GlnHis: 0.598 ± 0.19
2.017GlnIle: 2.017 ± 0.442
1.718GlnLys: 1.718 ± 0.367
1.419GlnLeu: 1.419 ± 0.384
0.971GlnMet: 0.971 ± 0.327
0.598GlnAsn: 0.598 ± 0.231
0.672GlnPro: 0.672 ± 0.234
0.523GlnGln: 0.523 ± 0.174
2.241GlnArg: 2.241 ± 0.458
1.494GlnSer: 1.494 ± 0.324
2.465GlnThr: 2.465 ± 0.567
1.494GlnVal: 1.494 ± 0.353
0.299GlnTrp: 0.299 ± 0.157
0.448GlnTyr: 0.448 ± 0.156
0.0GlnXaa: 0.0 ± 0.0
Arg
7.544ArgAla: 7.544 ± 0.986
0.224ArgCys: 0.224 ± 0.132
4.855ArgAsp: 4.855 ± 0.718
5.378ArgGlu: 5.378 ± 0.798
2.241ArgPhe: 2.241 ± 0.438
4.631ArgGly: 4.631 ± 0.738
1.27ArgHis: 1.27 ± 0.373
3.137ArgIle: 3.137 ± 0.524
3.287ArgLys: 3.287 ± 0.464
7.992ArgLeu: 7.992 ± 0.835
1.569ArgMet: 1.569 ± 0.33
1.569ArgAsn: 1.569 ± 0.284
3.062ArgPro: 3.062 ± 0.441
1.718ArgGln: 1.718 ± 0.373
6.872ArgArg: 6.872 ± 1.094
4.556ArgSer: 4.556 ± 0.576
4.556ArgThr: 4.556 ± 0.618
5.004ArgVal: 5.004 ± 0.733
1.494ArgTrp: 1.494 ± 0.331
2.465ArgTyr: 2.465 ± 0.451
0.0ArgXaa: 0.0 ± 0.0
Ser
6.274SerAla: 6.274 ± 0.602
0.299SerCys: 0.299 ± 0.153
2.764SerAsp: 2.764 ± 0.375
3.585SerGlu: 3.585 ± 0.505
2.39SerPhe: 2.39 ± 0.357
6.2SerGly: 6.2 ± 0.881
0.299SerHis: 0.299 ± 0.152
2.764SerIle: 2.764 ± 0.535
1.718SerLys: 1.718 ± 0.458
3.212SerLeu: 3.212 ± 0.442
1.195SerMet: 1.195 ± 0.305
1.494SerAsn: 1.494 ± 0.359
2.913SerPro: 2.913 ± 0.515
1.046SerGln: 1.046 ± 0.276
3.436SerArg: 3.436 ± 0.507
2.614SerSer: 2.614 ± 0.393
5.229SerThr: 5.229 ± 0.922
3.66SerVal: 3.66 ± 0.533
0.971SerTrp: 0.971 ± 0.223
2.017SerTyr: 2.017 ± 0.461
0.0SerXaa: 0.0 ± 0.0
Thr
8.963ThrAla: 8.963 ± 1.171
0.448ThrCys: 0.448 ± 0.255
3.735ThrAsp: 3.735 ± 0.434
4.258ThrGlu: 4.258 ± 0.623
2.241ThrPhe: 2.241 ± 0.387
6.2ThrGly: 6.2 ± 0.709
0.747ThrHis: 0.747 ± 0.237
2.838ThrIle: 2.838 ± 0.534
2.614ThrLys: 2.614 ± 0.347
6.2ThrLeu: 6.2 ± 0.519
1.046ThrMet: 1.046 ± 0.324
0.971ThrAsn: 0.971 ± 0.286
4.033ThrPro: 4.033 ± 0.565
1.12ThrGln: 1.12 ± 0.334
4.108ThrArg: 4.108 ± 0.694
4.033ThrSer: 4.033 ± 0.55
4.407ThrThr: 4.407 ± 0.57
5.453ThrVal: 5.453 ± 0.766
0.523ThrTrp: 0.523 ± 0.172
1.793ThrTyr: 1.793 ± 0.452
0.0ThrXaa: 0.0 ± 0.0
Val
10.084ValAla: 10.084 ± 0.902
0.448ValCys: 0.448 ± 0.204
5.229ValAsp: 5.229 ± 0.658
4.258ValGlu: 4.258 ± 0.735
2.241ValPhe: 2.241 ± 0.408
4.407ValGly: 4.407 ± 0.765
1.344ValHis: 1.344 ± 0.309
2.614ValIle: 2.614 ± 0.325
2.39ValLys: 2.39 ± 0.496
6.2ValLeu: 6.2 ± 0.632
1.12ValMet: 1.12 ± 0.228
1.569ValAsn: 1.569 ± 0.358
4.033ValPro: 4.033 ± 0.554
1.718ValGln: 1.718 ± 0.307
6.2ValArg: 6.2 ± 0.785
4.482ValSer: 4.482 ± 0.573
3.585ValThr: 3.585 ± 0.553
5.453ValVal: 5.453 ± 0.673
1.494ValTrp: 1.494 ± 0.337
2.241ValTyr: 2.241 ± 0.332
0.0ValXaa: 0.0 ± 0.0
Trp
1.867TrpAla: 1.867 ± 0.322
0.075TrpCys: 0.075 ± 0.064
1.12TrpAsp: 1.12 ± 0.247
1.494TrpGlu: 1.494 ± 0.328
0.224TrpPhe: 0.224 ± 0.117
1.27TrpGly: 1.27 ± 0.324
0.373TrpHis: 0.373 ± 0.176
0.971TrpIle: 0.971 ± 0.233
0.672TrpLys: 0.672 ± 0.204
0.822TrpLeu: 0.822 ± 0.235
0.149TrpMet: 0.149 ± 0.106
0.747TrpAsn: 0.747 ± 0.268
0.672TrpPro: 0.672 ± 0.229
0.598TrpGln: 0.598 ± 0.192
2.166TrpArg: 2.166 ± 0.347
1.419TrpSer: 1.419 ± 0.419
1.569TrpThr: 1.569 ± 0.39
0.523TrpVal: 0.523 ± 0.163
0.149TrpTrp: 0.149 ± 0.107
0.598TrpTyr: 0.598 ± 0.212
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.66TyrAla: 3.66 ± 0.482
0.075TyrCys: 0.075 ± 0.071
1.419TyrAsp: 1.419 ± 0.321
1.344TyrGlu: 1.344 ± 0.333
0.971TyrPhe: 0.971 ± 0.257
2.838TyrGly: 2.838 ± 0.461
0.523TyrHis: 0.523 ± 0.164
0.747TyrIle: 0.747 ± 0.23
1.046TyrLys: 1.046 ± 0.369
1.942TyrLeu: 1.942 ± 0.406
0.448TyrMet: 0.448 ± 0.184
0.448TyrAsn: 0.448 ± 0.193
1.569TyrPro: 1.569 ± 0.343
0.448TyrGln: 0.448 ± 0.171
1.419TyrArg: 1.419 ± 0.323
1.12TyrSer: 1.12 ± 0.305
2.913TyrThr: 2.913 ± 0.459
1.942TyrVal: 1.942 ± 0.501
0.598TyrTrp: 0.598 ± 0.187
0.747TyrTyr: 0.747 ± 0.292
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (13389 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski