Erythrobacter mangrovi
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2957 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D4BWM7|A0A7D4BWM7_9SPHN Uncharacterized protein OS=Erythrobacter mangrovi OX=2739433 GN=HQR01_11990 PE=4 SV=1
MM1 pKa = 7.36 RR2 pKa = 11.84 RR3 pKa = 11.84 IVSVLALPLMFASTAAWAQDD23 pKa = 3.53 DD24 pKa = 4.2 EE25 pKa = 5.53 GEE27 pKa = 4.27 EE28 pKa = 4.27 STGAWEE34 pKa = 3.74 IDD36 pKa = 3.31 AEE38 pKa = 4.24 IGVLSDD44 pKa = 3.06 YY45 pKa = 10.64 RR46 pKa = 11.84 FRR48 pKa = 11.84 GISLSSKK55 pKa = 10.22 DD56 pKa = 3.75 PEE58 pKa = 4.14 VTAEE62 pKa = 3.78 VSVAHH67 pKa = 6.29 EE68 pKa = 4.28 SGFYY72 pKa = 10.34 AGVWASNVAINDD84 pKa = 3.83 GADD87 pKa = 3.55 DD88 pKa = 4.49 VEE90 pKa = 5.68 LDD92 pKa = 4.51 LYY94 pKa = 11.37 AGFAPEE100 pKa = 3.87 VGAVSFDD107 pKa = 3.66 FGAVYY112 pKa = 10.57 YY113 pKa = 9.14 LYY115 pKa = 10.2 PSNSEE120 pKa = 3.66 FNYY123 pKa = 10.26 IEE125 pKa = 4.14 FLASAGTTVGPATVTVGVAYY145 pKa = 10.15 APSQDD150 pKa = 4.34 NIGNQDD156 pKa = 2.95 NTYY159 pKa = 10.74 VYY161 pKa = 10.72 INGDD165 pKa = 3.93 LPIGDD170 pKa = 4.58 TPLSLHH176 pKa = 5.77 GQFGLEE182 pKa = 4.28 DD183 pKa = 3.6 GAFADD188 pKa = 5.47 SKK190 pKa = 11.29 RR191 pKa = 11.84 DD192 pKa = 3.41 WLLGASYY199 pKa = 11.14 DD200 pKa = 4.06 LGGGLTATLDD210 pKa = 3.86 YY211 pKa = 11.28 VDD213 pKa = 3.59 TARR216 pKa = 11.84 AYY218 pKa = 9.41 TSLGDD223 pKa = 3.72 ATVVASLAFAFF234 pKa = 4.64
Molecular weight: 24.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.668
IPC_protein 3.668
Toseland 3.452
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.617
Rodwell 3.503
Grimsley 3.363
Solomon 3.656
Lehninger 3.617
Nozaki 3.783
DTASelect 4.024
Thurlkill 3.516
EMBOSS 3.617
Sillero 3.795
Patrickios 0.439
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|A0A7D3XG26|A0A7D3XG26_9SPHN Uncharacterized protein OS=Erythrobacter mangrovi OX=2739433 GN=HQR01_00535 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 8.0 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.44 NLCAA44 pKa = 4.54
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.422
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2957
0
2957
933445
35
2138
315.7
34.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.607 ± 0.059
0.827 ± 0.016
6.044 ± 0.037
6.324 ± 0.039
3.695 ± 0.029
8.856 ± 0.043
2.006 ± 0.024
5.016 ± 0.031
3.167 ± 0.033
9.903 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.476 ± 0.023
2.544 ± 0.03
5.139 ± 0.033
3.123 ± 0.023
7.023 ± 0.047
5.247 ± 0.033
5.163 ± 0.033
7.136 ± 0.031
1.469 ± 0.019
2.234 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here