Oerskovia sp. Root918
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3618 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q9C8V5|A0A0Q9C8V5_9CELL PDZ domain-containing protein OS=Oerskovia sp. Root918 OX=1736607 GN=ASE27_08870 PE=3 SV=1
MM1 pKa = 7.42 ARR3 pKa = 11.84 STLSIKK9 pKa = 10.17 RR10 pKa = 11.84 RR11 pKa = 11.84 ALAAVAGGASLAIVLTACSGSGDD34 pKa = 3.79 GGDD37 pKa = 4.64 AGDD40 pKa = 4.35 TEE42 pKa = 5.21 AATADD47 pKa = 3.31 CSAYY51 pKa = 10.13 EE52 pKa = 4.12 EE53 pKa = 4.45 YY54 pKa = 11.32 GDD56 pKa = 4.89 LSGKK60 pKa = 7.19 TVSVYY65 pKa = 7.36 TTIVDD70 pKa = 4.0 TEE72 pKa = 4.42 AEE74 pKa = 4.2 QQQEE78 pKa = 4.38 SYY80 pKa = 11.2 KK81 pKa = 10.7 PFEE84 pKa = 5.16 DD85 pKa = 3.83 CTGATIKK92 pKa = 11.22 YY93 pKa = 8.16 EE94 pKa = 3.98 GSKK97 pKa = 10.36 EE98 pKa = 3.94 FEE100 pKa = 3.94 AQLPIRR106 pKa = 11.84 IQSGSAPDD114 pKa = 3.29 IAYY117 pKa = 9.39 IPQPGFLKK125 pKa = 10.82 SLVQDD130 pKa = 3.77 YY131 pKa = 10.44 PDD133 pKa = 5.3 AILPVGDD140 pKa = 3.71 AASANVDD147 pKa = 3.4 EE148 pKa = 6.18 FYY150 pKa = 10.83 TEE152 pKa = 3.38 SWRR155 pKa = 11.84 DD156 pKa = 3.39 YY157 pKa = 9.35 GTVDD161 pKa = 2.94 GTLYY165 pKa = 9.35 ATPLGANVKK174 pKa = 10.15 SFVWYY179 pKa = 9.88 SPAMFEE185 pKa = 4.41 DD186 pKa = 3.58 AGYY189 pKa = 9.6 EE190 pKa = 4.43 IPTTWDD196 pKa = 3.11 EE197 pKa = 5.09 LIALSDD203 pKa = 4.31 QIVEE207 pKa = 4.52 DD208 pKa = 4.27 NPDD211 pKa = 3.5 GEE213 pKa = 4.42 IKK215 pKa = 9.93 PWCAGIGSGEE225 pKa = 4.01 ATGWPATDD233 pKa = 2.82 WMEE236 pKa = 4.54 DD237 pKa = 3.36 VVLRR241 pKa = 11.84 TAGPEE246 pKa = 4.65 VYY248 pKa = 9.99 DD249 pKa = 2.9 QWVNHH254 pKa = 6.96 EE255 pKa = 4.64 IPFNDD260 pKa = 3.7 PQIAEE265 pKa = 4.15 ALATVGTVLKK275 pKa = 10.89 NDD277 pKa = 4.02 DD278 pKa = 4.18 YY279 pKa = 11.76 VNGGIGDD286 pKa = 3.76 VSSIATTEE294 pKa = 4.01 FTEE297 pKa = 4.68 GGLPILDD304 pKa = 4.05 GLCYY308 pKa = 8.61 MHH310 pKa = 6.93 RR311 pKa = 11.84 QASFYY316 pKa = 9.03 QANWPEE322 pKa = 4.18 GTTVAEE328 pKa = 4.93 DD329 pKa = 3.73 GDD331 pKa = 3.96 VFAFYY336 pKa = 10.51 FPGNDD341 pKa = 3.32 TEE343 pKa = 4.54 AKK345 pKa = 8.76 PVLGGGEE352 pKa = 4.21 FVVSFADD359 pKa = 3.87 RR360 pKa = 11.84 PEE362 pKa = 4.05 VQAFQAFLTSPEE374 pKa = 3.78 WSNAKK379 pKa = 8.46 ATATPQGWISANKK392 pKa = 9.91 EE393 pKa = 3.7 LDD395 pKa = 3.27 KK396 pKa = 11.47 SLLQSPIDD404 pKa = 3.55 QLSYY408 pKa = 11.67 GLLTDD413 pKa = 3.9 DD414 pKa = 4.27 SYY416 pKa = 12.3 VFRR419 pKa = 11.84 FDD421 pKa = 4.05 GSDD424 pKa = 3.31 MMPAAVGAGSFWTEE438 pKa = 3.47 MTEE441 pKa = 4.34 WIASDD446 pKa = 3.56 KK447 pKa = 11.3 SDD449 pKa = 3.65 TAVLDD454 pKa = 4.8 AIEE457 pKa = 4.77 ASWPTSS463 pKa = 3.11
Molecular weight: 49.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.783
IPC_protein 3.795
Toseland 3.592
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.923
Patrickios 1.863
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A0Q9C1Z8|A0A0Q9C1Z8_9CELL MarR family transcriptional regulator OS=Oerskovia sp. Root918 OX=1736607 GN=ASE27_07240 PE=4 SV=1
MM1 pKa = 7.49 AGEE4 pKa = 3.79 WTIRR8 pKa = 11.84 EE9 pKa = 4.37 LARR12 pKa = 11.84 KK13 pKa = 9.6 AGVSRR18 pKa = 11.84 KK19 pKa = 9.32 SVWAWIDD26 pKa = 3.28 AQGWARR32 pKa = 11.84 PVSGPWILDD41 pKa = 3.25 GEE43 pKa = 4.42 RR44 pKa = 11.84 ARR46 pKa = 11.84 LVLEE50 pKa = 4.58 RR51 pKa = 11.84 FEE53 pKa = 4.03 QTAPLRR59 pKa = 11.84 TPRR62 pKa = 11.84 EE63 pKa = 3.94 PVPCSIEE70 pKa = 3.85 GCEE73 pKa = 3.85 RR74 pKa = 11.84 TRR76 pKa = 11.84 AGLQDD81 pKa = 3.93 MCKK84 pKa = 8.57 MHH86 pKa = 5.4 YY87 pKa = 8.42 QRR89 pKa = 11.84 RR90 pKa = 11.84 LRR92 pKa = 11.84 TGRR95 pKa = 11.84 TEE97 pKa = 3.93 RR98 pKa = 11.84 SSGGDD103 pKa = 2.79 WQTAKK108 pKa = 8.47 THH110 pKa = 6.29 CPAGHH115 pKa = 6.9 EE116 pKa = 3.95 YY117 pKa = 10.35 RR118 pKa = 11.84 PEE120 pKa = 4.09 NIYY123 pKa = 10.56 RR124 pKa = 11.84 FPSDD128 pKa = 2.45 VGTRR132 pKa = 11.84 RR133 pKa = 11.84 RR134 pKa = 11.84 CRR136 pKa = 11.84 TCRR139 pKa = 11.84 IAQSSVSKK147 pKa = 10.79 KK148 pKa = 10.22 PSS150 pKa = 2.95
Molecular weight: 17.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.355
IPC_protein 10.014
Toseland 10.409
ProMoST 10.116
Dawson 10.526
Bjellqvist 10.277
Wikipedia 10.73
Rodwell 10.657
Grimsley 10.584
Solomon 10.643
Lehninger 10.613
Nozaki 10.482
DTASelect 10.248
Thurlkill 10.423
EMBOSS 10.818
Sillero 10.482
Patrickios 10.394
IPC_peptide 10.643
IPC2_peptide 9.633
IPC2.peptide.svr19 8.45
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3618
0
3618
1211724
37
2957
334.9
35.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.817 ± 0.063
0.539 ± 0.009
6.275 ± 0.034
5.576 ± 0.038
2.733 ± 0.026
9.482 ± 0.039
2.0 ± 0.021
3.292 ± 0.035
1.691 ± 0.03
10.238 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.599 ± 0.016
1.654 ± 0.023
5.876 ± 0.034
2.701 ± 0.021
7.211 ± 0.047
5.503 ± 0.029
6.464 ± 0.041
9.934 ± 0.046
1.526 ± 0.016
1.887 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here