Bettongia penicillata papillomavirus 1
Average proteome isoelectric point is 5.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D6N1C0|D6N1C0_9PAPI Replication protein E1 OS=Bettongia penicillata papillomavirus 1 OX=759701 GN=E1 PE=3 SV=1
MM1 pKa = 7.76 IGDD4 pKa = 5.04 RR5 pKa = 11.84 IDD7 pKa = 4.05 LKK9 pKa = 11.27 DD10 pKa = 3.58 IVLQLDD16 pKa = 3.62 QVVPTSEE23 pKa = 4.33 EE24 pKa = 3.87 EE25 pKa = 4.16 STTGDD30 pKa = 3.5 EE31 pKa = 4.7 VEE33 pKa = 4.16 HH34 pKa = 6.7 RR35 pKa = 11.84 PYY37 pKa = 10.71 KK38 pKa = 10.56 YY39 pKa = 10.31 YY40 pKa = 10.95 DD41 pKa = 3.51 VTVSCATCSRR51 pKa = 11.84 RR52 pKa = 11.84 ISFCILANPEE62 pKa = 4.17 TVSGFFNYY70 pKa = 9.47 FFEE73 pKa = 4.78 DD74 pKa = 3.46 VRR76 pKa = 11.84 PVCLPCVQKK85 pKa = 11.35 GEE87 pKa = 4.14 NGQQ90 pKa = 3.41
Molecular weight: 10.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.149
IPC2_protein 4.482
IPC_protein 4.355
Toseland 4.19
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.19
Rodwell 4.19
Grimsley 4.101
Solomon 4.304
Lehninger 4.253
Nozaki 4.418
DTASelect 4.584
Thurlkill 4.202
EMBOSS 4.215
Sillero 4.469
Patrickios 2.003
IPC_peptide 4.304
IPC2_peptide 4.457
IPC2.peptide.svr19 4.378
Protein with the highest isoelectric point:
>tr|D6N1C2|D6N1C2_9PAPI E4 (Fragment) OS=Bettongia penicillata papillomavirus 1 OX=759701 PE=4 SV=1
MM1 pKa = 7.0 EE2 pKa = 4.65 TLTARR7 pKa = 11.84 FDD9 pKa = 3.75 AVQGKK14 pKa = 9.99 LMDD17 pKa = 4.59 LFEE20 pKa = 6.07 KK21 pKa = 10.64 DD22 pKa = 3.25 SNDD25 pKa = 3.33 IVDD28 pKa = 3.65 HH29 pKa = 6.28 CEE31 pKa = 3.5 YY32 pKa = 10.28 WSLIRR37 pKa = 11.84 QEE39 pKa = 4.38 NIMMNFGRR47 pKa = 11.84 KK48 pKa = 9.09 KK49 pKa = 9.4 GLKK52 pKa = 9.44 TIGLQPLPLSAVSEE66 pKa = 4.32 AQAKK70 pKa = 9.31 IAIEE74 pKa = 3.92 MEE76 pKa = 4.39 LVVKK80 pKa = 8.06 TLKK83 pKa = 10.57 NSPFGDD89 pKa = 4.04 EE90 pKa = 4.23 KK91 pKa = 10.29 WTFSDD96 pKa = 3.35 TSYY99 pKa = 9.58 EE100 pKa = 4.43 TYY102 pKa = 10.45 VSPPQRR108 pKa = 11.84 CFKK111 pKa = 10.4 KK112 pKa = 10.51 LPYY115 pKa = 9.67 LVKK118 pKa = 10.33 IKK120 pKa = 10.46 HH121 pKa = 6.13 GPNMDD126 pKa = 3.53 NVDD129 pKa = 3.93 VYY131 pKa = 10.53 TGWGKK136 pKa = 10.1 IYY138 pKa = 10.61 KK139 pKa = 9.39 QDD141 pKa = 3.84 CQGEE145 pKa = 4.44 WTVVTGEE152 pKa = 4.17 TDD154 pKa = 3.06 EE155 pKa = 4.51 TGCFYY160 pKa = 11.38 VEE162 pKa = 4.95 DD163 pKa = 4.09 DD164 pKa = 3.77 CKK166 pKa = 11.17 VYY168 pKa = 11.25 YY169 pKa = 10.29 KK170 pKa = 11.01 VFDD173 pKa = 4.19 IDD175 pKa = 3.54 SHH177 pKa = 7.82 NYY179 pKa = 10.86 DD180 pKa = 3.38 MTFKK184 pKa = 10.91 HH185 pKa = 6.38 PLNSDD190 pKa = 4.14 FVRR193 pKa = 11.84 KK194 pKa = 5.96 THH196 pKa = 6.9 RR197 pKa = 11.84 GRR199 pKa = 11.84 GQGEE203 pKa = 4.1 QDD205 pKa = 3.7 STPQTPSSQPEE216 pKa = 3.77 RR217 pKa = 11.84 TKK219 pKa = 10.73 EE220 pKa = 3.86 RR221 pKa = 11.84 KK222 pKa = 7.15 TGPRR226 pKa = 11.84 VRR228 pKa = 11.84 TSGTYY233 pKa = 8.47 TPPVGPLGGAQPIPSSAPKK252 pKa = 8.32 EE253 pKa = 4.67 TPDD256 pKa = 3.55 GAHH259 pKa = 7.02 PGGGGVPRR267 pKa = 11.84 GVGGRR272 pKa = 11.84 CDD274 pKa = 3.42 QPGGSGGPRR283 pKa = 11.84 KK284 pKa = 9.68 RR285 pKa = 11.84 AWEE288 pKa = 3.93 EE289 pKa = 3.77 GGGGGEE295 pKa = 4.19 EE296 pKa = 4.5 EE297 pKa = 4.36 EE298 pKa = 5.02 GEE300 pKa = 4.46 APRR303 pKa = 11.84 TSKK306 pKa = 10.73 KK307 pKa = 9.91 PAGGTDD313 pKa = 3.26 RR314 pKa = 11.84 PLEE317 pKa = 4.34 GQTGGRR323 pKa = 11.84 PGEE326 pKa = 4.34 VTSTTQRR333 pKa = 11.84 DD334 pKa = 3.91 CRR336 pKa = 11.84 EE337 pKa = 4.44 GPSSCPVTCPVTCPEE352 pKa = 4.17 TSCPVTSCPVTSPEE366 pKa = 4.17 TQCPVTQCPVLLAQGDD382 pKa = 4.23 ANILKK387 pKa = 9.31 CWRR390 pKa = 11.84 YY391 pKa = 9.91 RR392 pKa = 11.84 LEE394 pKa = 4.29 TKK396 pKa = 10.41 HH397 pKa = 6.45 KK398 pKa = 9.12 STYY401 pKa = 10.22 KK402 pKa = 10.28 HH403 pKa = 6.83 ISSTWYY409 pKa = 5.34 WTAGGRR415 pKa = 11.84 GQGKK419 pKa = 8.64 HH420 pKa = 5.54 RR421 pKa = 11.84 MLIAFDD427 pKa = 3.82 SAQQEE432 pKa = 4.26 KK433 pKa = 11.21 DD434 pKa = 3.41 FLLLKK439 pKa = 10.1 LPKK442 pKa = 10.33 GVFVSKK448 pKa = 10.55 GWFQNLL454 pKa = 3.01
Molecular weight: 50.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.694
IPC2_protein 6.927
IPC_protein 6.839
Toseland 6.605
ProMoST 7.41
Dawson 7.497
Bjellqvist 7.834
Wikipedia 7.41
Rodwell 7.512
Grimsley 6.649
Solomon 7.541
Lehninger 7.556
Nozaki 8.156
DTASelect 7.688
Thurlkill 7.746
EMBOSS 7.746
Sillero 8.039
Patrickios 4.24
IPC_peptide 7.527
IPC2_peptide 7.176
IPC2.peptide.svr19 7.065
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2475
90
596
353.6
39.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.485 ± 0.466
2.626 ± 0.804
6.545 ± 0.265
7.111 ± 0.729
4.485 ± 0.443
7.232 ± 1.012
1.778 ± 0.151
4.121 ± 0.261
5.98 ± 0.94
7.879 ± 0.887
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.374 ± 0.293
4.121 ± 0.825
7.798 ± 1.375
3.96 ± 0.433
5.939 ± 0.623
7.273 ± 0.651
6.263 ± 0.562
6.626 ± 0.643
1.455 ± 0.289
2.949 ± 0.437
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here