Ornithinimicrobium sp. CNJ-824
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2650 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U7MS04|A0A1U7MS04_9MICO Sugar ABC transporter substrate-binding protein OS=Ornithinimicrobium sp. CNJ-824 OX=1904966 GN=BJF81_13485 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 9.78 STGRR6 pKa = 11.84 GAFVASLAALALGLSACGSDD26 pKa = 3.41 NGDD29 pKa = 3.4 GGGGGDD35 pKa = 3.49 AGGDD39 pKa = 3.15 SGEE42 pKa = 4.29 PIKK45 pKa = 11.16 VGMIADD51 pKa = 4.16 LTGATGDD58 pKa = 3.17 VGAPYY63 pKa = 10.58 NEE65 pKa = 3.83 GMLAYY70 pKa = 10.42 VDD72 pKa = 3.55 WRR74 pKa = 11.84 NEE76 pKa = 3.82 NGGVADD82 pKa = 4.93 RR83 pKa = 11.84 EE84 pKa = 4.16 IEE86 pKa = 4.81 AISNDD91 pKa = 3.22 YY92 pKa = 10.8 AYY94 pKa = 9.43 EE95 pKa = 4.23 VPQAEE100 pKa = 4.38 QLYY103 pKa = 9.7 KK104 pKa = 10.59 QYY106 pKa = 11.4 LNDD109 pKa = 3.44 GVVAVQGWGTGDD121 pKa = 3.33 TEE123 pKa = 4.26 ALHH126 pKa = 6.23 TKK128 pKa = 10.0 VADD131 pKa = 3.91 DD132 pKa = 4.63 EE133 pKa = 4.8 LPFMSGSFAEE143 pKa = 4.45 SLTDD147 pKa = 4.28 PEE149 pKa = 3.97 EE150 pKa = 4.24 APYY153 pKa = 11.02 NFVVAPTYY161 pKa = 10.37 SDD163 pKa = 3.4 QMRR166 pKa = 11.84 VALNWIAEE174 pKa = 4.13 DD175 pKa = 3.47 SGGGAEE181 pKa = 4.14 VAVFHH186 pKa = 7.1 HH187 pKa = 7.22 DD188 pKa = 3.63 SPFGQAPVADD198 pKa = 4.06 GEE200 pKa = 4.48 AWVSEE205 pKa = 4.11 EE206 pKa = 4.49 GLDD209 pKa = 5.07 LGYY212 pKa = 10.16 QAYY215 pKa = 10.4 AMPGGQQNYY224 pKa = 10.16 VGLLSQAQSQGAEE237 pKa = 3.95 YY238 pKa = 10.32 IVIQNVASPAAQVAKK253 pKa = 10.65 DD254 pKa = 3.33 IAAQNLDD261 pKa = 3.57 MKK263 pKa = 10.33 IVCLNWCGNEE273 pKa = 4.08 LFIDD277 pKa = 3.69 TAGADD282 pKa = 3.56 VAEE285 pKa = 4.38 GHH287 pKa = 6.41 MLVQPFAPITSEE299 pKa = 4.16 KK300 pKa = 10.45 AGHH303 pKa = 6.16 EE304 pKa = 4.1 EE305 pKa = 3.45 MTAYY309 pKa = 10.7 LEE311 pKa = 4.3 EE312 pKa = 4.29 QGEE315 pKa = 4.44 DD316 pKa = 3.46 PASKK320 pKa = 8.92 GTSWVQGWMVMHH332 pKa = 6.26 VMAEE336 pKa = 4.11 GMEE339 pKa = 4.19 KK340 pKa = 10.26 AAEE343 pKa = 4.55 DD344 pKa = 4.42 GEE346 pKa = 4.58 ITGPAIRR353 pKa = 11.84 EE354 pKa = 4.02 ALEE357 pKa = 3.9 TMGAIDD363 pKa = 3.5 TGGVIGEE370 pKa = 4.35 GNIEE374 pKa = 4.31 FSADD378 pKa = 3.05 SHH380 pKa = 6.85 RR381 pKa = 11.84 GSTSTGIYY389 pKa = 7.65 TAEE392 pKa = 4.23 GGEE395 pKa = 4.18 MVEE398 pKa = 4.54 VEE400 pKa = 4.95 AGATPP405 pKa = 3.93
Molecular weight: 42.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.884
IPC_protein 3.872
Toseland 3.681
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.719
Rodwell 3.694
Grimsley 3.592
Solomon 3.834
Lehninger 3.783
Nozaki 3.935
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.732
Sillero 3.986
Patrickios 1.329
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.871
Protein with the highest isoelectric point:
>tr|A0A1U7MN07|A0A1U7MN07_9MICO Uncharacterized protein OS=Ornithinimicrobium sp. CNJ-824 OX=1904966 GN=BJF81_07780 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 ARR15 pKa = 11.84 KK16 pKa = 8.65 HH17 pKa = 4.47 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILASRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.41 GRR40 pKa = 11.84 SSLSAA45 pKa = 3.62
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2650
0
2650
792233
37
1914
299.0
31.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.989 ± 0.063
0.658 ± 0.015
6.403 ± 0.044
6.071 ± 0.046
2.408 ± 0.028
9.681 ± 0.052
2.321 ± 0.026
2.657 ± 0.031
1.362 ± 0.028
10.612 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.888 ± 0.021
1.359 ± 0.021
6.033 ± 0.04
2.944 ± 0.026
8.321 ± 0.048
4.75 ± 0.03
6.08 ± 0.039
10.084 ± 0.05
1.654 ± 0.022
1.723 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here