Sediminibacillus albus
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3476 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G9CMM5|A0A1G9CMM5_9BACI Ribosomal silencing factor RsfS OS=Sediminibacillus albus OX=407036 GN=rsfS PE=3 SV=1
MM1 pKa = 7.85 EE2 pKa = 4.6 SQSLQGAQEE11 pKa = 3.99 LLCINTEE18 pKa = 3.94 KK19 pKa = 11.02 VYY21 pKa = 11.1 DD22 pKa = 3.88 WVILQANVSQNVLAAALGALPIDD45 pKa = 3.78 PCGPTVSNLTTTCFLVDD62 pKa = 3.73 PDD64 pKa = 4.31 TGEE67 pKa = 4.06 EE68 pKa = 4.23 LPPNAEE74 pKa = 3.63 ITIDD78 pKa = 3.6 EE79 pKa = 4.58 LGEE82 pKa = 4.17 RR83 pKa = 11.84 EE84 pKa = 4.47 DD85 pKa = 3.48 RR86 pKa = 11.84 TFVIDD91 pKa = 4.3 GSLVTLQRR99 pKa = 11.84 VTFTKK104 pKa = 9.58 TLSVVIEE111 pKa = 4.18 FSGLTGTTPFVEE123 pKa = 4.44 TTEE126 pKa = 4.99 PITIEE131 pKa = 3.77 IPEE134 pKa = 4.42 SLFLCAPEE142 pKa = 4.22 GTRR145 pKa = 11.84 IAVRR149 pKa = 11.84 LSDD152 pKa = 4.57 LDD154 pKa = 3.77 CSVNVNCVAAALTSVDD170 pKa = 3.3 IVLNICQSVQSLADD184 pKa = 3.39 VTIEE188 pKa = 4.4 LVADD192 pKa = 4.68 FCQPRR197 pKa = 11.84 DD198 pKa = 3.76 VLVEE202 pKa = 3.79 QCPTPTIPPQCPVLFPGNCDD222 pKa = 3.9 DD223 pKa = 4.73 NEE225 pKa = 4.53 HH226 pKa = 6.62 GATTTHH232 pKa = 6.5 PTLTRR237 pKa = 11.84 QDD239 pKa = 3.73 TNNGG243 pKa = 3.31
Molecular weight: 26.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.617
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.643
Grimsley 3.528
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.088
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.923
Patrickios 0.769
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A1G9ABA7|A0A1G9ABA7_9BACI Protease I OS=Sediminibacillus albus OX=407036 GN=SAMN05216243_2481 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.12 KK14 pKa = 8.46 VHH16 pKa = 5.5 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MSTKK25 pKa = 10.06 NGRR28 pKa = 11.84 KK29 pKa = 8.49 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.749
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.486
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3476
0
3476
992148
29
1996
285.4
32.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.316 ± 0.041
0.635 ± 0.012
5.344 ± 0.033
7.618 ± 0.054
4.489 ± 0.038
6.973 ± 0.044
2.071 ± 0.021
7.585 ± 0.043
6.527 ± 0.044
9.749 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.718 ± 0.02
4.398 ± 0.031
3.646 ± 0.022
4.144 ± 0.028
4.015 ± 0.028
6.095 ± 0.03
5.293 ± 0.029
6.8 ± 0.036
1.06 ± 0.016
3.525 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here