Enterococcus phage vB_EfaS_EF1c55

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saphexavirus; unclassified Saphexavirus

Average proteome isoelectric point is 5.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1K721|A0A5C1K721_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS_EF1c55 OX=2601631 PE=4 SV=1
MM1 pKa = 7.91DD2 pKa = 4.84NSLSVYY8 pKa = 9.37EE9 pKa = 4.04VQILNTNTGCLDD21 pKa = 3.56VFLVTAISITFAYY34 pKa = 9.79INFSEE39 pKa = 4.29TFEE42 pKa = 4.75EE43 pKa = 5.32DD44 pKa = 3.04FHH46 pKa = 9.52DD47 pKa = 4.75DD48 pKa = 3.76GNVKK52 pKa = 10.05VIKK55 pKa = 10.7LEE57 pKa = 4.06EE58 pKa = 4.0FF59 pKa = 3.56

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1K823|A0A5C1K823_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS_EF1c55 OX=2601631 PE=4 SV=1
MM1 pKa = 7.96ASFLWYY7 pKa = 9.32TIINNRR13 pKa = 11.84KK14 pKa = 8.67GVLNSMLEE22 pKa = 4.19RR23 pKa = 11.84IKK25 pKa = 11.38GMLRR29 pKa = 11.84KK30 pKa = 9.09IASSNGLATAA40 pKa = 4.75

Molecular weight: 4.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

16340

37

1330

173.8

19.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.271 ± 0.428

0.704 ± 0.087

6.248 ± 0.212

9.051 ± 0.387

4.315 ± 0.203

6.01 ± 0.347

1.493 ± 0.105

6.408 ± 0.239

7.595 ± 0.322

8.409 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.736 ± 0.154

5.208 ± 0.204

2.913 ± 0.204

3.452 ± 0.25

4.125 ± 0.197

5.783 ± 0.214

6.059 ± 0.313

6.677 ± 0.239

1.334 ± 0.125

4.211 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski