Amino acid dipepetide frequency for Enterococcus phage vB_EfaS_EF1c55

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.937AlaAla: 5.937 ± 1.324
0.551AlaCys: 0.551 ± 0.178
4.039AlaAsp: 4.039 ± 0.526
6.243AlaGlu: 6.243 ± 0.549
2.571AlaPhe: 2.571 ± 0.398
4.223AlaGly: 4.223 ± 0.627
0.857AlaHis: 0.857 ± 0.308
5.631AlaIle: 5.631 ± 0.621
5.08AlaLys: 5.08 ± 0.761
6.61AlaLeu: 6.61 ± 0.676
2.632AlaMet: 2.632 ± 0.567
3.795AlaAsn: 3.795 ± 0.586
1.775AlaPro: 1.775 ± 0.329
1.959AlaGln: 1.959 ± 0.386
2.877AlaArg: 2.877 ± 0.291
4.835AlaSer: 4.835 ± 0.523
4.223AlaThr: 4.223 ± 0.546
4.957AlaVal: 4.957 ± 0.626
0.49AlaTrp: 0.49 ± 0.179
3.427AlaTyr: 3.427 ± 0.598
0.0AlaXaa: 0.0 ± 0.0
Cys
0.306CysAla: 0.306 ± 0.119
0.245CysCys: 0.245 ± 0.149
0.612CysAsp: 0.612 ± 0.189
0.612CysGlu: 0.612 ± 0.186
0.367CysPhe: 0.367 ± 0.15
0.49CysGly: 0.49 ± 0.226
0.122CysHis: 0.122 ± 0.087
0.367CysIle: 0.367 ± 0.188
0.367CysLys: 0.367 ± 0.204
0.551CysLeu: 0.551 ± 0.176
0.306CysMet: 0.306 ± 0.126
0.428CysAsn: 0.428 ± 0.142
0.0CysPro: 0.0 ± 0.0
0.49CysGln: 0.49 ± 0.188
0.367CysArg: 0.367 ± 0.147
0.428CysSer: 0.428 ± 0.161
0.306CysThr: 0.306 ± 0.179
0.49CysVal: 0.49 ± 0.16
0.0CysTrp: 0.0 ± 0.0
0.184CysTyr: 0.184 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
4.039AspAla: 4.039 ± 0.46
0.796AspCys: 0.796 ± 0.222
3.244AspAsp: 3.244 ± 0.437
5.569AspGlu: 5.569 ± 0.634
4.039AspPhe: 4.039 ± 0.482
4.162AspGly: 4.162 ± 0.634
0.428AspHis: 0.428 ± 0.163
4.407AspIle: 4.407 ± 0.578
4.101AspLys: 4.101 ± 0.485
5.937AspLeu: 5.937 ± 0.644
2.387AspMet: 2.387 ± 0.425
2.938AspAsn: 2.938 ± 0.471
1.469AspPro: 1.469 ± 0.257
1.163AspGln: 1.163 ± 0.3
2.081AspArg: 2.081 ± 0.376
3.427AspSer: 3.427 ± 0.416
4.468AspThr: 4.468 ± 0.668
3.795AspVal: 3.795 ± 0.454
1.102AspTrp: 1.102 ± 0.338
2.877AspTyr: 2.877 ± 0.344
0.0AspXaa: 0.0 ± 0.0
Glu
8.385GluAla: 8.385 ± 0.854
0.49GluCys: 0.49 ± 0.179
6.977GluAsp: 6.977 ± 0.717
9.67GluGlu: 9.67 ± 1.105
3.611GluPhe: 3.611 ± 0.543
5.447GluGly: 5.447 ± 0.635
0.857GluHis: 0.857 ± 0.231
4.345GluIle: 4.345 ± 0.578
5.386GluLys: 5.386 ± 0.713
7.406GluLeu: 7.406 ± 0.775
2.571GluMet: 2.571 ± 0.442
3.489GluAsn: 3.489 ± 0.517
2.02GluPro: 2.02 ± 0.332
3.305GluGln: 3.305 ± 0.498
4.468GluArg: 4.468 ± 0.457
5.447GluSer: 5.447 ± 0.54
4.407GluThr: 4.407 ± 0.625
6.916GluVal: 6.916 ± 0.631
1.836GluTrp: 1.836 ± 0.342
3.489GluTyr: 3.489 ± 0.534
0.0GluXaa: 0.0 ± 0.0
Phe
2.509PheAla: 2.509 ± 0.41
0.428PheCys: 0.428 ± 0.161
2.999PheAsp: 2.999 ± 0.449
3.427PheGlu: 3.427 ± 0.412
1.53PhePhe: 1.53 ± 0.321
2.815PheGly: 2.815 ± 0.382
0.796PheHis: 0.796 ± 0.219
3.366PheIle: 3.366 ± 0.573
3.121PheLys: 3.121 ± 0.41
3.489PheLeu: 3.489 ± 0.457
0.979PheMet: 0.979 ± 0.205
2.265PheAsn: 2.265 ± 0.392
1.53PhePro: 1.53 ± 0.327
1.285PheGln: 1.285 ± 0.259
1.591PheArg: 1.591 ± 0.26
2.387PheSer: 2.387 ± 0.529
2.999PheThr: 2.999 ± 0.305
2.693PheVal: 2.693 ± 0.409
0.673PheTrp: 0.673 ± 0.215
2.265PheTyr: 2.265 ± 0.538
0.0PheXaa: 0.0 ± 0.0
Gly
3.427GlyAla: 3.427 ± 0.472
0.367GlyCys: 0.367 ± 0.185
3.244GlyAsp: 3.244 ± 0.522
3.917GlyGlu: 3.917 ± 0.455
3.611GlyPhe: 3.611 ± 0.489
3.795GlyGly: 3.795 ± 0.875
1.102GlyHis: 1.102 ± 0.249
4.284GlyIle: 4.284 ± 0.631
5.325GlyLys: 5.325 ± 0.632
4.713GlyLeu: 4.713 ± 0.517
1.836GlyMet: 1.836 ± 0.317
3.611GlyAsn: 3.611 ± 0.498
0.061GlyPro: 0.061 ± 0.063
2.081GlyGln: 2.081 ± 0.443
2.754GlyArg: 2.754 ± 0.388
2.877GlySer: 2.877 ± 0.366
4.651GlyThr: 4.651 ± 0.819
4.223GlyVal: 4.223 ± 0.589
1.285GlyTrp: 1.285 ± 0.344
2.938GlyTyr: 2.938 ± 0.395
0.0GlyXaa: 0.0 ± 0.0
His
1.04HisAla: 1.04 ± 0.199
0.184HisCys: 0.184 ± 0.109
0.612HisAsp: 0.612 ± 0.227
1.346HisGlu: 1.346 ± 0.288
0.918HisPhe: 0.918 ± 0.245
0.857HisGly: 0.857 ± 0.199
0.306HisHis: 0.306 ± 0.145
1.53HisIle: 1.53 ± 0.273
1.04HisLys: 1.04 ± 0.305
0.979HisLeu: 0.979 ± 0.225
0.061HisMet: 0.061 ± 0.061
1.163HisAsn: 1.163 ± 0.203
0.612HisPro: 0.612 ± 0.21
0.551HisGln: 0.551 ± 0.216
1.04HisArg: 1.04 ± 0.24
0.551HisSer: 0.551 ± 0.213
0.367HisThr: 0.367 ± 0.151
1.04HisVal: 1.04 ± 0.191
0.061HisTrp: 0.061 ± 0.06
0.673HisTyr: 0.673 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
4.529IleAla: 4.529 ± 0.579
0.428IleCys: 0.428 ± 0.153
4.101IleAsp: 4.101 ± 0.48
5.447IleGlu: 5.447 ± 0.625
1.959IlePhe: 1.959 ± 0.447
3.55IleGly: 3.55 ± 0.37
1.591IleHis: 1.591 ± 0.334
3.121IleIle: 3.121 ± 0.535
5.202IleLys: 5.202 ± 0.608
4.284IleLeu: 4.284 ± 0.536
1.714IleMet: 1.714 ± 0.357
4.651IleAsn: 4.651 ± 0.46
2.387IlePro: 2.387 ± 0.336
3.366IleGln: 3.366 ± 0.543
2.571IleArg: 2.571 ± 0.466
3.672IleSer: 3.672 ± 0.43
3.856IleThr: 3.856 ± 0.765
3.917IleVal: 3.917 ± 0.519
0.673IleTrp: 0.673 ± 0.231
3.06IleTyr: 3.06 ± 0.442
0.0IleXaa: 0.0 ± 0.0
Lys
7.344LysAla: 7.344 ± 0.687
0.428LysCys: 0.428 ± 0.145
5.263LysAsp: 5.263 ± 0.437
6.916LysGlu: 6.916 ± 0.728
2.632LysPhe: 2.632 ± 0.407
5.019LysGly: 5.019 ± 0.516
1.469LysHis: 1.469 ± 0.339
3.489LysIle: 3.489 ± 0.422
6.12LysLys: 6.12 ± 0.797
6.426LysLeu: 6.426 ± 0.82
2.203LysMet: 2.203 ± 0.471
2.693LysAsn: 2.693 ± 0.428
3.121LysPro: 3.121 ± 0.49
2.693LysGln: 2.693 ± 0.425
3.427LysArg: 3.427 ± 0.54
3.733LysSer: 3.733 ± 0.488
4.039LysThr: 4.039 ± 0.466
5.569LysVal: 5.569 ± 0.486
0.857LysTrp: 0.857 ± 0.218
2.509LysTyr: 2.509 ± 0.425
0.0LysXaa: 0.0 ± 0.0
Leu
5.508LeuAla: 5.508 ± 0.641
0.428LeuCys: 0.428 ± 0.163
5.386LeuAsp: 5.386 ± 0.536
10.221LeuGlu: 10.221 ± 1.039
2.938LeuPhe: 2.938 ± 0.377
4.59LeuGly: 4.59 ± 0.66
1.04LeuHis: 1.04 ± 0.215
5.753LeuIle: 5.753 ± 0.712
6.243LeuLys: 6.243 ± 0.706
5.386LeuLeu: 5.386 ± 0.722
2.326LeuMet: 2.326 ± 0.328
4.468LeuAsn: 4.468 ± 0.517
2.754LeuPro: 2.754 ± 0.352
3.244LeuGln: 3.244 ± 0.473
3.244LeuArg: 3.244 ± 0.424
5.019LeuSer: 5.019 ± 0.471
6.732LeuThr: 6.732 ± 0.6
5.508LeuVal: 5.508 ± 0.644
0.979LeuTrp: 0.979 ± 0.263
2.326LeuTyr: 2.326 ± 0.43
0.0LeuXaa: 0.0 ± 0.0
Met
2.632MetAla: 2.632 ± 0.513
0.061MetCys: 0.061 ± 0.057
2.081MetAsp: 2.081 ± 0.405
3.06MetGlu: 3.06 ± 0.451
0.918MetPhe: 0.918 ± 0.263
1.04MetGly: 1.04 ± 0.264
0.184MetHis: 0.184 ± 0.097
1.346MetIle: 1.346 ± 0.298
2.203MetLys: 2.203 ± 0.377
2.815MetLeu: 2.815 ± 0.485
0.49MetMet: 0.49 ± 0.158
1.591MetAsn: 1.591 ± 0.255
1.04MetPro: 1.04 ± 0.194
0.796MetGln: 0.796 ± 0.238
1.591MetArg: 1.591 ± 0.313
1.591MetSer: 1.591 ± 0.283
1.53MetThr: 1.53 ± 0.281
1.346MetVal: 1.346 ± 0.244
0.245MetTrp: 0.245 ± 0.129
0.796MetTyr: 0.796 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
2.938AsnAla: 2.938 ± 0.445
0.367AsnCys: 0.367 ± 0.161
2.326AsnAsp: 2.326 ± 0.347
3.856AsnGlu: 3.856 ± 0.525
2.203AsnPhe: 2.203 ± 0.309
3.427AsnGly: 3.427 ± 0.514
1.04AsnHis: 1.04 ± 0.352
3.244AsnIle: 3.244 ± 0.41
4.713AsnLys: 4.713 ± 0.532
4.284AsnLeu: 4.284 ± 0.449
1.224AsnMet: 1.224 ± 0.277
3.183AsnAsn: 3.183 ± 0.588
2.938AsnPro: 2.938 ± 0.539
1.836AsnGln: 1.836 ± 0.359
2.448AsnArg: 2.448 ± 0.308
3.305AsnSer: 3.305 ± 0.405
2.693AsnThr: 2.693 ± 0.459
2.938AsnVal: 2.938 ± 0.519
0.612AsnTrp: 0.612 ± 0.21
2.509AsnTyr: 2.509 ± 0.46
0.0AsnXaa: 0.0 ± 0.0
Pro
1.836ProAla: 1.836 ± 0.369
0.061ProCys: 0.061 ± 0.049
1.591ProAsp: 1.591 ± 0.283
3.305ProGlu: 3.305 ± 0.526
1.591ProPhe: 1.591 ± 0.284
0.184ProGly: 0.184 ± 0.086
0.796ProHis: 0.796 ± 0.217
2.081ProIle: 2.081 ± 0.354
2.877ProLys: 2.877 ± 0.362
2.142ProLeu: 2.142 ± 0.34
0.918ProMet: 0.918 ± 0.249
1.897ProAsn: 1.897 ± 0.417
0.673ProPro: 0.673 ± 0.221
0.796ProGln: 0.796 ± 0.219
1.04ProArg: 1.04 ± 0.31
2.509ProSer: 2.509 ± 0.416
1.775ProThr: 1.775 ± 0.269
1.652ProVal: 1.652 ± 0.397
0.367ProTrp: 0.367 ± 0.164
1.04ProTyr: 1.04 ± 0.286
0.0ProXaa: 0.0 ± 0.0
Gln
2.754GlnAla: 2.754 ± 0.356
0.061GlnCys: 0.061 ± 0.06
1.959GlnAsp: 1.959 ± 0.364
3.121GlnGlu: 3.121 ± 0.673
0.979GlnPhe: 0.979 ± 0.277
2.509GlnGly: 2.509 ± 0.438
0.612GlnHis: 0.612 ± 0.214
1.714GlnIle: 1.714 ± 0.3
2.571GlnLys: 2.571 ± 0.383
3.244GlnLeu: 3.244 ± 0.45
0.918GlnMet: 0.918 ± 0.273
1.04GlnAsn: 1.04 ± 0.238
0.979GlnPro: 0.979 ± 0.189
1.775GlnGln: 1.775 ± 0.5
1.285GlnArg: 1.285 ± 0.256
2.02GlnSer: 2.02 ± 0.398
2.509GlnThr: 2.509 ± 0.345
2.509GlnVal: 2.509 ± 0.367
0.612GlnTrp: 0.612 ± 0.201
1.346GlnTyr: 1.346 ± 0.293
0.0GlnXaa: 0.0 ± 0.0
Arg
2.571ArgAla: 2.571 ± 0.459
0.306ArgCys: 0.306 ± 0.139
2.265ArgAsp: 2.265 ± 0.288
3.611ArgGlu: 3.611 ± 0.413
2.203ArgPhe: 2.203 ± 0.333
2.754ArgGly: 2.754 ± 0.447
0.673ArgHis: 0.673 ± 0.237
3.55ArgIle: 3.55 ± 0.607
2.754ArgLys: 2.754 ± 0.437
4.59ArgLeu: 4.59 ± 0.522
0.857ArgMet: 0.857 ± 0.258
1.775ArgAsn: 1.775 ± 0.267
1.408ArgPro: 1.408 ± 0.33
1.408ArgGln: 1.408 ± 0.256
1.897ArgArg: 1.897 ± 0.351
1.591ArgSer: 1.591 ± 0.408
1.897ArgThr: 1.897 ± 0.345
2.632ArgVal: 2.632 ± 0.342
0.612ArgTrp: 0.612 ± 0.175
1.897ArgTyr: 1.897 ± 0.347
0.0ArgXaa: 0.0 ± 0.0
Ser
3.183SerAla: 3.183 ± 0.532
0.306SerCys: 0.306 ± 0.126
3.611SerAsp: 3.611 ± 0.492
4.774SerGlu: 4.774 ± 0.483
2.754SerPhe: 2.754 ± 0.323
3.672SerGly: 3.672 ± 0.465
0.673SerHis: 0.673 ± 0.219
4.407SerIle: 4.407 ± 0.597
5.508SerLys: 5.508 ± 0.583
5.202SerLeu: 5.202 ± 0.603
1.285SerMet: 1.285 ± 0.265
3.244SerAsn: 3.244 ± 0.447
1.53SerPro: 1.53 ± 0.272
1.53SerGln: 1.53 ± 0.367
2.326SerArg: 2.326 ± 0.361
2.999SerSer: 2.999 ± 0.45
3.611SerThr: 3.611 ± 0.451
4.101SerVal: 4.101 ± 0.456
0.49SerTrp: 0.49 ± 0.157
2.632SerTyr: 2.632 ± 0.412
0.0SerXaa: 0.0 ± 0.0
Thr
5.569ThrAla: 5.569 ± 0.846
0.49ThrCys: 0.49 ± 0.187
4.162ThrAsp: 4.162 ± 0.523
3.611ThrGlu: 3.611 ± 0.427
3.978ThrPhe: 3.978 ± 0.478
4.101ThrGly: 4.101 ± 0.597
0.673ThrHis: 0.673 ± 0.215
3.795ThrIle: 3.795 ± 0.52
3.672ThrLys: 3.672 ± 0.396
5.202ThrLeu: 5.202 ± 0.508
1.408ThrMet: 1.408 ± 0.264
3.305ThrAsn: 3.305 ± 0.603
2.203ThrPro: 2.203 ± 0.381
1.652ThrGln: 1.652 ± 0.329
1.897ThrArg: 1.897 ± 0.314
3.611ThrSer: 3.611 ± 0.609
3.244ThrThr: 3.244 ± 0.486
4.651ThrVal: 4.651 ± 0.592
0.673ThrTrp: 0.673 ± 0.2
2.693ThrTyr: 2.693 ± 0.397
0.0ThrXaa: 0.0 ± 0.0
Val
4.59ValAla: 4.59 ± 0.57
0.49ValCys: 0.49 ± 0.205
4.039ValAsp: 4.039 ± 0.472
6.243ValGlu: 6.243 ± 0.681
2.693ValPhe: 2.693 ± 0.408
3.856ValGly: 3.856 ± 0.555
0.918ValHis: 0.918 ± 0.221
4.651ValIle: 4.651 ± 0.62
5.631ValLys: 5.631 ± 0.545
5.814ValLeu: 5.814 ± 0.683
1.53ValMet: 1.53 ± 0.302
3.427ValAsn: 3.427 ± 0.527
1.53ValPro: 1.53 ± 0.369
2.448ValGln: 2.448 ± 0.386
2.081ValArg: 2.081 ± 0.343
4.529ValSer: 4.529 ± 0.563
3.856ValThr: 3.856 ± 0.564
4.284ValVal: 4.284 ± 0.68
1.224ValTrp: 1.224 ± 0.244
2.938ValTyr: 2.938 ± 0.366
0.0ValXaa: 0.0 ± 0.0
Trp
0.857TrpAla: 0.857 ± 0.228
0.184TrpCys: 0.184 ± 0.12
1.224TrpAsp: 1.224 ± 0.272
1.714TrpGlu: 1.714 ± 0.332
0.49TrpPhe: 0.49 ± 0.172
1.224TrpGly: 1.224 ± 0.309
0.245TrpHis: 0.245 ± 0.108
0.979TrpIle: 0.979 ± 0.215
1.04TrpLys: 1.04 ± 0.204
0.673TrpLeu: 0.673 ± 0.188
0.061TrpMet: 0.061 ± 0.055
0.918TrpAsn: 0.918 ± 0.216
0.0TrpPro: 0.0 ± 0.0
0.49TrpGln: 0.49 ± 0.193
0.367TrpArg: 0.367 ± 0.138
0.796TrpSer: 0.796 ± 0.203
0.796TrpThr: 0.796 ± 0.245
0.673TrpVal: 0.673 ± 0.195
0.061TrpTrp: 0.061 ± 0.064
0.551TrpTyr: 0.551 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.754TyrAla: 2.754 ± 0.399
0.367TyrCys: 0.367 ± 0.184
2.754TyrAsp: 2.754 ± 0.466
3.856TyrGlu: 3.856 ± 0.644
1.163TyrPhe: 1.163 ± 0.278
2.387TyrGly: 2.387 ± 0.451
0.551TyrHis: 0.551 ± 0.202
2.02TyrIle: 2.02 ± 0.331
2.999TyrLys: 2.999 ± 0.462
4.345TyrLeu: 4.345 ± 0.519
1.591TyrMet: 1.591 ± 0.302
2.203TyrAsn: 2.203 ± 0.385
1.102TyrPro: 1.102 ± 0.249
1.652TyrGln: 1.652 ± 0.286
1.897TyrArg: 1.897 ± 0.381
2.509TyrSer: 2.509 ± 0.377
2.632TyrThr: 2.632 ± 0.393
2.877TyrVal: 2.877 ± 0.33
0.49TyrTrp: 0.49 ± 0.171
1.959TyrTyr: 1.959 ± 0.452
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (16340 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski