Desulfuromonas acetoxidans (strain DSM 684 / 11070)
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3204 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q1K3J6|Q1K3J6_DESA6 Hydroxylamine reductase OS=Desulfuromonas acetoxidans (strain DSM 684 / 11070) OX=281689 GN=hcp PE=3 SV=1
MM1 pKa = 7.98 RR2 pKa = 11.84 SLKK5 pKa = 9.15 MACPTLCVMLLTLCWSGLAAAYY27 pKa = 10.15 DD28 pKa = 4.35 PIIHH32 pKa = 6.21 SEE34 pKa = 4.61 FYY36 pKa = 10.47 YY37 pKa = 11.12 GPDD40 pKa = 3.37 SEE42 pKa = 4.72 VHH44 pKa = 5.4 VVGDD48 pKa = 3.72 MLSVPEE54 pKa = 4.75 FSLTGEE60 pKa = 4.6 GIDD63 pKa = 3.6 VVFSGLEE70 pKa = 3.64 EE71 pKa = 4.5 GGGVEE76 pKa = 4.58 KK77 pKa = 11.06 YY78 pKa = 10.64 AVLLEE83 pKa = 4.11 YY84 pKa = 10.79 DD85 pKa = 3.97 SGSADD90 pKa = 3.5 LRR92 pKa = 11.84 DD93 pKa = 3.79 ATVSLTDD100 pKa = 3.12 ATFFFDD106 pKa = 3.51 TSGGNEE112 pKa = 4.13 DD113 pKa = 3.9 FNFAVGMKK121 pKa = 10.41 GFDD124 pKa = 3.24 EE125 pKa = 4.49 SLISLAEE132 pKa = 4.28 GSSLSVDD139 pKa = 2.68 IASYY143 pKa = 9.72 IGYY146 pKa = 9.44 GIEE149 pKa = 4.07 VLRR152 pKa = 11.84 SSATINLDD160 pKa = 3.25 GGSTIDD166 pKa = 3.26 VSGSVEE172 pKa = 4.24 DD173 pKa = 4.49 PVTFPDD179 pKa = 3.61 SAPSRR184 pKa = 11.84 FGYY187 pKa = 10.12 GINAGTPGGYY197 pKa = 10.4 VNLSLDD203 pKa = 3.47 HH204 pKa = 6.75 GSAINVEE211 pKa = 4.34 TSAYY215 pKa = 10.14 AAGDD219 pKa = 3.69 TGTSSASSAGARR231 pKa = 11.84 LEE233 pKa = 4.21 GDD235 pKa = 3.97 VVTVALDD242 pKa = 3.42 NEE244 pKa = 4.62 STIRR248 pKa = 11.84 TVATRR253 pKa = 11.84 NIASDD258 pKa = 3.62 GVTVANARR266 pKa = 11.84 NLRR269 pKa = 11.84 LGSISNSTIAVAGNSLISAQAFAEE293 pKa = 4.21 EE294 pKa = 4.52 GAAYY298 pKa = 9.8 AYY300 pKa = 10.49 VLQTYY305 pKa = 10.42 GSGASEE311 pKa = 4.26 FLLSDD316 pKa = 3.54 STFSATATADD326 pKa = 3.56 GYY328 pKa = 11.83 ADD330 pKa = 3.59 ALGAYY335 pKa = 9.19 FSQPEE340 pKa = 3.9 VRR342 pKa = 11.84 ATLTRR347 pKa = 11.84 STIDD351 pKa = 2.72 VDD353 pKa = 5.04 AISSSYY359 pKa = 10.06 GARR362 pKa = 11.84 ASGFVGQSNSDD373 pKa = 2.98 VRR375 pKa = 11.84 LEE377 pKa = 4.08 MEE379 pKa = 4.29 GATIDD384 pKa = 4.9 VLAQTQGDD392 pKa = 3.97 FANATGVMIIGATNLDD408 pKa = 3.12 IDD410 pKa = 4.25 IADD413 pKa = 3.82 STVDD417 pKa = 3.38 TFAQAAGGEE426 pKa = 4.18 AEE428 pKa = 4.47 AVGFSLVGSTTISSLVLNRR447 pKa = 11.84 SYY449 pKa = 11.07 ILSSAVATDD458 pKa = 3.31 SDD460 pKa = 4.14 ASVGEE465 pKa = 4.37 AYY467 pKa = 10.27 AAGLIIGVDD476 pKa = 3.74 TVAEE480 pKa = 4.4 NISLVGSHH488 pKa = 6.4 LMSSAHH494 pKa = 5.77 AQTGEE499 pKa = 3.9 AVSYY503 pKa = 10.6 GVFVEE508 pKa = 5.03 DD509 pKa = 4.8 YY510 pKa = 10.63 YY511 pKa = 11.71 GGTPEE516 pKa = 3.95 VTLNLDD522 pKa = 3.13 AHH524 pKa = 6.54 SGILADD530 pKa = 3.61 WAVYY534 pKa = 8.33 TADD537 pKa = 4.55 GVVHH541 pKa = 6.73 LNNSGTLAGRR551 pKa = 11.84 LLVSDD556 pKa = 4.09 LTNNVGGRR564 pKa = 11.84 FMLLLDD570 pKa = 5.02 SDD572 pKa = 4.71 DD573 pKa = 4.55 LLIGEE578 pKa = 5.12 DD579 pKa = 3.56 GVGYY583 pKa = 10.73 FEE585 pKa = 6.64 DD586 pKa = 3.62 EE587 pKa = 4.03 DD588 pKa = 5.64 FYY590 pKa = 11.75 FSVADD595 pKa = 4.06 RR596 pKa = 11.84 AEE598 pKa = 4.25 LADD601 pKa = 3.42 GTTFLVYY608 pKa = 10.36 PSDD611 pKa = 3.48 NLGIVSEE618 pKa = 4.52 GDD620 pKa = 3.48 STSIALLSAGEE631 pKa = 4.15 GDD633 pKa = 3.19 WTLEE637 pKa = 3.96 NLSLEE642 pKa = 4.61 TPFDD646 pKa = 3.56 TPMIGLEE653 pKa = 4.13 LTEE656 pKa = 4.19 NAAGNQLIATATFLTPEE673 pKa = 4.03 QAGLSANATAAYY685 pKa = 7.53 EE686 pKa = 3.99 AAVDD690 pKa = 3.96 GGLFTPTSSPEE701 pKa = 3.58 EE702 pKa = 3.8 WSPNVSGAFVVGMAHH717 pKa = 6.25 TLMVSNMNIGNRR729 pKa = 11.84 LGMLSGMNSGDD740 pKa = 3.98 EE741 pKa = 4.04 IAASNGMWFSARR753 pKa = 11.84 FTDD756 pKa = 4.37 AEE758 pKa = 3.84 QDD760 pKa = 3.24 RR761 pKa = 11.84 RR762 pKa = 11.84 DD763 pKa = 4.04 GIEE766 pKa = 4.8 GFDD769 pKa = 4.28 ADD771 pKa = 4.0 TDD773 pKa = 4.0 ALSFGFDD780 pKa = 2.97 RR781 pKa = 11.84 EE782 pKa = 4.17 FGNVVLGVAYY792 pKa = 9.48 TRR794 pKa = 11.84 GNSDD798 pKa = 3.17 AKK800 pKa = 11.36 ANDD803 pKa = 3.29 ASADD807 pKa = 3.61 FEE809 pKa = 4.3 MSDD812 pKa = 4.51 NLFSLYY818 pKa = 10.72 GSYY821 pKa = 10.92 DD822 pKa = 2.91 GGTWYY827 pKa = 10.85 SEE829 pKa = 3.98 AVVSVGFGSVDD840 pKa = 3.25 SLRR843 pKa = 11.84 SVGSDD848 pKa = 4.03 DD849 pKa = 3.77 YY850 pKa = 12.04 AGDD853 pKa = 3.98 YY854 pKa = 11.19 DD855 pKa = 3.94 STSYY859 pKa = 9.57 STLVEE864 pKa = 4.38 SGCKK868 pKa = 9.38 ISAAGWEE875 pKa = 4.21 IYY877 pKa = 9.93 PYY879 pKa = 9.83 LTLEE883 pKa = 4.24 YY884 pKa = 10.39 SSKK887 pKa = 11.09 DD888 pKa = 3.21 YY889 pKa = 11.32 DD890 pKa = 3.86 SYY892 pKa = 11.7 TEE894 pKa = 4.17 TGGSLALHH902 pKa = 6.09 VRR904 pKa = 11.84 SKK906 pKa = 10.87 DD907 pKa = 3.63 YY908 pKa = 11.32 EE909 pKa = 4.26 VFTAGVGARR918 pKa = 11.84 LQKK921 pKa = 10.56 DD922 pKa = 4.09 FQRR925 pKa = 11.84 SWGIVTPEE933 pKa = 3.74 VSGQLAYY940 pKa = 10.39 DD941 pKa = 3.72 VEE943 pKa = 4.32 NDD945 pKa = 4.5 RR946 pKa = 11.84 IVSTANFVGGTTSFVAKK963 pKa = 10.18 GIEE966 pKa = 3.9 PSEE969 pKa = 4.24 TSWDD973 pKa = 3.24 LGAALTVASLGEE985 pKa = 4.12 QNVSLRR991 pKa = 11.84 LGYY994 pKa = 10.49 DD995 pKa = 3.12 YY996 pKa = 11.49 AGRR999 pKa = 11.84 QDD1001 pKa = 3.84 FAAHH1005 pKa = 6.4 SFTGKK1010 pKa = 9.76 VRR1012 pKa = 11.84 FEE1014 pKa = 4.22 FF1015 pKa = 4.5
Molecular weight: 106.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.605
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.948
Patrickios 1.1
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|Q1JZH4|Q1JZH4_DESA6 7-cyano-7-deazaguanine synthase OS=Desulfuromonas acetoxidans (strain DSM 684 / 11070) OX=281689 GN=queC PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.19 QPSRR9 pKa = 11.84 VSRR12 pKa = 11.84 KK13 pKa = 7.52 RR14 pKa = 11.84 THH16 pKa = 6.3 GFRR19 pKa = 11.84 KK20 pKa = 9.97 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 NVIKK32 pKa = 10.48 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 NRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.71 VLAATISSKK49 pKa = 11.21
Molecular weight: 5.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3204
0
3204
1103131
36
12388
344.3
38.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.798 ± 0.055
1.378 ± 0.024
5.949 ± 0.068
6.333 ± 0.048
3.907 ± 0.039
7.18 ± 0.073
2.391 ± 0.031
5.819 ± 0.035
4.242 ± 0.062
10.766 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.488 ± 0.029
3.448 ± 0.029
4.129 ± 0.049
4.693 ± 0.045
5.637 ± 0.067
6.049 ± 0.066
5.472 ± 0.07
7.352 ± 0.051
1.108 ± 0.02
2.859 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here