Woeseia oceani
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3565 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A193LBU3|A0A193LBU3_9GAMM Transcriptional regulator OS=Woeseia oceani OX=1548547 GN=BA177_01085 PE=4 SV=1
MM1 pKa = 7.05 RR2 pKa = 11.84 TKK4 pKa = 10.66 LALTAVVASIVAAGCGSGDD23 pKa = 3.52 INIDD27 pKa = 3.26 PVTSVTNSNNTTNTGGNSGTTNPCASYY54 pKa = 11.12 QNTGGQSIQGTYY66 pKa = 10.88 DD67 pKa = 3.36 GTHH70 pKa = 5.87 CTYY73 pKa = 10.97 SPSFVDD79 pKa = 3.44 AGNNLMVDD87 pKa = 4.26 LTLADD92 pKa = 4.27 LPNDD96 pKa = 3.93 GAHH99 pKa = 6.43 LFEE102 pKa = 5.68 GSLFVGEE109 pKa = 4.51 SHH111 pKa = 7.89 DD112 pKa = 4.44 NDD114 pKa = 3.99 ADD116 pKa = 3.85 LAAAGIAEE124 pKa = 4.7 GGDD127 pKa = 3.81 GPVLTVEE134 pKa = 4.77 AGATLALQSSASFMIINRR152 pKa = 11.84 GSQLFAVGRR161 pKa = 11.84 ADD163 pKa = 3.99 APITFTSLSDD173 pKa = 3.24 INGTVGPEE181 pKa = 4.69 DD182 pKa = 3.58 VQQWGGMVINGFGITNKK199 pKa = 9.87 CAYY202 pKa = 8.0 TGSVATNDD210 pKa = 3.88 LATSDD215 pKa = 3.93 CHH217 pKa = 8.07 VDD219 pKa = 3.23 AEE221 pKa = 4.75 GAAGLDD227 pKa = 3.53 EE228 pKa = 4.32 SQYY231 pKa = 11.76 GGDD234 pKa = 4.07 NNDD237 pKa = 3.79 DD238 pKa = 3.31 SSGRR242 pKa = 11.84 MEE244 pKa = 4.38 YY245 pKa = 10.83 VVVKK249 pKa = 8.84 HH250 pKa = 6.12 TGATVGNGDD259 pKa = 4.29 EE260 pKa = 4.65 LNGITFGGVGRR271 pKa = 11.84 NTIVNNLQVYY281 pKa = 7.69 STFDD285 pKa = 3.23 DD286 pKa = 5.04 GIEE289 pKa = 4.08 MFGGAVNFNNFVGMYY304 pKa = 9.88 VRR306 pKa = 11.84 DD307 pKa = 3.76 DD308 pKa = 5.08 AIDD311 pKa = 3.42 IDD313 pKa = 4.06 EE314 pKa = 5.49 GYY316 pKa = 10.82 SGTITNALVIQSEE329 pKa = 4.59 TDD331 pKa = 3.22 GNHH334 pKa = 6.8 CIEE337 pKa = 4.76 ADD339 pKa = 4.17 GIGGFDD345 pKa = 4.11 DD346 pKa = 4.97 LLPAEE351 pKa = 4.46 VEE353 pKa = 4.1 AMISQGINSRR363 pKa = 11.84 FVINNLTCIISPNGAATATHH383 pKa = 7.11 DD384 pKa = 4.31 PGAGWRR390 pKa = 11.84 LRR392 pKa = 11.84 EE393 pKa = 4.02 GLFAEE398 pKa = 4.93 INDD401 pKa = 3.93 SLLIGSMPANDD412 pKa = 3.04 QVSSNDD418 pKa = 3.55 NYY420 pKa = 10.93 CLRR423 pKa = 11.84 IDD425 pKa = 4.77 NRR427 pKa = 11.84 TQQAALDD434 pKa = 4.04 GDD436 pKa = 3.97 LTLNSVVFACAEE448 pKa = 3.92 ATRR451 pKa = 11.84 GQTFADD457 pKa = 3.93 GTTTEE462 pKa = 4.22 RR463 pKa = 11.84 SFAEE467 pKa = 3.74 AGGNIFATIANNTSVDD483 pKa = 3.69 PTAMNDD489 pKa = 3.07 AGLQLLEE496 pKa = 4.34 GTQPIYY502 pKa = 10.93 SIGYY506 pKa = 5.37 TTMQVDD512 pKa = 4.73 GAAPAGAPQSGTFIGGLSLSEE533 pKa = 4.38 TDD535 pKa = 2.97 WTMGWTFGLHH545 pKa = 6.46 PDD547 pKa = 3.83 NRR549 pKa = 11.84 SQALWFEE556 pKa = 4.42
Molecular weight: 57.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.567
ProMoST 3.948
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.923
Patrickios 0.935
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|A0A193LLG2|A0A193LLG2_9GAMM UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2 6-diaminopimelate ligase OS=Woeseia oceani OX=1548547 GN=murE PE=3 SV=1
MM1 pKa = 7.12 ATKK4 pKa = 10.22 KK5 pKa = 10.4 KK6 pKa = 10.33 SSKK9 pKa = 8.96 KK10 pKa = 7.15 TSKK13 pKa = 10.58 RR14 pKa = 11.84 KK15 pKa = 7.36 TATKK19 pKa = 10.38 RR20 pKa = 11.84 KK21 pKa = 6.26 VTKK24 pKa = 10.48 KK25 pKa = 10.01 KK26 pKa = 8.2 VAKK29 pKa = 10.22 RR30 pKa = 11.84 KK31 pKa = 8.09 VAKK34 pKa = 10.4 KK35 pKa = 9.96 KK36 pKa = 8.15 VAKK39 pKa = 10.35 RR40 pKa = 11.84 KK41 pKa = 5.75 TAKK44 pKa = 10.38 KK45 pKa = 9.83 KK46 pKa = 8.04 VAKK49 pKa = 10.36 RR50 pKa = 11.84 KK51 pKa = 7.12 TKK53 pKa = 10.3 KK54 pKa = 9.6 KK55 pKa = 10.07 AKK57 pKa = 10.03 KK58 pKa = 9.6 KK59 pKa = 7.94 VAKK62 pKa = 10.16 RR63 pKa = 11.84 KK64 pKa = 7.15 TKK66 pKa = 10.59 KK67 pKa = 10.07 KK68 pKa = 7.92 VAKK71 pKa = 10.28 RR72 pKa = 11.84 KK73 pKa = 7.12 TKK75 pKa = 10.45 KK76 pKa = 9.7 KK77 pKa = 10.16 AKK79 pKa = 9.78 KK80 pKa = 8.96 KK81 pKa = 9.84 AKK83 pKa = 10.16 KK84 pKa = 9.76 KK85 pKa = 7.97 VAKK88 pKa = 10.25 RR89 pKa = 11.84 KK90 pKa = 7.28 TKK92 pKa = 10.6 KK93 pKa = 8.87 KK94 pKa = 8.18 TKK96 pKa = 10.19 KK97 pKa = 9.75 KK98 pKa = 9.46 AAKK101 pKa = 9.99 KK102 pKa = 8.92 KK103 pKa = 6.97 TAKK106 pKa = 10.28 KK107 pKa = 9.56 KK108 pKa = 10.27 AKK110 pKa = 9.88 KK111 pKa = 9.32 KK112 pKa = 9.89 AKK114 pKa = 10.21 KK115 pKa = 9.86 KK116 pKa = 8.69 VAKK119 pKa = 9.99 KK120 pKa = 8.82 KK121 pKa = 10.54 AKK123 pKa = 10.25 RR124 pKa = 11.84 KK125 pKa = 8.65 VKK127 pKa = 10.49 KK128 pKa = 10.01 KK129 pKa = 10.11 ASKK132 pKa = 10.4 KK133 pKa = 8.89 KK134 pKa = 9.15 ATRR137 pKa = 11.84 KK138 pKa = 6.94 TRR140 pKa = 11.84 KK141 pKa = 9.32 KK142 pKa = 10.45 ASKK145 pKa = 10.29 KK146 pKa = 9.93 KK147 pKa = 10.32
Molecular weight: 16.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.428
IPC2_protein 11.506
IPC_protein 12.852
Toseland 13.1
ProMoST 13.51
Dawson 13.115
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 13.466
Grimsley 13.144
Solomon 13.539
Lehninger 13.451
Nozaki 13.086
DTASelect 13.042
Thurlkill 13.086
EMBOSS 13.568
Sillero 13.086
Patrickios 13.173
IPC_peptide 13.539
IPC2_peptide 12.501
IPC2.peptide.svr19 9.136
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3565
0
3565
1201765
51
1901
337.1
36.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.096 ± 0.045
0.916 ± 0.016
6.191 ± 0.032
5.933 ± 0.034
3.704 ± 0.026
7.942 ± 0.034
2.032 ± 0.019
5.198 ± 0.032
3.024 ± 0.033
10.319 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.342 ± 0.019
3.332 ± 0.024
4.563 ± 0.024
3.675 ± 0.025
6.87 ± 0.03
6.169 ± 0.03
5.252 ± 0.031
7.387 ± 0.032
1.331 ± 0.017
2.725 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here