Lihuaxuella thermophila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Lihuaxuella

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3710 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8JQ81|A0A1H8JQ81_9BACL Cytosine-specific methyltransferase OS=Lihuaxuella thermophila OX=1173111 GN=SAMN05444955_1361 PE=3 SV=1
MM1 pKa = 7.53VPDD4 pKa = 3.56EE5 pKa = 4.22VVMVFRR11 pKa = 11.84MPCPVGEE18 pKa = 4.56GGDD21 pKa = 3.8GRR23 pKa = 11.84STAVLDD29 pKa = 4.05RR30 pKa = 11.84GEE32 pKa = 4.29TVLGIIHH39 pKa = 6.87VGTISITDD47 pKa = 3.61QIPVGVIGIACPVDD61 pKa = 3.64CGQLISVIVGIGGCGCCSPGRR82 pKa = 11.84SYY84 pKa = 11.48FLGEE88 pKa = 4.17TVSDD92 pKa = 3.89GVIGVFIDD100 pKa = 3.77GAIRR104 pKa = 11.84SGFFDD109 pKa = 3.56EE110 pKa = 5.32LVEE113 pKa = 4.2FVVDD117 pKa = 3.57IGGGSSIGYY126 pKa = 7.33DD127 pKa = 2.84TGDD130 pKa = 2.96IFFGIVGVLAVDD142 pKa = 3.78HH143 pKa = 5.92LTIFF147 pKa = 3.93

Molecular weight: 15.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8AV13|A0A1H8AV13_9BACL Bacterioferritin OS=Lihuaxuella thermophila OX=1173111 GN=SAMN05444955_101333 PE=3 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84RR3 pKa = 11.84QTVFTMILGGLLALIASRR21 pKa = 11.84WVRR24 pKa = 11.84PRR26 pKa = 11.84NRR28 pKa = 11.84MDD30 pKa = 5.3LIRR33 pKa = 11.84RR34 pKa = 11.84AVSMGRR40 pKa = 11.84EE41 pKa = 3.45IRR43 pKa = 11.84QNVFFGRR50 pKa = 11.84LVSGAVARR58 pKa = 11.84KK59 pKa = 9.23LLRR62 pKa = 11.84RR63 pKa = 11.84FQWARR68 pKa = 3.09

Molecular weight: 8.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3710

0

3710

1064601

25

2748

287.0

32.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.757 ± 0.045

0.878 ± 0.014

4.81 ± 0.028

7.23 ± 0.044

4.123 ± 0.033

7.363 ± 0.037

2.322 ± 0.021

6.705 ± 0.032

5.902 ± 0.04

10.127 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.684 ± 0.018

3.367 ± 0.028

4.492 ± 0.026

3.99 ± 0.027

5.73 ± 0.035

5.559 ± 0.03

5.026 ± 0.027

7.313 ± 0.03

1.33 ± 0.021

3.292 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski